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Protein

2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene

menH

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC).UniRule annotation

Catalytic activityi

5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate.UniRule annotation

Pathwayi: 1,4-dihydroxy-2-naphthoate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD)
  3. 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH)
  4. o-succinylbenzoate synthase (menC)
  5. no protein annotated in this organism
  6. 1,4-dihydroxy-2-naphthoyl-CoA synthase (menB)
  7. 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (ydiI)
This subpathway is part of the pathway 1,4-dihydroxy-2-naphthoate biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate, the pathway 1,4-dihydroxy-2-naphthoate biosynthesis and in Quinol/quinone metabolism.

Pathwayi: menaquinone biosynthesis

This protein is involved in the pathway menaquinone biosynthesis, which is part of Quinol/quinone metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Menaquinone biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00079.
UPA01057; UER00900.

Protein family/group databases

ESTHERiklep7-menh. MenH_SHCHC.
MEROPSiS33.996.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthaseUniRule annotation (EC:4.2.99.20UniRule annotation)
Short name:
SHCHC synthaseUniRule annotation
Gene namesi
Name:menHUniRule annotation
Ordered Locus Names:KPN78578_26170
ORF Names:KPN_02661
OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Taxonomic identifieri272620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000000265 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003419151 – 2522-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthaseAdd BLAST252

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272620.KPN_02661.

Structurei

3D structure databases

ProteinModelPortaliA6TBV7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. MenH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107J5P. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028072.
KOiK08680.
OMAiLNDWYQQ.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01660. MenH. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR022485. SHCHC_synthase_MenH.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR03695. menH_SHCHC. 1 hit.

Sequencei

Sequence statusi: Complete.

A6TBV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILSAAVDNG QPGYPWLVFL HGFSGDRNEW RKVGDAFPAW PRLYLDLPGH
60 70 80 90 100
GGSADIAVQD FAGVNTLLQS TLNSYNIHKY WLIGYSLGGR VAMNFASQPR
110 120 130 140 150
AGMRGLIVEG GHPGLQDAEA RQARRSNDSA WAERFRREPL EQVFADWYQQ
160 170 180 190 200
PVFASLNAAQ RESLVALRSR NNGATLAAML QATSLAAQAD LRASLQARDF
210 220 230 240 250
PFHYLCGERD AKFRAIAQTL AADLHLIHHA GHNAHRDNPA AVIACLAQIL

AS
Length:252
Mass (Da):27,655
Last modified:August 21, 2007 - v1
Checksum:i4F58C4E3A3D85C55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR78078.1.
RefSeqiWP_015958756.1. NC_009648.1.

Genome annotation databases

EnsemblBacteriaiABR78078; ABR78078; KPN_02661.
KEGGikpn:KPN_02661.
PATRICi20459618. VBIKlePne13394_2683.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR78078.1.
RefSeqiWP_015958756.1. NC_009648.1.

3D structure databases

ProteinModelPortaliA6TBV7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272620.KPN_02661.

Protein family/group databases

ESTHERiklep7-menh. MenH_SHCHC.
MEROPSiS33.996.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR78078; ABR78078; KPN_02661.
KEGGikpn:KPN_02661.
PATRICi20459618. VBIKlePne13394_2683.

Phylogenomic databases

eggNOGiENOG4107J5P. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028072.
KOiK08680.
OMAiLNDWYQQ.

Enzyme and pathway databases

UniPathwayiUPA00079.
UPA01057; UER00900.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01660. MenH. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR022485. SHCHC_synthase_MenH.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR03695. menH_SHCHC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMENH_KLEP7
AccessioniPrimary (citable) accession number: A6TBV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: August 21, 2007
Last modified: November 2, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.