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Protein

L-rhamnonate dehydratase

Gene

rhmD

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the dehydration of L-rhamnonate to 2-keto-3-deoxy-L-rhamnonate (KDR).UniRule annotation

Catalytic activityi

L-rhamnonate = 2-dehydro-3-deoxy-L-rhamnonate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei29SubstrateUniRule annotation1
Binding sitei55SubstrateUniRule annotation1
Metal bindingi222MagnesiumUniRule annotation1
Metal bindingi248MagnesiumUniRule annotation1
Metal bindingi276MagnesiumUniRule annotation1
Sitei298Increases basicity of active site HisUniRule annotation1
Active sitei325Proton acceptorUniRule annotation1
Binding sitei345SubstrateUniRule annotation1
Sitei345Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
L-rhamnonate dehydrataseUniRule annotation (EC:4.2.1.90UniRule annotation)
Short name:
RhamDUniRule annotation
Gene namesi
Name:rhmDUniRule annotation
Ordered Locus Names:KPN78578_26080
ORF Names:KPN_02652
OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Taxonomic identifieri272620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000000265 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003517001 – 401L-rhamnonate dehydrataseAdd BLAST401

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi272620.KPN_02652.

Structurei

3D structure databases

ProteinModelPortaliA6TBU8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CXK. Bacteria.
COG4948. LUCA.
HOGENOMiHOG000113755.
KOiK12661.
OMAiINWWEEC.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_01288. Rhamnon_dehydrat. 1 hit.
InterProiIPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR023444. L-Rhamnon_dehydrat.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PfamiPF13378. MR_MLE_C. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
PROSITEiPS00908. MR_MLE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6TBU8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLPKIKHVR AWFIGGATAE QGAGGGDYHD QGANHWIDDH IATPMSKYKQ
60 70 80 90 100
YEQSRQSFGI NVLGTLIVEV EADNGQTGFA VSTAGEMGCF IVEKHLNRFI
110 120 130 140 150
EGKCVSDIKL IHDQMLNATL YYAGSGGLVM NTISCVDLAL WDLFGKVVGL
160 170 180 190 200
PVYKLLGGAV RDEIQFYATG ARPDLAQEMG FIGGKMPTHW GPHDGDAGIR
210 220 230 240 250
KDVAMVADMR EKCGPDFWLM LDCWMSQDVN YATKLAHACA PYNLKWIEEC
260 270 280 290 300
LPPQQYEGYR ELKRQAPAGM MVTSGEHHGT LQSFRTLSET GIDIMQPDVG
310 320 330 340 350
WCGGLTTLVE IAAIAKARGQ LVVPHGSSVY SHHAVITFTN TPFSEFLMTS
360 370 380 390 400
PDCATLRPQF DPILLGEPVP ERGRIHKSVL DKPGFGVELN RDCNLKRPYQ

H
Length:401
Mass (Da):44,202
Last modified:August 21, 2007 - v1
Checksum:iAFB4385C9AF18AB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR78069.1.
RefSeqiWP_004175029.1. NC_009648.1.

Genome annotation databases

EnsemblBacteriaiABR78069; ABR78069; KPN_02652.
KEGGikpn:KPN_02652.
PATRICi20459598. VBIKlePne13394_2673.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR78069.1.
RefSeqiWP_004175029.1. NC_009648.1.

3D structure databases

ProteinModelPortaliA6TBU8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272620.KPN_02652.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR78069; ABR78069; KPN_02652.
KEGGikpn:KPN_02652.
PATRICi20459598. VBIKlePne13394_2673.

Phylogenomic databases

eggNOGiENOG4105CXK. Bacteria.
COG4948. LUCA.
HOGENOMiHOG000113755.
KOiK12661.
OMAiINWWEEC.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_01288. Rhamnon_dehydrat. 1 hit.
InterProiIPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR023444. L-Rhamnon_dehydrat.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PfamiPF13378. MR_MLE_C. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
PROSITEiPS00908. MR_MLE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHMD_KLEP7
AccessioniPrimary (citable) accession number: A6TBU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: August 21, 2007
Last modified: November 2, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Reaction proceeds via a syn dehydration.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.