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Protein

L-rhamnonate dehydratase

Gene

rhmD

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the dehydration of L-rhamnonate to 2-keto-3-deoxy-L-rhamnonate (KDR).UniRule annotation

Miscellaneous

Reaction proceeds via a syn dehydration.UniRule annotation

Catalytic activityi

L-rhamnonate = 2-dehydro-3-deoxy-L-rhamnonate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei29SubstrateUniRule annotation1
Binding sitei55SubstrateUniRule annotation1
Metal bindingi222MagnesiumUniRule annotation1
Metal bindingi248MagnesiumUniRule annotation1
Metal bindingi276MagnesiumUniRule annotation1
Sitei298Increases basicity of active site HisUniRule annotation1
Active sitei325Proton acceptorUniRule annotation1
Binding sitei345SubstrateUniRule annotation1
Sitei345Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
L-rhamnonate dehydrataseUniRule annotation (EC:4.2.1.90UniRule annotation)
Short name:
RhamDUniRule annotation
Gene namesi
Name:rhmDUniRule annotation
Ordered Locus Names:KPN78578_26080
ORF Names:KPN_02652
OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Taxonomic identifieri272620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000000265 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003517001 – 401L-rhamnonate dehydrataseAdd BLAST401

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi272620.KPN_02652

Structurei

3D structure databases

ProteinModelPortaliA6TBU8
SMRiA6TBU8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CXK Bacteria
COG4948 LUCA
HOGENOMiHOG000113755
KOiK12661
OMAiNINWWEE

Family and domain databases

Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_01288 Rhamnon_dehydrat, 1 hit
InterProiView protein in InterPro
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR029065 Enolase_C-like
IPR023444 L-Rhamnon_dehydrat
IPR018110 Mandel_Rmase/mucon_lact_enz_CS
IPR034593 Mandelate_racemase-like
IPR013342 Mandelate_racemase_C
IPR013341 Mandelate_racemase_N_dom
PANTHERiPTHR13794:SF59 PTHR13794:SF59, 1 hit
PfamiView protein in Pfam
PF13378 MR_MLE_C, 1 hit
PF02746 MR_MLE_N, 1 hit
SFLDiSFLDF00006 rhamnonate_dehydratase, 1 hit
SFLDG00179 mandelate_racemase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM00922 MR_MLE, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
PROSITEiView protein in PROSITE
PS00908 MR_MLE_1, 1 hit

Sequencei

Sequence statusi: Complete.

A6TBU8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLPKIKHVR AWFIGGATAE QGAGGGDYHD QGANHWIDDH IATPMSKYKQ
60 70 80 90 100
YEQSRQSFGI NVLGTLIVEV EADNGQTGFA VSTAGEMGCF IVEKHLNRFI
110 120 130 140 150
EGKCVSDIKL IHDQMLNATL YYAGSGGLVM NTISCVDLAL WDLFGKVVGL
160 170 180 190 200
PVYKLLGGAV RDEIQFYATG ARPDLAQEMG FIGGKMPTHW GPHDGDAGIR
210 220 230 240 250
KDVAMVADMR EKCGPDFWLM LDCWMSQDVN YATKLAHACA PYNLKWIEEC
260 270 280 290 300
LPPQQYEGYR ELKRQAPAGM MVTSGEHHGT LQSFRTLSET GIDIMQPDVG
310 320 330 340 350
WCGGLTTLVE IAAIAKARGQ LVVPHGSSVY SHHAVITFTN TPFSEFLMTS
360 370 380 390 400
PDCATLRPQF DPILLGEPVP ERGRIHKSVL DKPGFGVELN RDCNLKRPYQ

H
Length:401
Mass (Da):44,202
Last modified:August 21, 2007 - v1
Checksum:iAFB4385C9AF18AB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA Translation: ABR78069.1
RefSeqiWP_004175029.1, NC_009648.1

Genome annotation databases

EnsemblBacteriaiABR78069; ABR78069; KPN_02652
KEGGikpn:KPN_02652

Similar proteinsi

Entry informationi

Entry nameiRHMD_KLEP7
AccessioniPrimary (citable) accession number: A6TBU8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: August 21, 2007
Last modified: March 28, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health