Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A6TB47 (OTSA_KLEP7) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

EC=2.4.1.15
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene names
Name:otsA
Ordered Locus Names:KPN78578_23570
ORF Names:KPN_02392
OrganismKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]
Taxonomic identifier272620 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor By similarity.

Catalytic activity

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathway

Glycan biosynthesis; trehalose biosynthesis.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtrehalose biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionalpha,alpha-trehalose-phosphate synthase (UDP-forming) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 474474Alpha,alpha-trehalose-phosphate synthase [UDP-forming]
PRO_0000348902

Regions

Region22 – 232UDP-glucose binding By similarity
Region366 – 3705UDP-glucose binding By similarity

Sites

Binding site101Glucose-6-phosphate By similarity
Binding site771Glucose-6-phosphate By similarity
Binding site1311Glucose-6-phosphate By similarity
Binding site2631UDP-glucose By similarity
Binding site2681UDP-glucose By similarity
Binding site3011Glucose-6-phosphate By similarity
Binding site3401UDP-glucose; via amide nitrogen and carbonyl oxygen By similarity
Site861Involved in alpha anomer selectivity By similarity
Site1561Involved in alpha anomer selectivity By similarity

Sequences

Sequence LengthMass (Da)Tools
A6TB47 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: B022E7320D3BBD79

FASTA47453,813
        10         20         30         40         50         60 
MSRLVVVSNR IALPDDKKSS AGGLAVGILG ALRAAGGLWF GWSGEIGDDQ QPLKQVSRGN 

        70         80         90        100        110        120 
ISWASFNLNE RDHDEYYNQF SNAVLWPAFH YRLDLVSFQR EAWEGYLRVN AMLADKLLPL 

       130        140        150        160        170        180 
IEPDDTLWIH DYHLLPFASE LRKRGVNNRI GFFLHIPFPT PEIFNALPPH AELLEQLCDY 

       190        200        210        220        230        240 
DLLGFQTESD RTAFLDSIAM QTRLSDLGDK RYQAWGKAFS TEVYPIGIDP DEITRNAKGP 

       250        260        270        280        290        300 
LPPKLAQLKN ELKNVKNIFS VERLDYSKGL PERFLAYETL LEKYPQHHGK IRYTQIAPTS 

       310        320        330        340        350        360 
RGDVQAYQDI RHQLETAAGR INGQFGQLGW TPLYYLNQHF DRKLLMKVFR YSDVGLVTPL 

       370        380        390        400        410        420 
RDGMNLVAKE YVAAQDPDNP GVLVLSQFAG AAQELTSALI VNPYDRDEVA AALDRALSMP 

       430        440        450        460        470 
LAERIARHSA MLDVIRENDI HNWQARFVED LQHISPRSEE SRLRGKIATF PKLA 

« Hide

References

[1]The Klebsiella pneumonia Genome Sequencing Project
McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.
Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700721 / MGH 78578.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000647 Genomic DNA. Translation: ABR77818.1.
RefSeqYP_001336048.1. NC_009648.1.

3D structure databases

ProteinModelPortalA6TB47.
SMRA6TB47. Positions 2-457.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272620.KPN_02392.

Protein family/group databases

CAZyGT20. Glycosyltransferase Family 20.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR77818; ABR77818; KPN_02392.
GeneID5338205.
KEGGkpn:KPN_02392.
PATRIC20459078. VBIKlePne13394_2422.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0380.
HOGENOMHOG000191478.
KOK00697.
OMAWGESFVK.
OrthoDBEOG6CCH1M.

Enzyme and pathway databases

BioCycKPNE272620:GKDC-2392-MONOMER.
UniPathwayUPA00299.

Family and domain databases

InterProIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsTIGR02400. trehalose_OtsA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameOTSA_KLEP7
AccessionPrimary (citable) accession number: A6TB47
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: August 21, 2007
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways