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A6TB47

- OTSA_KLEP7

UniProt

A6TB47 - OTSA_KLEP7

Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

Gene

otsA

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor By similarity.By similarity

    Catalytic activityi

    UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101Glucose-6-phosphateBy similarity
    Binding sitei77 – 771Glucose-6-phosphateBy similarity
    Sitei86 – 861Involved in alpha anomer selectivityBy similarity
    Binding sitei131 – 1311Glucose-6-phosphateBy similarity
    Sitei156 – 1561Involved in alpha anomer selectivityBy similarity
    Binding sitei263 – 2631UDP-glucoseBy similarity
    Binding sitei268 – 2681UDP-glucoseBy similarity
    Binding sitei301 – 3011Glucose-6-phosphateBy similarity
    Binding sitei340 – 3401UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: UniProtKB-EC

    GO - Biological processi

    1. trehalose biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciKPNE272620:GKDC-2392-MONOMER.
    UniPathwayiUPA00299.

    Protein family/group databases

    CAZyiGT20. Glycosyltransferase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
    Alternative name(s):
    Osmoregulatory trehalose synthesis protein A
    Trehalose-6-phosphate synthase
    UDP-glucose-glucosephosphate glucosyltransferase
    Gene namesi
    Name:otsA
    Ordered Locus Names:KPN78578_23570
    ORF Names:KPN_02392
    OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
    Taxonomic identifieri272620 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
    ProteomesiUP000000265: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 474474Alpha,alpha-trehalose-phosphate synthase [UDP-forming]PRO_0000348902Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi272620.KPN_02392.

    Structurei

    3D structure databases

    ProteinModelPortaliA6TB47.
    SMRiA6TB47. Positions 2-457.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni22 – 232UDP-glucose bindingBy similarity
    Regioni366 – 3705UDP-glucose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 20 family.Curated

    Phylogenomic databases

    eggNOGiCOG0380.
    HOGENOMiHOG000191478.
    KOiK00697.
    OMAiWGESFVK.
    OrthoDBiEOG6CCH1M.

    Family and domain databases

    InterProiIPR001830. Glyco_trans_20.
    IPR012766. Trehalose_OtsA.
    [Graphical view]
    PfamiPF00982. Glyco_transf_20. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A6TB47-1 [UniParc]FASTAAdd to Basket

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    MSRLVVVSNR IALPDDKKSS AGGLAVGILG ALRAAGGLWF GWSGEIGDDQ    50
    QPLKQVSRGN ISWASFNLNE RDHDEYYNQF SNAVLWPAFH YRLDLVSFQR 100
    EAWEGYLRVN AMLADKLLPL IEPDDTLWIH DYHLLPFASE LRKRGVNNRI 150
    GFFLHIPFPT PEIFNALPPH AELLEQLCDY DLLGFQTESD RTAFLDSIAM 200
    QTRLSDLGDK RYQAWGKAFS TEVYPIGIDP DEITRNAKGP LPPKLAQLKN 250
    ELKNVKNIFS VERLDYSKGL PERFLAYETL LEKYPQHHGK IRYTQIAPTS 300
    RGDVQAYQDI RHQLETAAGR INGQFGQLGW TPLYYLNQHF DRKLLMKVFR 350
    YSDVGLVTPL RDGMNLVAKE YVAAQDPDNP GVLVLSQFAG AAQELTSALI 400
    VNPYDRDEVA AALDRALSMP LAERIARHSA MLDVIRENDI HNWQARFVED 450
    LQHISPRSEE SRLRGKIATF PKLA 474
    Length:474
    Mass (Da):53,813
    Last modified:August 21, 2007 - v1
    Checksum:iB022E7320D3BBD79
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000647 Genomic DNA. Translation: ABR77818.1.
    RefSeqiYP_001336048.1. NC_009648.1.

    Genome annotation databases

    EnsemblBacteriaiABR77818; ABR77818; KPN_02392.
    GeneIDi5338205.
    KEGGikpn:KPN_02392.
    PATRICi20459078. VBIKlePne13394_2422.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000647 Genomic DNA. Translation: ABR77818.1 .
    RefSeqi YP_001336048.1. NC_009648.1.

    3D structure databases

    ProteinModelPortali A6TB47.
    SMRi A6TB47. Positions 2-457.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272620.KPN_02392.

    Protein family/group databases

    CAZyi GT20. Glycosyltransferase Family 20.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR77818 ; ABR77818 ; KPN_02392 .
    GeneIDi 5338205.
    KEGGi kpn:KPN_02392.
    PATRICi 20459078. VBIKlePne13394_2422.

    Phylogenomic databases

    eggNOGi COG0380.
    HOGENOMi HOG000191478.
    KOi K00697.
    OMAi WGESFVK.
    OrthoDBi EOG6CCH1M.

    Enzyme and pathway databases

    UniPathwayi UPA00299 .
    BioCyci KPNE272620:GKDC-2392-MONOMER.

    Family and domain databases

    InterProi IPR001830. Glyco_trans_20.
    IPR012766. Trehalose_OtsA.
    [Graphical view ]
    Pfami PF00982. Glyco_transf_20. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02400. trehalose_OtsA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700721 / MGH 78578.

    Entry informationi

    Entry nameiOTSA_KLEP7
    AccessioniPrimary (citable) accession number: A6TB47
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3