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Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

Gene

otsA

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor (By similarity).By similarity

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathwayi: trehalose biosynthesis

This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10Glucose-6-phosphateBy similarity1
Binding sitei77Glucose-6-phosphateBy similarity1
Sitei86Involved in alpha anomer selectivityBy similarity1
Binding sitei131Glucose-6-phosphateBy similarity1
Sitei156Involved in alpha anomer selectivityBy similarity1
Binding sitei263UDP-glucoseBy similarity1
Binding sitei268UDP-glucoseBy similarity1
Binding sitei301Glucose-6-phosphateBy similarity1
Binding sitei340UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene namesi
Name:otsA
Ordered Locus Names:KPN78578_23570
ORF Names:KPN_02392
OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Taxonomic identifieri272620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000000265 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003489021 – 474Alpha,alpha-trehalose-phosphate synthase [UDP-forming]Add BLAST474

Proteomic databases

PRIDEiA6TB47.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272620.KPN_02392.

Structurei

3D structure databases

ProteinModelPortaliA6TB47.
SMRiA6TB47.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 23UDP-glucose bindingBy similarity2
Regioni366 – 370UDP-glucose bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.Curated

Phylogenomic databases

eggNOGiENOG4105C1K. Bacteria.
COG0380. LUCA.
HOGENOMiHOG000191478.
KOiK00697.
OMAiIEFMPIH.

Family and domain databases

CDDicd03788. GT1_TPS. 1 hit.
InterProiIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

Sequencei

Sequence statusi: Complete.

A6TB47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLVVVSNR IALPDDKKSS AGGLAVGILG ALRAAGGLWF GWSGEIGDDQ
60 70 80 90 100
QPLKQVSRGN ISWASFNLNE RDHDEYYNQF SNAVLWPAFH YRLDLVSFQR
110 120 130 140 150
EAWEGYLRVN AMLADKLLPL IEPDDTLWIH DYHLLPFASE LRKRGVNNRI
160 170 180 190 200
GFFLHIPFPT PEIFNALPPH AELLEQLCDY DLLGFQTESD RTAFLDSIAM
210 220 230 240 250
QTRLSDLGDK RYQAWGKAFS TEVYPIGIDP DEITRNAKGP LPPKLAQLKN
260 270 280 290 300
ELKNVKNIFS VERLDYSKGL PERFLAYETL LEKYPQHHGK IRYTQIAPTS
310 320 330 340 350
RGDVQAYQDI RHQLETAAGR INGQFGQLGW TPLYYLNQHF DRKLLMKVFR
360 370 380 390 400
YSDVGLVTPL RDGMNLVAKE YVAAQDPDNP GVLVLSQFAG AAQELTSALI
410 420 430 440 450
VNPYDRDEVA AALDRALSMP LAERIARHSA MLDVIRENDI HNWQARFVED
460 470
LQHISPRSEE SRLRGKIATF PKLA
Length:474
Mass (Da):53,813
Last modified:August 21, 2007 - v1
Checksum:iB022E7320D3BBD79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR77818.1.
RefSeqiWP_004175414.1. NC_009648.1.

Genome annotation databases

EnsemblBacteriaiABR77818; ABR77818; KPN_02392.
KEGGikpn:KPN_02392.
PATRICi20459078. VBIKlePne13394_2422.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR77818.1.
RefSeqiWP_004175414.1. NC_009648.1.

3D structure databases

ProteinModelPortaliA6TB47.
SMRiA6TB47.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272620.KPN_02392.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Proteomic databases

PRIDEiA6TB47.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR77818; ABR77818; KPN_02392.
KEGGikpn:KPN_02392.
PATRICi20459078. VBIKlePne13394_2422.

Phylogenomic databases

eggNOGiENOG4105C1K. Bacteria.
COG0380. LUCA.
HOGENOMiHOG000191478.
KOiK00697.
OMAiIEFMPIH.

Enzyme and pathway databases

UniPathwayiUPA00299.

Family and domain databases

CDDicd03788. GT1_TPS. 1 hit.
InterProiIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiOTSA_KLEP7
AccessioniPrimary (citable) accession number: A6TB47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: August 21, 2007
Last modified: November 2, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.