A6TB43 (SYR_KLEP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	KPN78578_23530
ORF Names:	KPN_02388

Organism

Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]

Taxonomic identifier

272620 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella › Klebsiella pneumoniae › Klebsiella pneumoniae subsp. pneumoniae

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 577

577

Arginine--tRNA ligase HAMAP-Rule MF_00123

PRO_1000018046

Regions

Motif

122 – 132

11

"HIGH" region HAMAP-Rule MF_00123

Secondary structure

1

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577

Helix Strand Turn

Details...

Helix

3 – 17

15

Beta strand

28 – 30

3

Helix

34 – 36

3

Beta strand

38 – 41

4

Helix

44 – 50

7

Helix

55 – 65

11

Turn

69 – 71

3

Beta strand

72 – 78

7

Turn

79 – 81

3

Beta strand

82 – 87

6

Helix

89 – 100

12

Sequences

	Sequence		Length	Mass (Da)
	A6TB43 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 93D42215E0585926	FASTA	577	64,242
	10 20 30 40 50 60 MNIQALLSEK VSQALIAAGA PADCEPQVRQ SAKVQFGDYQ ANGVMAVAKK LGMAPRQLAE 70 80 90 100 110 120 QVLSHLDLNG IANKVEIAGP GFINIFLDPA FLADNVNRAL QSERLGVTKP QAQTIVVDYS 130 140 150 160 170 180 APNVAKEMHV GHLRSTIIGD ASVRTLEFLG HKVIRANHVG DWGTQFGMLI AYLEKQQQEN 190 200 210 220 230 240 AGEMALADLE GFYREAKKHY DEDEAFAERA RSYVVKLQGG DEYFLQMWRK LVDITMSQNQ 250 260 270 280 290 300 ITYDRLNVTL TRDDVMGESL YNPMLPGIVA DLKAKGLAVE SEGATVVFLD EYKNKEGEPM 310 320 330 340 350 360 GVIIQKKDGG YLYTTTDIAC AKYRYETLHA DRVLYYIDSR QHQHLMQAWT IVRKAGYVPD 370 380 390 400 410 420 SVPLEHHMFG MMLGKDGKPF KTRAGGTVKL ADLLDEALER ARRLVAEKNP DMSADELENL 430 440 450 460 470 480 AKVVGIGAVK YADLSKNRTT DYVFDWDNML AFEGNTAPYM QYAYTRVLSV FRKAGIDENA 490 500 510 520 530 540 MIDAPVVIAE DREAQLAARL LQFEETLSVV AREGTPHVMC AYLYDLAGLF SGFYEHCPIL 550 560 570 SAESEETRNS RLKLALLTAK TLKLGLDTLG IETVERM

References

[1]

The Klebsiella pneumonia Genome Sequencing Project McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700721 / MGH 78578.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CP000647 Genomic DNA. Translation: ABR77814.1.

RefSeq

YP_001336044.1. NC_009648.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3GDZ	X-ray	2.20	A/B/C/D	1-106	[»]

ProteinModelPortal

A6TB43.

ModBase

Search...

Protein-protein interaction databases

STRING

272620.KPN_02388.

Proteomic databases

PRIDE

A6TB43.

Protocols and materials databases

DNASU

5342538.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

ABR77814; ABR77814; KPN_02388.

GeneID

5342538.

KEGG

kpn:KPN_02388.

PATRIC

20459070. VBIKlePne13394_2418.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0018.

HOGENOM

HOG000247212.

KO

K01887.

OMA

QGAKCVF.

ProtClustDB

PRK01611.

Family and domain databases

Gene3D

3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.

HAMAP

MF_00123. Arg_tRNA_synth.

InterPro

IPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase_Ia.
IPR015945. Arg-tRNA-synth_Ia_core.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]

PANTHER

PTHR11956. PTHR11956. 1 hit.

Pfam

PF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]

PRINTS

PR01038. TRNASYNTHARG.

SMART

SM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]

SUPFAM

SSF55190. Arg-tRNA-synth_Ic_N. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.

TIGRFAMs

TIGR00456. argS. 1 hit.

PROSITE

PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

A6TB43.

Entry information

Entry name

SYR_KLEP7

Accession

Primary (citable) accession number: A6TB43

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program