Reviewed, UniProtKB/Swiss-Prot A6TAW4 (DADA_KLEP7)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	KPN78578_22740
ORF Names:	KPN_02309

Organism

Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]

Taxonomic identifier

272620 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity.
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000066097
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	A6TAW4-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 2C1E617E9B52F788	FASTA	432	47,231
	10 20 30 40 50 60 MRVVILGSGV VGVASAWYLS QAGHDVTVID RQPGPAEETS AANAGQISPG YAAPWAAPGV 70 80 90 100 110 120 PLKAIKWMFQ RHAPLAIGLD GTSFQLKWMW QMLRNCDTRH YMENKGRMVR LAEYSRDCLK 130 140 150 160 170 180 ALRDTTGIQY EGRQGGTLQL FRTAKQYENA TRDIAVLEDA GVPYQLLEAK RLAEVEPALA 190 200 210 220 230 240 EVSHKLTGGL RLPNDETGDC QLFTTRLAAM AEQAGVTFRF NTAVDALLHE GDRIAGVKCG 250 260 270 280 290 300 DEIIKGDAYV MAFGSYSTAM LKGLVDIPVY PLKGYSLTIP IAQEDGAPVS TILDETYKIA 310 320 330 340 350 360 ITRFDQRIRV GGMAEIVGFN KTLLQPRRET LEMVVRDLFP RGGHVEQATF WTGLRPMTPD 370 380 390 400 410 420 GTPVVGRTAY KNLWLNTGHG TLGWTMACGS GQLISDLISG RTPAIPYDDL AVARYSPGFT 430 PARPQHLHGA HN

References

[1]	McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000647 Genomic DNA. Translation: ABR77735.1.
RefSeq	YP_001335965.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6TAW4.
Genome annotation databases
GeneID	5338426.
GenomeReviews	Gene locus KPN78578_22740 in contig CP000647_GR.
KEGG	kpn:KPN_02309.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MFQKHAP.
Family and domain databases
HAMAP	MF_01202. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 3 hits.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

DADA_KLEP7

Accession

Primary (citable) accession number: A6TAW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)