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A6TAC8 (MHPB_KLEP7) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
EC=1.13.11.16
Gene names
Name:mhpB
Ordered Locus Names:KPN78578_20880
ORF Names:KPN_02121
OrganismKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]
Taxonomic identifier272620 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity. HAMAP MF_01653

Catalytic activity

3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP MF_01653

(2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP MF_01653

Cofactor

Fe2+ ion By similarity. HAMAP MF_01653

Pathway

Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01653

Subunit structure

Homotetramer By similarity. HAMAP MF_01653

Sequence similarities

Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   LigandIron
   Molecular functionDioxygenase
Oxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processaromatic compound catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular function3-carboxyethylcatechol 2,3-dioxygenase activity

Inferred from electronic annotation. Source: EC

ferrous iron binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3143142,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase HAMAP MF_01653
PRO_0000337652

Sites

Active site1151Proton donor By similarity
Active site1791Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
A6TAC8 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: 1B7069BDD1A9C77B

FASTA31434,404
        10         20         30         40         50         60 
MHAYLHCLSH TPLVGFVDPE QAVLDEVNRV IADARRRIAE FDPELVVLFA PDHYNGFFYD 

        70         80         90        100        110        120 
VMPPFCLGIG ATAIGDFASA AGDLPVPAEL AEACAHAILN SGIDLAVSYN MQVDHGFAQP 

       130        140        150        160        170        180 
LEFLLGGLDR VPVLPVFING VAAPLPGFQR TRLLGEAMGR FLNTLNKRVL ILGSGGLSHQ 

       190        200        210        220        230        240 
PPVPELAKAD AHLRDRLLGG GKQLPPDERE RRQQRVISAA RRFTEDPHSL HPLNPVWDNR 

       250        260        270        280        290        300 
FMSLLEQGRL SELDAIGNDE LSAMAGKSTH EIKTWVAAFA ALSAFGRWRS EGRYYRPIPE 

       310 
WIAGFGSLSA TTEI

« Hide

References

[1]The Klebsiella pneumonia Genome Sequencing Project
McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.
Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700721 / MGH 78578.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000647 Genomic DNA. Translation: ABR77549.1.
RefSeqYP_001335779.1. NC_009648.1.

3D structure databases

ProteinModelPortalA6TAC8.
ModBaseSearch...

Protein-protein interaction databases

STRINGA6TAC8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5340517.
GenomeReviewsGene locus KPN78578_20880 in contig CP000647_GR.
KEGGkpn:KPN_02121.
PATRIC20458530. VBIKlePne13394_2150.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGNOG42398.
HOGENOMHBG565586.
OMAAHEIRTW.
ProtClustDBPRK13370.

Family and domain databases

HAMAPMF_01653. MhpB.
[Tree]
InterProIPR023789. DHPP/DHXA_dioxygenase.
IPR004183. Xdiol_dOase_suB.
[Graphical view]
Gene3DG3DSA:3.40.830.10. Xdiol_dOase_3B. 2 hits.
KOK05713.
PfamPF02900. LigB. 1 hit.
[Graphical view]
SUPFAMSSF53213. Xdiol_dOase_3B. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMHPB_KLEP7
AccessionPrimary (citable) accession number: A6TAC8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 21, 2007
Last modified: January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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