Reviewed, UniProtKB/Swiss-Prot A6TAC8 (MHPB_KLEP7)

Names and origin

Protein names

Recommended name:
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
    EC=1.13.11.16

Gene names

Name:	mhpB
Ordered Locus Names:	KPN78578_20880
ORF Names:	KPN_02121

Organism

Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]

Taxonomic identifier

272620 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity.
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O(2) = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. 2,3-dihydroxicinnamic acid + O(2) = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate.
Cofactor	Fe(2+) ion By similarity.
Pathway	Aromatic compound metabolism; 3-phenylpropionic acid degradation.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydroxy-phenylpropionate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP 3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase		PRO_0000337652
Sites
	Active site	115	1	Proton donor By similarity
	Active site	179	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6TAC8-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 1B7069BDD1A9C77B	FASTA	314	34,404
	10 20 30 40 50 60 MHAYLHCLSH TPLVGFVDPE QAVLDEVNRV IADARRRIAE FDPELVVLFA PDHYNGFFYD 70 80 90 100 110 120 VMPPFCLGIG ATAIGDFASA AGDLPVPAEL AEACAHAILN SGIDLAVSYN MQVDHGFAQP 130 140 150 160 170 180 LEFLLGGLDR VPVLPVFING VAAPLPGFQR TRLLGEAMGR FLNTLNKRVL ILGSGGLSHQ 190 200 210 220 230 240 PPVPELAKAD AHLRDRLLGG GKQLPPDERE RRQQRVISAA RRFTEDPHSL HPLNPVWDNR 250 260 270 280 290 300 FMSLLEQGRL SELDAIGNDE LSAMAGKSTH EIKTWVAAFA ALSAFGRWRS EGRYYRPIPE 310 WIAGFGSLSA TTEI

References

[1]	McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000647 Genomic DNA. Translation: ABR77549.1.
RefSeq	YP_001335779.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5340517.
GenomeReviews	Gene locus KPN78578_20880 in contig CP000647_GR.
KEGG	kpn:KPN_02121.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01653. [Tree]
InterPro	IPR004183. Xdiol_dOase_3B. [Graphical view]
Gene3D	G3DSA:3.40.830.10. Xdiol_dOase_3B. 2 hits.
Pfam	PF02900. LigB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MHPB_KLEP7

Accession

Primary (citable) accession number: A6TAC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	November 25, 2008
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)