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A6TAC4 (HOA_KLEP7) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxy-2-oxovalerate aldolase

Short name=HOA
EC=4.1.3.39
Alternative name(s):
4-hydroxy-2-keto-pentanoic acid aldolase
4-hydroxy-2-oxopentanoate aldolase
Gene names
Name:mhpE
Ordered Locus Names:KPN78578_20840
ORF Names:KPN_02117
OrganismKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]
Taxonomic identifier272620 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the retro-aldol cleavage of 4-hydroxy-2-oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds By similarity. HAMAP-Rule MF_01656

Catalytic activity

(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate. HAMAP-Rule MF_01656

Pathway

Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP-Rule MF_01656

Subunit structure

Interacts with MhpF By similarity. HAMAP-Rule MF_01656

Sequence similarities

Belongs to the 4-hydroxy-2-oxovalerate aldolase family.

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   LigandManganese
Metal-binding
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_process3-phenylpropionate catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_function4-hydroxy-2-oxovalerate aldolase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3383384-hydroxy-2-oxovalerate aldolase HAMAP-Rule MF_01656
PRO_0000337807

Regions

Region14 – 152Substrate binding By similarity

Sites

Active site181Proton acceptor Potential
Metal binding151Manganese By similarity
Metal binding1971Manganese; via tele nitrogen By similarity
Metal binding1991Manganese; via tele nitrogen By similarity
Binding site1681Substrate By similarity
Binding site1971Substrate By similarity
Binding site2881Substrate By similarity
Site141Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
A6TAC4 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: FEB2817892AB3A03

FASTA33835,981
        10         20         30         40         50         60 
MNGKKLYISD VTLRDGMHAI RHQYSLAQVQ QIAGALDKAG VDSIEVAHGD GLQGSSFNYG 

        70         80         90        100        110        120 
FGAHSDIAWI EAAAAAVSQA KIATLLLPGI GTLHDLKAAY QAGARVVRVA THCSEADVAA 

       130        140        150        160        170        180 
QHIAFARELG MDTVGFLMMS HMISPQALAQ QALKMESYGA TCIYVVDSGG AMNMNDIRDR 

       190        200        210        220        230        240 
FRALKAVLKP ETATGMHAHH NLSLGVANSI VAVEEGCDRI DASLAGMGAG AGNAPLEVFI 

       250        260        270        280        290        300 
AAADKLGWQH GTDLYALMNA ADELVRPLQD RPVRVDRETL ALGYAGVYSS FLRHSEAAAK 

       310        320        330 
RYGLSAVDIL VELGKRRMVG GQEDMIVDVA LDLLNRNK 

« Hide

References

[1]The Klebsiella pneumonia Genome Sequencing Project
McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.
Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700721 / MGH 78578.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000647 Genomic DNA. Translation: ABR77545.1.
RefSeqYP_001335775.1. NC_009648.1.

3D structure databases

ProteinModelPortalA6TAC4.
SMRA6TAC4. Positions 4-338.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272620.KPN_02117.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR77545; ABR77545; KPN_02117.
GeneID5338831.
KEGGkpn:KPN_02117.
PATRIC20458522. VBIKlePne13394_2146.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0119.
HOGENOMHOG000048047.
KOK01666.
OMADIAWIEA.
OrthoDBEOG6C2WC0.
ProtClustDBCLSK2400783.

Enzyme and pathway databases

BioCycKPNE272620:GKDC-2117-MONOMER.
UniPathwayUPA00714.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01656. HOA.
InterProIPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view]
PANTHERPTHR10277:SF3. PTHR10277:SF3. 1 hit.
PfamPF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view]
ProDomPD005364. DmpG_comm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITEPS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHOA_KLEP7
AccessionPrimary (citable) accession number: A6TAC4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: August 21, 2007
Last modified: February 19, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways