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Protein

4-hydroxy-2-oxovalerate aldolase

Gene

mhpE

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the retro-aldol cleavage of 4-hydroxy-2-oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds.UniRule annotation

Catalytic activityi

(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate.UniRule annotation

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei14Transition state stabilizerUniRule annotation1
Metal bindingi15ManganeseUniRule annotation1
Active sitei18Proton acceptorUniRule annotation1
Binding sitei168SubstrateUniRule annotation1
Metal bindingi197Manganese; via tele nitrogenUniRule annotation1
Binding sitei197SubstrateUniRule annotation1
Metal bindingi199Manganese; via tele nitrogenUniRule annotation1
Binding sitei288SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-2-oxovalerate aldolaseUniRule annotation (EC:4.1.3.39UniRule annotation)
Short name:
HOAUniRule annotation
Alternative name(s):
4-hydroxy-2-keto-pentanoic acid aldolaseUniRule annotation
4-hydroxy-2-oxopentanoate aldolaseUniRule annotation
Gene namesi
Name:mhpEUniRule annotation
Ordered Locus Names:KPN78578_20840
ORF Names:KPN_02117
OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Taxonomic identifieri272620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000000265 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003378071 – 3384-hydroxy-2-oxovalerate aldolaseAdd BLAST338

Interactioni

Subunit structurei

Interacts with MhpF.UniRule annotation

Protein-protein interaction databases

STRINGi272620.KPN_02117.

Structurei

3D structure databases

ProteinModelPortaliA6TAC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 258Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST253

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 15Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the 4-hydroxy-2-oxovalerate aldolase family.UniRule annotation
Contains 1 pyruvate carboxyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105DF3. Bacteria.
COG0119. LUCA.
HOGENOMiHOG000048047.
KOiK01666.
OMAiPETATGM.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01656. HOA. 1 hit.
InterProiIPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view]
PANTHERiPTHR10277:SF3. PTHR10277:SF3. 1 hit.
PfamiPF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view]
ProDomiPD005364. DmpG_comm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITEiPS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6TAC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGKKLYISD VTLRDGMHAI RHQYSLAQVQ QIAGALDKAG VDSIEVAHGD
60 70 80 90 100
GLQGSSFNYG FGAHSDIAWI EAAAAAVSQA KIATLLLPGI GTLHDLKAAY
110 120 130 140 150
QAGARVVRVA THCSEADVAA QHIAFARELG MDTVGFLMMS HMISPQALAQ
160 170 180 190 200
QALKMESYGA TCIYVVDSGG AMNMNDIRDR FRALKAVLKP ETATGMHAHH
210 220 230 240 250
NLSLGVANSI VAVEEGCDRI DASLAGMGAG AGNAPLEVFI AAADKLGWQH
260 270 280 290 300
GTDLYALMNA ADELVRPLQD RPVRVDRETL ALGYAGVYSS FLRHSEAAAK
310 320 330
RYGLSAVDIL VELGKRRMVG GQEDMIVDVA LDLLNRNK
Length:338
Mass (Da):35,981
Last modified:August 21, 2007 - v1
Checksum:iFEB2817892AB3A03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR77545.1.
RefSeqiWP_015958581.1. NC_009648.1.

Genome annotation databases

EnsemblBacteriaiABR77545; ABR77545; KPN_02117.
KEGGikpn:KPN_02117.
PATRICi20458522. VBIKlePne13394_2146.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR77545.1.
RefSeqiWP_015958581.1. NC_009648.1.

3D structure databases

ProteinModelPortaliA6TAC4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272620.KPN_02117.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR77545; ABR77545; KPN_02117.
KEGGikpn:KPN_02117.
PATRICi20458522. VBIKlePne13394_2146.

Phylogenomic databases

eggNOGiENOG4105DF3. Bacteria.
COG0119. LUCA.
HOGENOMiHOG000048047.
KOiK01666.
OMAiPETATGM.

Enzyme and pathway databases

UniPathwayiUPA00714.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01656. HOA. 1 hit.
InterProiIPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view]
PANTHERiPTHR10277:SF3. PTHR10277:SF3. 1 hit.
PfamiPF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view]
ProDomiPD005364. DmpG_comm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITEiPS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHOA_KLEP7
AccessioniPrimary (citable) accession number: A6TAC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: August 21, 2007
Last modified: November 2, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.