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Protein

FAD-dependent urate hydroxylase

Gene

hpxO

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydroxylation of urate to 5-hydroxyisourate (HIU).1 Publication

Catalytic activityi

Urate + NADH + O2 = 5-hydroxyisourate + NAD+ + H2O.1 Publication

Cofactori

FAD1 Publication

Kineticsi

kcat is 42 sec(-1).1 Publication

Manual assertion based on experiment ini

  1. KM=42 µM for urate1 Publication

    Pathwayi: urate degradation

    This protein is involved in the pathway urate degradation, which is part of Purine metabolism.1 Publication
    View all proteins of this organism that are known to be involved in the pathway urate degradation and in Purine metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei11FAD; via amide nitrogenCombined sources1 Publication1
    Binding sitei43FADCombined sources1 Publication1
    Binding sitei125FAD; via amide nitrogen and carbonyl oxygenCombined sources1 Publication1
    Binding sitei178SubstrateCombined sources1 Publication1
    Binding sitei204SubstrateCombined sources1 Publication1
    Sitei204Involved in substrate activation for the transfer of oxygen from the flavin hydroperoxide1 Publication1
    Binding sitei285FADCombined sources1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi30 – 31FADCombined sources1 Publication2
    Nucleotide bindingi295 – 299FADCombined sources1 Publication5

    GO - Molecular functioni

    • FAD binding Source: UniProtKB
    • oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: UniProtKB
    • urate oxidase activity Source: CACAO

    GO - Biological processi

    • purine nucleobase metabolic process Source: UniProtKB-KW
    • urate catabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Purine metabolism

    Keywords - Ligandi

    FAD, Flavoprotein, NAD

    Enzyme and pathway databases

    BRENDAi1.14.13.113. 2814.
    UniPathwayiUPA00394.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    FAD-dependent urate hydroxylase1 Publication (EC:1.14.13.1131 Publication)
    Alternative name(s):
    FAD-dependent urate oxidase1 Publication
    Gene namesi
    Name:hpxO1 Publication
    Ordered Locus Names:KPN78578_16330
    ORF Names:KPN_01663
    OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
    Taxonomic identifieri272620 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella
    Proteomesi
    • UP000000265 Componenti: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi204R → Q: 160-fold decrease in catalytic activity. Results in the uncoupling of the NADH oxidation and urate hydroxylation reactions. 1 Publication1
    Mutagenesisi216Y → F: 5-fold decrease in catalytic activity. 2-fold decrease in affinity for urate and increase in affinity for NADH. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004188451 – 384FAD-dependent urate hydroxylaseAdd BLAST384

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi272620.KPN_01663.

    Structurei

    Secondary structure

    1384
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 6Combined sources5
    Helixi10 – 21Combined sources12
    Beta strandi25 – 33Combined sources9
    Beta strandi41 – 44Combined sources4
    Helixi46 – 54Combined sources9
    Helixi58 – 64Combined sources7
    Beta strandi70 – 75Combined sources6
    Turni76 – 78Combined sources3
    Beta strandi81 – 86Combined sources6
    Helixi88 – 94Combined sources7
    Beta strandi99 – 102Combined sources4
    Helixi103 – 114Combined sources12
    Helixi116 – 118Combined sources3
    Beta strandi119 – 122Combined sources4
    Beta strandi125 – 131Combined sources7
    Beta strandi134 – 139Combined sources6
    Beta strandi144 – 152Combined sources9
    Helixi161 – 165Combined sources5
    Beta strandi172 – 184Combined sources13
    Turni187 – 189Combined sources3
    Beta strandi194 – 200Combined sources7
    Beta strandi203 – 210Combined sources8
    Turni211 – 213Combined sources3
    Beta strandi214 – 222Combined sources9
    Turni231 – 233Combined sources3
    Helixi234 – 241Combined sources8
    Turni242 – 244Combined sources3
    Helixi247 – 255Combined sources9
    Helixi258 – 260Combined sources3
    Beta strandi262 – 268Combined sources7
    Beta strandi280 – 282Combined sources3
    Helixi284 – 286Combined sources3
    Helixi292 – 294Combined sources3
    Helixi297 – 313Combined sources17
    Helixi318 – 347Combined sources30
    Turni348 – 351Combined sources4
    Helixi352 – 363Combined sources12
    Helixi368 – 379Combined sources12

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3RP6X-ray2.20A1-384[»]
    3RP7X-ray2.04A1-384[»]
    3RP8X-ray1.97A1-384[»]
    ProteinModelPortaliA6T923.
    SMRiA6T923.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni216 – 218Substrate bindingCombined sources1 Publication3

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG41061H0. Bacteria.
    COG0654. LUCA.
    HOGENOMiHOG000166536.
    KOiK16839.
    OMAiYAGWNEQ.

    Family and domain databases

    Gene3Di3.50.50.60. 2 hits.
    InterProiIPR002938. FAD-bd.
    IPR023753. FAD/NAD-binding_dom.
    [Graphical view]
    PfamiPF01494. FAD_binding_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF51905. SSF51905. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    A6T923-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKAIVIGAGI GGLSAAVALK QSGIDCDVYE AVKEIKPVGA AISVWPNGVK
    60 70 80 90 100
    CMAHLGMGDI METFGGPLRR MAYRDFRSGE NMTQFSLAPL IERTGSRPCP
    110 120 130 140 150
    VSRAELQREM LDYWGRDSVQ FGKRVTRCEE DADGVTVWFT DGSSASGDLL
    160 170 180 190 200
    IAADGSHSAL RPWVLGFTPQ RRYAGYVNWN GLVEIDEALA PGDQWTTFVG
    210 220 230 240 250
    EGKRVSLMPV SAGRFYFFFD VPLPAGLAED RDTLRADLSR YFAGWAPPVQ
    260 270 280 290 300
    KLIAALDPQT TNRIEIHDIE PFSRLVRGRV ALLGDAGHST TPDIGQGGCA
    310 320 330 340 350
    AMEDAVVLGA VFRQTRDIAA ALREYEAQRC DRVRDLVLKA RKRCDITHGK
    360 370 380
    DMQLTEAWYQ ELREETGERI INGMCDTILS GPLG
    Length:384
    Mass (Da):42,107
    Last modified:August 21, 2007 - v1
    Checksum:i4B656520269C3535
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000647 Genomic DNA. Translation: ABR77094.1.
    RefSeqiWP_002904783.1. NC_009648.1.

    Genome annotation databases

    EnsemblBacteriaiABR77094; ABR77094; KPN_01663.
    KEGGikpn:KPN_01663.
    PATRICi20457612. VBIKlePne13394_1694.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000647 Genomic DNA. Translation: ABR77094.1.
    RefSeqiWP_002904783.1. NC_009648.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3RP6X-ray2.20A1-384[»]
    3RP7X-ray2.04A1-384[»]
    3RP8X-ray1.97A1-384[»]
    ProteinModelPortaliA6T923.
    SMRiA6T923.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi272620.KPN_01663.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiABR77094; ABR77094; KPN_01663.
    KEGGikpn:KPN_01663.
    PATRICi20457612. VBIKlePne13394_1694.

    Phylogenomic databases

    eggNOGiENOG41061H0. Bacteria.
    COG0654. LUCA.
    HOGENOMiHOG000166536.
    KOiK16839.
    OMAiYAGWNEQ.

    Enzyme and pathway databases

    UniPathwayiUPA00394.
    BRENDAi1.14.13.113. 2814.

    Family and domain databases

    Gene3Di3.50.50.60. 2 hits.
    InterProiIPR002938. FAD-bd.
    IPR023753. FAD/NAD-binding_dom.
    [Graphical view]
    PfamiPF01494. FAD_binding_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF51905. SSF51905. 2 hits.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiHPXO_KLEP7
    AccessioniPrimary (citable) accession number: A6T923
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: August 21, 2007
    Last modified: November 2, 2016
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.