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A6T8X0 (BGAL1_KLEP7) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase 1

Short name=Beta-gal 1
EC=3.2.1.23
Alternative name(s):
Lactase 1
Gene names
Name:lacZ1
Ordered Locus Names:KPN78578_15800
ORF Names:KPN_01610
OrganismKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]
Taxonomic identifier272620 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length1035 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10351035Beta-galactosidase 1 HAMAP-Rule MF_01687
PRO_0000367001

Regions

Region545 – 5484Substrate binding By similarity

Sites

Active site4691Proton donor By similarity
Active site5451Nucleophile By similarity
Metal binding2081Sodium By similarity
Metal binding4241Magnesium 1 By similarity
Metal binding4261Magnesium 1 By similarity
Metal binding4691Magnesium 1 By similarity
Metal binding6051Magnesium 2 By similarity
Metal binding6091Sodium; via carbonyl oxygen By similarity
Metal binding6121Sodium By similarity
Binding site1091Substrate By similarity
Binding site2081Substrate By similarity
Binding site4691Substrate By similarity
Binding site6121Substrate By similarity
Binding site10111Substrate By similarity
Site3651Transition state stabilizer By similarity
Site3991Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
A6T8X0 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: C18A68CF81E14A6A

FASTA1,035117,758
        10         20         30         40         50         60 
MQISDTGRSH TPDFHAVLAR EDWQNQTITH LNRLPAHPVF ASWRDELAAR DNLPSSRRRQ 

        70         80         90        100        110        120 
LDGEWQFSYA RSPFAVDAQW LTQDLPDCRG TPVPSNWQME GYDAPIYTNV RYPIDTTPPR 

       130        140        150        160        170        180 
VPEDNPTGCY SLHFTVEDTW RENGQTQIIF DGVNSAFHLW CNGVWVGYSQ DSRLPAAFDL 

       190        200        210        220        230        240 
SPFLRPGDNR LCVMVMRWSA GSWLEDQDMW RMSGIFRSVW LLNKPQQRLC DVQLTPALDA 

       250        260        270        280        290        300 
LYRDGTLQVQ ATIEATEAAL AGLSVGVSLW RGEEQIAAGR QPLGTPTVDE RGHYAERVDF 

       310        320        330        340        350        360 
SLAVATPAHW SAETPNCYRA VVTLWRGDEL LEAEAWDIGF RRIEIADGLL RLNGKPLLIR 

       370        380        390        400        410        420 
GVNRHEHHHL RGQVVTEADM VQDILLMKQN NFNAVRCSHY PNAPRWYELC NRYGLYVVDE 

       430        440        450        460        470        480 
ANIETHGMVP MNRLSDDPAW LPAFSARVTR MVQSNRNHPC IIIWSLGNES GGGGNHEALY 

       490        500        510        520        530        540 
HWLKRNDPSR PVQYEGGGAD TTATDIICPM YARVERDQPI PAVPKWGIKK WISLPGEQRP 

       550        560        570        580        590        600 
LILCEYAHAM GNSLGNFADY WQAFREYPRL QGGFIWDWAD QAIRKTFADG SVGWAYGGDF 

       610        620        630        640        650        660 
GDKPNDRQFC MNGLVFPDRT PHPSLVEAKH AQQYFQFTLL STSPLRVRII SEYLFRPTDN 

       670        680        690        700        710        720 
EVLRWQVQAA GEPLYHGDLT LALPPEGSDE ITLLDSLILP EGARAVWLTL EVTQPQATAW 

       730        740        750        760        770        780 
SEAEHRVAWQ QFPLPAPLAL PAPTVSAGAP DLIVSDEVWQ IRAGSQCWTI DRRTGLLSRW 

       790        800        810        820        830        840 
SVGGQEQLLT PLRDQFIRAP LDNDIGVSEV ERIDPNAWVE RWRSAGLYDL EAHCVQCDAQ 

       850        860        870        880        890        900 
RLANETLVDC RWHYLRGEEV VIVSHWRMHF TADGTLRLAV DGERAETLPP LPRVGLHFQV 

       910        920        930        940        950        960 
ADQQAPVSWL GLGPHENYPD RRSSACFARW EQPLAAMTTP YIFPTENGLR CDTQALDWGR 

       970        980        990       1000       1010       1020 
WHISGHFHFS VQPWSTRQLM ETDHWHKMQA EDGVWITLDG LHMGVGGDDS WTPSVLPQWL 

      1030 
LSQTRWHYEV SLRCL 

« Hide

References

[1]The Klebsiella pneumonia Genome Sequencing Project
McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.
Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700721 / MGH 78578.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000647 Genomic DNA. Translation: ABR77041.1.
RefSeqYP_001335271.1. NC_009648.1.

3D structure databases

ProteinModelPortalA6T8X0.
SMRA6T8X0. Positions 20-1030.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272620.KPN_01610.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR77041; ABR77041; KPN_01610.
GeneID5341643.
KEGGkpn:KPN_01610.
PATRIC20457498. VBIKlePne13394_1637.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000252443.
KOK01190.
OMAMKQANVN.
OrthoDBEOG6XWV0T.
ProtClustDBPRK09525.

Enzyme and pathway databases

BioCycKPNE272620:GKDC-1610-MONOMER.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL1_KLEP7
AccessionPrimary (citable) accession number: A6T8X0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: August 21, 2007
Last modified: February 19, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries