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A6T8X0

- BGAL1_KLEP7

UniProt

A6T8X0 - BGAL1_KLEP7

Protein

Beta-galactosidase 1

Gene

lacZ1

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091SubstrateUniRule annotation
    Metal bindingi208 – 2081SodiumUniRule annotation
    Binding sitei208 – 2081SubstrateUniRule annotation
    Sitei365 – 3651Transition state stabilizerUniRule annotation
    Sitei399 – 3991Transition state stabilizerUniRule annotation
    Metal bindingi424 – 4241Magnesium 1UniRule annotation
    Metal bindingi426 – 4261Magnesium 1UniRule annotation
    Active sitei469 – 4691Proton donorUniRule annotation
    Metal bindingi469 – 4691Magnesium 1UniRule annotation
    Binding sitei469 – 4691SubstrateUniRule annotation
    Active sitei545 – 5451NucleophileUniRule annotation
    Metal bindingi605 – 6051Magnesium 2UniRule annotation
    Metal bindingi609 – 6091Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi612 – 6121SodiumUniRule annotation
    Binding sitei612 – 6121SubstrateUniRule annotation
    Binding sitei1011 – 10111SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Enzyme and pathway databases

    BioCyciKPNE272620:GKDC-1610-MONOMER.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase 1UniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-gal 1UniRule annotation
    Alternative name(s):
    Lactase 1UniRule annotation
    Gene namesi
    Name:lacZ1UniRule annotation
    Ordered Locus Names:KPN78578_15800
    ORF Names:KPN_01610
    OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
    Taxonomic identifieri272620 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
    ProteomesiUP000000265: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10351035Beta-galactosidase 1PRO_0000367001Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272620.KPN_01610.

    Structurei

    3D structure databases

    ProteinModelPortaliA6T8X0.
    SMRiA6T8X0. Positions 20-1030.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni545 – 5484Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiQGINKPG.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6T8X0-1 [UniParc]FASTAAdd to Basket

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    MQISDTGRSH TPDFHAVLAR EDWQNQTITH LNRLPAHPVF ASWRDELAAR     50
    DNLPSSRRRQ LDGEWQFSYA RSPFAVDAQW LTQDLPDCRG TPVPSNWQME 100
    GYDAPIYTNV RYPIDTTPPR VPEDNPTGCY SLHFTVEDTW RENGQTQIIF 150
    DGVNSAFHLW CNGVWVGYSQ DSRLPAAFDL SPFLRPGDNR LCVMVMRWSA 200
    GSWLEDQDMW RMSGIFRSVW LLNKPQQRLC DVQLTPALDA LYRDGTLQVQ 250
    ATIEATEAAL AGLSVGVSLW RGEEQIAAGR QPLGTPTVDE RGHYAERVDF 300
    SLAVATPAHW SAETPNCYRA VVTLWRGDEL LEAEAWDIGF RRIEIADGLL 350
    RLNGKPLLIR GVNRHEHHHL RGQVVTEADM VQDILLMKQN NFNAVRCSHY 400
    PNAPRWYELC NRYGLYVVDE ANIETHGMVP MNRLSDDPAW LPAFSARVTR 450
    MVQSNRNHPC IIIWSLGNES GGGGNHEALY HWLKRNDPSR PVQYEGGGAD 500
    TTATDIICPM YARVERDQPI PAVPKWGIKK WISLPGEQRP LILCEYAHAM 550
    GNSLGNFADY WQAFREYPRL QGGFIWDWAD QAIRKTFADG SVGWAYGGDF 600
    GDKPNDRQFC MNGLVFPDRT PHPSLVEAKH AQQYFQFTLL STSPLRVRII 650
    SEYLFRPTDN EVLRWQVQAA GEPLYHGDLT LALPPEGSDE ITLLDSLILP 700
    EGARAVWLTL EVTQPQATAW SEAEHRVAWQ QFPLPAPLAL PAPTVSAGAP 750
    DLIVSDEVWQ IRAGSQCWTI DRRTGLLSRW SVGGQEQLLT PLRDQFIRAP 800
    LDNDIGVSEV ERIDPNAWVE RWRSAGLYDL EAHCVQCDAQ RLANETLVDC 850
    RWHYLRGEEV VIVSHWRMHF TADGTLRLAV DGERAETLPP LPRVGLHFQV 900
    ADQQAPVSWL GLGPHENYPD RRSSACFARW EQPLAAMTTP YIFPTENGLR 950
    CDTQALDWGR WHISGHFHFS VQPWSTRQLM ETDHWHKMQA EDGVWITLDG 1000
    LHMGVGGDDS WTPSVLPQWL LSQTRWHYEV SLRCL 1035
    Length:1,035
    Mass (Da):117,758
    Last modified:August 21, 2007 - v1
    Checksum:iC18A68CF81E14A6A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000647 Genomic DNA. Translation: ABR77041.1.
    RefSeqiWP_015958381.1. NC_009648.1.
    YP_001335271.1. NC_009648.1.

    Genome annotation databases

    EnsemblBacteriaiABR77041; ABR77041; KPN_01610.
    GeneIDi5341643.
    KEGGikpn:KPN_01610.
    PATRICi20457498. VBIKlePne13394_1637.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000647 Genomic DNA. Translation: ABR77041.1 .
    RefSeqi WP_015958381.1. NC_009648.1.
    YP_001335271.1. NC_009648.1.

    3D structure databases

    ProteinModelPortali A6T8X0.
    SMRi A6T8X0. Positions 20-1030.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272620.KPN_01610.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR77041 ; ABR77041 ; KPN_01610 .
    GeneIDi 5341643.
    KEGGi kpn:KPN_01610.
    PATRICi 20457498. VBIKlePne13394_1637.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi QGINKPG.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci KPNE272620:GKDC-1610-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700721 / MGH 78578.

    Entry informationi

    Entry nameiBGAL1_KLEP7
    AccessioniPrimary (citable) accession number: A6T8X0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3