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A6T8X0

- BGAL1_KLEP7

UniProt

A6T8X0 - BGAL1_KLEP7

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Protein

Beta-galactosidase 1

Gene
lacZ1, KPN78578_15800, KPN_01610
Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer By similarity.UniRule annotation
Binds 1 sodium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091Substrate By similarity
Metal bindingi208 – 2081Sodium By similarity
Binding sitei208 – 2081Substrate By similarity
Sitei365 – 3651Transition state stabilizer By similarity
Sitei399 – 3991Transition state stabilizer By similarity
Metal bindingi424 – 4241Magnesium 1 By similarity
Metal bindingi426 – 4261Magnesium 1 By similarity
Active sitei469 – 4691Proton donor By similarity
Metal bindingi469 – 4691Magnesium 1 By similarity
Binding sitei469 – 4691Substrate By similarity
Active sitei545 – 5451Nucleophile By similarity
Metal bindingi605 – 6051Magnesium 2 By similarity
Metal bindingi609 – 6091Sodium; via carbonyl oxygen By similarity
Metal bindingi612 – 6121Sodium By similarity
Binding sitei612 – 6121Substrate By similarity
Binding sitei1011 – 10111Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciKPNE272620:GKDC-1610-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 1 (EC:3.2.1.23)
Short name:
Beta-gal 1
Alternative name(s):
Lactase 1
Gene namesi
Name:lacZ1
Ordered Locus Names:KPN78578_15800
ORF Names:KPN_01610
OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Taxonomic identifieri272620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
ProteomesiUP000000265: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10351035Beta-galactosidase 1UniRule annotationPRO_0000367001Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272620.KPN_01610.

Structurei

3D structure databases

ProteinModelPortaliA6T8X0.
SMRiA6T8X0. Positions 20-1030.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni545 – 5484Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiQGINKPG.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6T8X0-1 [UniParc]FASTAAdd to Basket

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MQISDTGRSH TPDFHAVLAR EDWQNQTITH LNRLPAHPVF ASWRDELAAR     50
DNLPSSRRRQ LDGEWQFSYA RSPFAVDAQW LTQDLPDCRG TPVPSNWQME 100
GYDAPIYTNV RYPIDTTPPR VPEDNPTGCY SLHFTVEDTW RENGQTQIIF 150
DGVNSAFHLW CNGVWVGYSQ DSRLPAAFDL SPFLRPGDNR LCVMVMRWSA 200
GSWLEDQDMW RMSGIFRSVW LLNKPQQRLC DVQLTPALDA LYRDGTLQVQ 250
ATIEATEAAL AGLSVGVSLW RGEEQIAAGR QPLGTPTVDE RGHYAERVDF 300
SLAVATPAHW SAETPNCYRA VVTLWRGDEL LEAEAWDIGF RRIEIADGLL 350
RLNGKPLLIR GVNRHEHHHL RGQVVTEADM VQDILLMKQN NFNAVRCSHY 400
PNAPRWYELC NRYGLYVVDE ANIETHGMVP MNRLSDDPAW LPAFSARVTR 450
MVQSNRNHPC IIIWSLGNES GGGGNHEALY HWLKRNDPSR PVQYEGGGAD 500
TTATDIICPM YARVERDQPI PAVPKWGIKK WISLPGEQRP LILCEYAHAM 550
GNSLGNFADY WQAFREYPRL QGGFIWDWAD QAIRKTFADG SVGWAYGGDF 600
GDKPNDRQFC MNGLVFPDRT PHPSLVEAKH AQQYFQFTLL STSPLRVRII 650
SEYLFRPTDN EVLRWQVQAA GEPLYHGDLT LALPPEGSDE ITLLDSLILP 700
EGARAVWLTL EVTQPQATAW SEAEHRVAWQ QFPLPAPLAL PAPTVSAGAP 750
DLIVSDEVWQ IRAGSQCWTI DRRTGLLSRW SVGGQEQLLT PLRDQFIRAP 800
LDNDIGVSEV ERIDPNAWVE RWRSAGLYDL EAHCVQCDAQ RLANETLVDC 850
RWHYLRGEEV VIVSHWRMHF TADGTLRLAV DGERAETLPP LPRVGLHFQV 900
ADQQAPVSWL GLGPHENYPD RRSSACFARW EQPLAAMTTP YIFPTENGLR 950
CDTQALDWGR WHISGHFHFS VQPWSTRQLM ETDHWHKMQA EDGVWITLDG 1000
LHMGVGGDDS WTPSVLPQWL LSQTRWHYEV SLRCL 1035
Length:1,035
Mass (Da):117,758
Last modified:August 21, 2007 - v1
Checksum:iC18A68CF81E14A6A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000647 Genomic DNA. Translation: ABR77041.1.
RefSeqiWP_015958381.1. NC_009648.1.
YP_001335271.1. NC_009648.1.

Genome annotation databases

EnsemblBacteriaiABR77041; ABR77041; KPN_01610.
GeneIDi5341643.
KEGGikpn:KPN_01610.
PATRICi20457498. VBIKlePne13394_1637.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000647 Genomic DNA. Translation: ABR77041.1 .
RefSeqi WP_015958381.1. NC_009648.1.
YP_001335271.1. NC_009648.1.

3D structure databases

ProteinModelPortali A6T8X0.
SMRi A6T8X0. Positions 20-1030.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272620.KPN_01610.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR77041 ; ABR77041 ; KPN_01610 .
GeneIDi 5341643.
KEGGi kpn:KPN_01610.
PATRICi 20457498. VBIKlePne13394_1637.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi QGINKPG.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci KPNE272620:GKDC-1610-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700721 / MGH 78578.

Entry informationi

Entry nameiBGAL1_KLEP7
AccessioniPrimary (citable) accession number: A6T8X0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: August 21, 2007
Last modified: September 3, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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