Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-galactosidase 1

Gene

lacZ1

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109SubstrateUniRule annotation1
Metal bindingi208SodiumUniRule annotation1
Binding sitei208SubstrateUniRule annotation1
Sitei365Transition state stabilizerUniRule annotation1
Sitei399Transition state stabilizerUniRule annotation1
Metal bindingi424Magnesium 1UniRule annotation1
Metal bindingi426Magnesium 1UniRule annotation1
Active sitei469Proton donorUniRule annotation1
Metal bindingi469Magnesium 1UniRule annotation1
Binding sitei469SubstrateUniRule annotation1
Active sitei545NucleophileUniRule annotation1
Metal bindingi605Magnesium 2UniRule annotation1
Metal bindingi609Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi612SodiumUniRule annotation1
Binding sitei612SubstrateUniRule annotation1
Binding sitei1011SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 1UniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-gal 1UniRule annotation
Alternative name(s):
Lactase 1UniRule annotation
Gene namesi
Name:lacZ1UniRule annotation
Ordered Locus Names:KPN78578_15800
ORF Names:KPN_01610
OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Taxonomic identifieri272620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000000265 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003670011 – 1035Beta-galactosidase 1Add BLAST1035

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi272620.KPN_01610.

Structurei

3D structure databases

ProteinModelPortaliA6T8X0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni545 – 548Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiVASIHET.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6T8X0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQISDTGRSH TPDFHAVLAR EDWQNQTITH LNRLPAHPVF ASWRDELAAR
60 70 80 90 100
DNLPSSRRRQ LDGEWQFSYA RSPFAVDAQW LTQDLPDCRG TPVPSNWQME
110 120 130 140 150
GYDAPIYTNV RYPIDTTPPR VPEDNPTGCY SLHFTVEDTW RENGQTQIIF
160 170 180 190 200
DGVNSAFHLW CNGVWVGYSQ DSRLPAAFDL SPFLRPGDNR LCVMVMRWSA
210 220 230 240 250
GSWLEDQDMW RMSGIFRSVW LLNKPQQRLC DVQLTPALDA LYRDGTLQVQ
260 270 280 290 300
ATIEATEAAL AGLSVGVSLW RGEEQIAAGR QPLGTPTVDE RGHYAERVDF
310 320 330 340 350
SLAVATPAHW SAETPNCYRA VVTLWRGDEL LEAEAWDIGF RRIEIADGLL
360 370 380 390 400
RLNGKPLLIR GVNRHEHHHL RGQVVTEADM VQDILLMKQN NFNAVRCSHY
410 420 430 440 450
PNAPRWYELC NRYGLYVVDE ANIETHGMVP MNRLSDDPAW LPAFSARVTR
460 470 480 490 500
MVQSNRNHPC IIIWSLGNES GGGGNHEALY HWLKRNDPSR PVQYEGGGAD
510 520 530 540 550
TTATDIICPM YARVERDQPI PAVPKWGIKK WISLPGEQRP LILCEYAHAM
560 570 580 590 600
GNSLGNFADY WQAFREYPRL QGGFIWDWAD QAIRKTFADG SVGWAYGGDF
610 620 630 640 650
GDKPNDRQFC MNGLVFPDRT PHPSLVEAKH AQQYFQFTLL STSPLRVRII
660 670 680 690 700
SEYLFRPTDN EVLRWQVQAA GEPLYHGDLT LALPPEGSDE ITLLDSLILP
710 720 730 740 750
EGARAVWLTL EVTQPQATAW SEAEHRVAWQ QFPLPAPLAL PAPTVSAGAP
760 770 780 790 800
DLIVSDEVWQ IRAGSQCWTI DRRTGLLSRW SVGGQEQLLT PLRDQFIRAP
810 820 830 840 850
LDNDIGVSEV ERIDPNAWVE RWRSAGLYDL EAHCVQCDAQ RLANETLVDC
860 870 880 890 900
RWHYLRGEEV VIVSHWRMHF TADGTLRLAV DGERAETLPP LPRVGLHFQV
910 920 930 940 950
ADQQAPVSWL GLGPHENYPD RRSSACFARW EQPLAAMTTP YIFPTENGLR
960 970 980 990 1000
CDTQALDWGR WHISGHFHFS VQPWSTRQLM ETDHWHKMQA EDGVWITLDG
1010 1020 1030
LHMGVGGDDS WTPSVLPQWL LSQTRWHYEV SLRCL
Length:1,035
Mass (Da):117,758
Last modified:August 21, 2007 - v1
Checksum:iC18A68CF81E14A6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR77041.1.
RefSeqiWP_015958381.1. NC_009648.1.

Genome annotation databases

EnsemblBacteriaiABR77041; ABR77041; KPN_01610.
KEGGikpn:KPN_01610.
PATRICi20457498. VBIKlePne13394_1637.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR77041.1.
RefSeqiWP_015958381.1. NC_009648.1.

3D structure databases

ProteinModelPortaliA6T8X0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272620.KPN_01610.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR77041; ABR77041; KPN_01610.
KEGGikpn:KPN_01610.
PATRICi20457498. VBIKlePne13394_1637.

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiVASIHET.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL1_KLEP7
AccessioniPrimary (citable) accession number: A6T8X0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: August 21, 2007
Last modified: November 2, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.