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A6T8X0

- BGAL1_KLEP7

UniProt

A6T8X0 - BGAL1_KLEP7

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Protein

Beta-galactosidase 1

Gene

lacZ1

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer.UniRule annotation
Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091SubstrateUniRule annotation
Metal bindingi208 – 2081SodiumUniRule annotation
Binding sitei208 – 2081SubstrateUniRule annotation
Sitei365 – 3651Transition state stabilizerUniRule annotation
Sitei399 – 3991Transition state stabilizerUniRule annotation
Metal bindingi424 – 4241Magnesium 1UniRule annotation
Metal bindingi426 – 4261Magnesium 1UniRule annotation
Active sitei469 – 4691Proton donorUniRule annotation
Metal bindingi469 – 4691Magnesium 1UniRule annotation
Binding sitei469 – 4691SubstrateUniRule annotation
Active sitei545 – 5451NucleophileUniRule annotation
Metal bindingi605 – 6051Magnesium 2UniRule annotation
Metal bindingi609 – 6091Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi612 – 6121SodiumUniRule annotation
Binding sitei612 – 6121SubstrateUniRule annotation
Binding sitei1011 – 10111SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciKPNE272620:GKDC-1610-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 1UniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-gal 1UniRule annotation
Alternative name(s):
Lactase 1UniRule annotation
Gene namesi
Name:lacZ1UniRule annotation
Ordered Locus Names:KPN78578_15800
ORF Names:KPN_01610
OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Taxonomic identifieri272620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
ProteomesiUP000000265: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10351035Beta-galactosidase 1PRO_0000367001Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi272620.KPN_01610.

Structurei

3D structure databases

ProteinModelPortaliA6T8X0.
SMRiA6T8X0. Positions 20-1030.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni545 – 5484Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiQGINKPG.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6T8X0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQISDTGRSH TPDFHAVLAR EDWQNQTITH LNRLPAHPVF ASWRDELAAR
60 70 80 90 100
DNLPSSRRRQ LDGEWQFSYA RSPFAVDAQW LTQDLPDCRG TPVPSNWQME
110 120 130 140 150
GYDAPIYTNV RYPIDTTPPR VPEDNPTGCY SLHFTVEDTW RENGQTQIIF
160 170 180 190 200
DGVNSAFHLW CNGVWVGYSQ DSRLPAAFDL SPFLRPGDNR LCVMVMRWSA
210 220 230 240 250
GSWLEDQDMW RMSGIFRSVW LLNKPQQRLC DVQLTPALDA LYRDGTLQVQ
260 270 280 290 300
ATIEATEAAL AGLSVGVSLW RGEEQIAAGR QPLGTPTVDE RGHYAERVDF
310 320 330 340 350
SLAVATPAHW SAETPNCYRA VVTLWRGDEL LEAEAWDIGF RRIEIADGLL
360 370 380 390 400
RLNGKPLLIR GVNRHEHHHL RGQVVTEADM VQDILLMKQN NFNAVRCSHY
410 420 430 440 450
PNAPRWYELC NRYGLYVVDE ANIETHGMVP MNRLSDDPAW LPAFSARVTR
460 470 480 490 500
MVQSNRNHPC IIIWSLGNES GGGGNHEALY HWLKRNDPSR PVQYEGGGAD
510 520 530 540 550
TTATDIICPM YARVERDQPI PAVPKWGIKK WISLPGEQRP LILCEYAHAM
560 570 580 590 600
GNSLGNFADY WQAFREYPRL QGGFIWDWAD QAIRKTFADG SVGWAYGGDF
610 620 630 640 650
GDKPNDRQFC MNGLVFPDRT PHPSLVEAKH AQQYFQFTLL STSPLRVRII
660 670 680 690 700
SEYLFRPTDN EVLRWQVQAA GEPLYHGDLT LALPPEGSDE ITLLDSLILP
710 720 730 740 750
EGARAVWLTL EVTQPQATAW SEAEHRVAWQ QFPLPAPLAL PAPTVSAGAP
760 770 780 790 800
DLIVSDEVWQ IRAGSQCWTI DRRTGLLSRW SVGGQEQLLT PLRDQFIRAP
810 820 830 840 850
LDNDIGVSEV ERIDPNAWVE RWRSAGLYDL EAHCVQCDAQ RLANETLVDC
860 870 880 890 900
RWHYLRGEEV VIVSHWRMHF TADGTLRLAV DGERAETLPP LPRVGLHFQV
910 920 930 940 950
ADQQAPVSWL GLGPHENYPD RRSSACFARW EQPLAAMTTP YIFPTENGLR
960 970 980 990 1000
CDTQALDWGR WHISGHFHFS VQPWSTRQLM ETDHWHKMQA EDGVWITLDG
1010 1020 1030
LHMGVGGDDS WTPSVLPQWL LSQTRWHYEV SLRCL
Length:1,035
Mass (Da):117,758
Last modified:August 21, 2007 - v1
Checksum:iC18A68CF81E14A6A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000647 Genomic DNA. Translation: ABR77041.1.
RefSeqiWP_015958381.1. NC_009648.1.
YP_001335271.1. NC_009648.1.

Genome annotation databases

EnsemblBacteriaiABR77041; ABR77041; KPN_01610.
GeneIDi5341643.
KEGGikpn:KPN_01610.
PATRICi20457498. VBIKlePne13394_1637.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000647 Genomic DNA. Translation: ABR77041.1 .
RefSeqi WP_015958381.1. NC_009648.1.
YP_001335271.1. NC_009648.1.

3D structure databases

ProteinModelPortali A6T8X0.
SMRi A6T8X0. Positions 20-1030.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272620.KPN_01610.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR77041 ; ABR77041 ; KPN_01610 .
GeneIDi 5341643.
KEGGi kpn:KPN_01610.
PATRICi 20457498. VBIKlePne13394_1637.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi QGINKPG.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci KPNE272620:GKDC-1610-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700721 / MGH 78578.

Entry informationi

Entry nameiBGAL1_KLEP7
AccessioniPrimary (citable) accession number: A6T8X0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: August 21, 2007
Last modified: October 1, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3