Reviewed, UniProtKB/Swiss-Prot A6T7B8 (GHRA_KLEP7)

Names and origin

Protein names

Recommended name:
    Glyoxylate/hydroxypyruvate reductase A
    EC=1.1.1.79
    EC=1.1.1.81
Alternative name(s):
    2-ketoacid reductase

Gene names

Name:	ghrA
Ordered Locus Names:	KPN78578_10280
ORF Names:	KPN_01056

Organism

Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]

Taxonomic identifier

272620 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity.
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01666 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01666
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	cofactor binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: HAMAP hydroxypyruvate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Glyoxylate/hydroxypyruvate reductase A HAMAP MF_01666		PRO_0000348365
Sites
	Active site	227	1	By similarity
	Active site	275	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6T7B8-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: DF1E12624DDFE438	FASTA	312	35,187
	10 20 30 40 50 60 MEIIFYHPTF DTQYWICELE KQLPGARVRE WKAGDNRPAD YALVWHPPVE MLQGRALKAV 70 80 90 100 110 120 FALGAGVDSI LSKLRDHPDM LPLSIPLFRL EDTGMGRQMQ EYAVSQVLHW FRRFDDYQAL 130 140 150 160 170 180 KLASRWQPLP EYRADEFTVG IMGAGVLGAK VAESLQPWGF PLRVWSRSRK SWPQVQSFAG 190 200 210 220 230 240 QAELGEFMQG TRVLINLLPN TAETAGIINQ TLLAQLPDES YVLNLARGVH VVEEDLLAAL 250 260 270 280 290 300 NSGKLKGAML DVFSREPLPQ ESPLWAHPRV AMTPHVAAVT RPMEAITYIA ETISRLERGE 310 PVSGQVDRQR GY

References

[1]	McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000647 Genomic DNA. Translation: ABR76489.1.
RefSeq	YP_001334719.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6T7B8.
Genome annotation databases
GeneID	5338213.
GenomeReviews	Gene locus KPN78578_10280 in contig CP000647_GR.
KEGG	kpn:KPN_01056.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YALVWRA.
Family and domain databases
HAMAP	MF_01666. [Tree]
InterPro	IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRA_KLEP7

Accession

Primary (citable) accession number: A6T7B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)