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A6T6E8

- END8_KLEP7

UniProt

A6T6E8 - END8_KLEP7

Protein

Endonuclease 8

Gene

nei

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.UniRule annotation

    Catalytic activityi

    Removes damaged bases from DNA, leaving an abasic site.UniRule annotation
    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Schiff-base intermediate with DNAUniRule annotation
    Active sitei3 – 31Proton donorUniRule annotation
    Active sitei53 – 531Proton donor; for beta-elimination activityUniRule annotation
    Binding sitei70 – 701DNAUniRule annotation
    Binding sitei125 – 1251DNAUniRule annotation
    Binding sitei169 – 1691DNAUniRule annotation
    Active sitei253 – 2531Proton donor; for delta-elimination activityUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri229 – 26335FPG-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. damaged DNA binding Source: InterPro
    2. oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. base-excision repair Source: InterPro
    2. nucleotide-excision repair Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciKPNE272620:GKDC-726-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endonuclease 8UniRule annotation
    Alternative name(s):
    DNA glycosylase/AP lyase NeiUniRule annotation (EC:3.2.2.-UniRule annotation, EC:4.2.99.18UniRule annotation)
    DNA-(apurinic or apyrimidinic site) lyase NeiUniRule annotation
    Endonuclease VIIIUniRule annotation
    Gene namesi
    Name:neiUniRule annotation
    Ordered Locus Names:KPN78578_07080
    ORF Names:KPN_00726
    OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
    Taxonomic identifieri272620 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
    ProteomesiUP000000265: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedUniRule annotation
    Chaini2 – 263262Endonuclease 8PRO_1000067206Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272620.KPN_00726.

    Structurei

    3D structure databases

    ProteinModelPortaliA6T6E8.
    SMRiA6T6E8. Positions 2-263.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FPG family.UniRule annotation
    Contains 1 FPG-type zinc finger.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri229 – 26335FPG-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0266.
    HOGENOMiHOG000020882.
    KOiK05522.
    OMAiGPDVLDM.
    OrthoDBiEOG6QP131.

    Family and domain databases

    HAMAPiMF_01253. Endonuclease_8.
    InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR023713. Endonuclease-VIII.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PfamiPF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
    [Graphical view]
    SUPFAMiSSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    PROSITEiPS51068. FPG_CAT. 1 hit.
    PS01242. ZF_FPG_1. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A6T6E8-1 [UniParc]FASTAAdd to Basket

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    MPEGPEIRRA ADKLEAAIKG EPLTNVWFAF PQLQPYQTQL TGQRVTHIAT    50
    RGKALLTHFS GGLTLYSHNQ LYGVWRVVDA GVEPQSNRVL RVRLQTASKA 100
    ILLYSASDID ILTAEQVANH PFLLRVGPDV LDMTLTAEQV KARLLSAKFR 150
    NRQFSGLLLD QAFLAGLGNY LRVEILWQVG LSGKRKAAEL SDSQLDALAH 200
    ALLDIPRLSY RTRGLVDDNK HHGALFRFKV FHRDGERCER CGGIIEKTTL 250
    SSRPFYWCPG CQH 263
    Length:263
    Mass (Da):29,500
    Last modified:August 21, 2007 - v1
    Checksum:iF5E3F371925D0719
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000647 Genomic DNA. Translation: ABR76169.1.
    RefSeqiYP_001334399.1. NC_009648.1.

    Genome annotation databases

    EnsemblBacteriaiABR76169; ABR76169; KPN_00726.
    GeneIDi5338075.
    KEGGikpn:KPN_00726.
    PATRICi20455704. VBIKlePne13394_0750.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000647 Genomic DNA. Translation: ABR76169.1 .
    RefSeqi YP_001334399.1. NC_009648.1.

    3D structure databases

    ProteinModelPortali A6T6E8.
    SMRi A6T6E8. Positions 2-263.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272620.KPN_00726.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR76169 ; ABR76169 ; KPN_00726 .
    GeneIDi 5338075.
    KEGGi kpn:KPN_00726.
    PATRICi 20455704. VBIKlePne13394_0750.

    Phylogenomic databases

    eggNOGi COG0266.
    HOGENOMi HOG000020882.
    KOi K05522.
    OMAi GPDVLDM.
    OrthoDBi EOG6QP131.

    Enzyme and pathway databases

    BioCyci KPNE272620:GKDC-726-MONOMER.

    Family and domain databases

    HAMAPi MF_01253. Endonuclease_8.
    InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR023713. Endonuclease-VIII.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    Pfami PF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    PROSITEi PS51068. FPG_CAT. 1 hit.
    PS01242. ZF_FPG_1. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700721 / MGH 78578.

    Entry informationi

    Entry nameiEND8_KLEP7
    AccessioniPrimary (citable) accession number: A6T6E8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3