Reviewed, UniProtKB/Swiss-Prot A6T6C3 (SYQ_KLEP7)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	KPN78578_06830
ORF Names:	KPN_00701

Organism

Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) [Complete proteome] [HAMAP]

Taxonomic identifier

272620 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00126
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_1000016297
Regions
	Motif	34 – 44	11	"HIGH" region HAMAP MF_00126
	Motif	268 – 272	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6T6C3-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: C1101074BF9CBDE6	FASTA	555	63,612
	10 20 30 40 50 60 MSEAEARPTN FIRQIIDEDL ATGKHTTVHT RFPPEPNGYL HIGHAKSICL NFGIAQDYQG 70 80 90 100 110 120 QCNLRFDDTN PVKEDIEYVE SIKNDVQWLG FHWSGDVCYS SDYFDQLHQY AVELINKGLA 130 140 150 160 170 180 YVDELSPDEI REYRGTLKAP GKNSPYRDRS VEENLALFEK MRSGGFEEGK ACLRAKIDMA 190 200 210 220 230 240 SPFIVMRDPV LYRIKFAEHH QTGNKWCIYP MYDFTHCISD ALEGITHSLC TLEFQDNRRL 250 260 270 280 290 300 YDWVLDNISI PVHPRQYEFS RLNLEYTVMS KRKLNQLVTE KHVEGWDDPR MPTISGLRRR 310 320 330 340 350 360 GYTAESIREF CKRIGVTKQD NTIEMASLES CIREDLNENA PRAMAVIDPV KLVIENYPQG 370 380 390 400 410 420 ESEMVVMPNH PNKPEMGSRE VPFSAEIWID RADFREEANK QYKRLVLGKE VRLRNAYVIK 430 440 450 460 470 480 AERVEKDAEG NITTIFCTYD ADTLSKDPAD GRKVKGVIHW VSAAHALPVE IRLYDRLFSV 490 500 510 520 530 540 PNPGAADDFL AVINPESLVI KQGYAEPSLA QAEAGKAYQF EREGYFCLDS RYATATNLVF 550 NRTVGLRDTW AKAGE

References

[1]	McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P., Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000647 Genomic DNA. Translation: ABR76144.1.
RefSeq	YP_001334374.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6T6C3.
Genome annotation databases
GeneID	5338358.
GenomeReviews	Gene locus KPN78578_06830 in contig CP000647_GR.
KEGG	kpn:KPN_00701.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	RMPTIAG.
Family and domain databases
HAMAP	MF_00126. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_KLEP7

Accession

Primary (citable) accession number: A6T6C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)