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Protein

5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

Gene

mtnN

Organism
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively.UniRule annotation

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine.UniRule annotation
S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN)
  2. Methylthioribose kinase (mtnK)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei12Proton acceptorUniRule annotation1
Binding sitei78Substrate; via amide nitrogenUniRule annotation1
Binding sitei152Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei197Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00904; UER00871.

Names & Taxonomyi

Protein namesi
Recommended name:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidaseUniRule annotation (EC:3.2.2.9UniRule annotation)
Short name:
MTA/SAH nucleosidaseUniRule annotation
Short name:
MTANUniRule annotation
Alternative name(s):
5'-methylthioadenosine nucleosidaseUniRule annotation
Short name:
MTA nucleosidaseUniRule annotation
S-adenosylhomocysteine nucleosidaseUniRule annotation
Short name:
AdoHcy nucleosidaseUniRule annotation
Short name:
SAH nucleosidaseUniRule annotation
Short name:
SRH nucleosidaseUniRule annotation
Gene namesi
Name:mtnNUniRule annotation
Ordered Locus Names:KPN78578_01730
ORF Names:KPN_00174
OrganismiKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Taxonomic identifieri272620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella
Proteomesi
  • UP000000265 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003593111 – 2325'-methylthioadenosine/S-adenosylhomocysteine nucleosidaseAdd BLAST232

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272620.KPN_00174.

Structurei

Secondary structure

1232
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Helixi10 – 18Combined sources9
Beta strandi21 – 28Combined sources8
Beta strandi31 – 38Combined sources8
Beta strandi41 – 47Combined sources7
Helixi52 – 66Combined sources15
Beta strandi69 – 79Combined sources11
Beta strandi89 – 99Combined sources11
Helixi103 – 105Combined sources3
Beta strandi117 – 120Combined sources4
Helixi123 – 136Combined sources14
Beta strandi140 – 147Combined sources8
Helixi155 – 164Combined sources10
Beta strandi168 – 174Combined sources7
Helixi175 – 185Combined sources11
Beta strandi189 – 197Combined sources9
Helixi203 – 230Combined sources28

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G89X-ray2.10A/B1-232[»]
ProteinModelPortaliA6T4W3.
SMRiA6T4W3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni173 – 174Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DUF. Bacteria.
COG0775. LUCA.
HOGENOMiHOG000259346.
KOiK01243.
OMAiLLERCKP.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01684. Salvage_MtnN. 1 hit.
InterProiIPR010049. MTA_SAH_Nsdase.
IPR018017. Nucleoside_phosphorylase.
IPR000845. Nucleoside_phosphorylase_d.
[Graphical view]
PANTHERiPTHR21234. PTHR21234. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01704. MTA/SAH-Nsdase. 1 hit.

Sequencei

Sequence statusi: Complete.

A6T4W3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGIIGAME EEVTLLRDKI ENRQTITIGG SEIYTGQLHG VDVALLKSGI
60 70 80 90 100
GKVAAAMGAT LLLERCQPDV IINTGSAGGL ASTLKVGDIV VSDEARYHDA
110 120 130 140 150
DVTAFGYEYG QLPGCPAGFK ADEKLVAAAE SCIKALDLNA VRGLIVSGDA
160 170 180 190 200
FINGSVGLAK IRHNFPQAIA VEMEATAIAH VCHNFKVPFV VVRAISDVAD
210 220 230
QQSHLSFEEF LAVAARQSTL MVENLVQNLA RG
Length:232
Mass (Da):24,504
Last modified:August 21, 2007 - v1
Checksum:i53E8C78E7E7AD608
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR75634.1.
RefSeqiWP_002889282.1. NC_009648.1.

Genome annotation databases

EnsemblBacteriaiABR75634; ABR75634; KPN_00174.
KEGGikpn:KPN_00174.
PATRICi20454533. VBIKlePne13394_0181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000647 Genomic DNA. Translation: ABR75634.1.
RefSeqiWP_002889282.1. NC_009648.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G89X-ray2.10A/B1-232[»]
ProteinModelPortaliA6T4W3.
SMRiA6T4W3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272620.KPN_00174.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR75634; ABR75634; KPN_00174.
KEGGikpn:KPN_00174.
PATRICi20454533. VBIKlePne13394_0181.

Phylogenomic databases

eggNOGiENOG4105DUF. Bacteria.
COG0775. LUCA.
HOGENOMiHOG000259346.
KOiK01243.
OMAiLLERCKP.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00871.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01684. Salvage_MtnN. 1 hit.
InterProiIPR010049. MTA_SAH_Nsdase.
IPR018017. Nucleoside_phosphorylase.
IPR000845. Nucleoside_phosphorylase_d.
[Graphical view]
PANTHERiPTHR21234. PTHR21234. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01704. MTA/SAH-Nsdase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTNN_KLEP7
AccessioniPrimary (citable) accession number: A6T4W3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: August 21, 2007
Last modified: November 2, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.