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A6T345

- LIPA_JANMA

UniProt

A6T345 - LIPA_JANMA

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Protein

Lipoyl synthase

Gene
lipA, mma_3252
Organism
Janthinobacterium sp. (strain Marseille) (Minibacterium massiliensis)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi76 – 761Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi82 – 821Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi97 – 971Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi101 – 1011Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi104 – 1041Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciJSP375286:GJ8U-3298-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:mma_3252
OrganismiJanthinobacterium sp. (strain Marseille) (Minibacterium massiliensis)
Taxonomic identifieri375286 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesOxalobacteraceaeJanthinobacterium
ProteomesiUP000006388: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331Lipoyl synthaseUniRule annotationPRO_0000325266Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi375286.mma_3252.

Structurei

3D structure databases

ProteinModelPortaliA6T345.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A6T345-1 [UniParc]FASTAAdd to Basket

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MTTETNPAVT PAYNPSEKQK GAAKTIRIPI KVIPMERLPK PDWIRVKAAS    50
PSTRFYEIKD ILRANNLVTV CEEASCPNIG ECFGKGTATF MIMGDKCTRR 100
CPFCDVGHGR PDPLDVNEPE NLAKTIAALR LNYVVITSVD RDDLRDGGAG 150
HFAECIRRVR ELSPNTRIEI LVPDFRGRMD RALEILNAAP PDVMNHNLET 200
APRLYKEARP GSDYEYSLNL LKRFKAQHPN TPTKSGIMVG LGETDEEVLQ 250
VMRDMRAHDV DMLTIGQYLM PSGDHLPVRR YVHPDTFKMY EEEAYKMGFA 300
HAAVGAMVRS SYHADQQAHG VTSAQSDVVN K 331
Length:331
Mass (Da):37,041
Last modified:August 21, 2007 - v1
Checksum:i3821DD5B5BE9243B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000269 Genomic DNA. Translation: ABR89574.1.
RefSeqiWP_012081095.1. NC_009659.1.
YP_001354942.1. NC_009659.1.

Genome annotation databases

EnsemblBacteriaiABR89574; ABR89574; mma_3252.
GeneIDi5353389.
KEGGimms:mma_3252.
PATRICi22159017. VBIJanSp106498_3270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000269 Genomic DNA. Translation: ABR89574.1 .
RefSeqi WP_012081095.1. NC_009659.1.
YP_001354942.1. NC_009659.1.

3D structure databases

ProteinModelPortali A6T345.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 375286.mma_3252.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABR89574 ; ABR89574 ; mma_3252 .
GeneIDi 5353389.
KEGGi mms:mma_3252.
PATRICi 22159017. VBIJanSp106498_3270.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci JSP375286:GJ8U-3298-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome analysis of Minibacterium massiliensis highlights the convergent evolution of water-living bacteria."
    Audic S., Robert C., Campagna B., Parinello H., Claverie J.-M., Raoult D., Drancourt M.
    PLoS Genet. 3:1454-1463(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Marseille.

Entry informationi

Entry nameiLIPA_JANMA
AccessioniPrimary (citable) accession number: A6T345
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: August 21, 2007
Last modified: September 3, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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