A6T2S0 (ISPE_JANMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	mma_3127

Organism

Janthinobacterium sp. (strain Marseille) (Minibacterium massiliensis) [Complete proteome] [HAMAP]

Taxonomic identifier

375286 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Oxalobacteraceae › Janthinobacterium

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335720
Regions
	Nucleotide binding	101 – 111	11	ATP Potential
Sites
	Active site	13	1	By similarity
	Active site	143	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6T2S0 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 1F766B367A65664B	FASTA	289	31,309
	10 20 30 40 50 60 MTLTLNHCPA PAKLNLFLHV TGRRADGYHL LQSVFQLIDR GDVLHFATRD DNLIQRTTDL 70 80 90 100 110 120 PGVPAESDLV VRAAKLLQTV ATEKNPAKNF GADIAVEKKL PMGGGLGGGS SDAATTLLAL 130 140 150 160 170 180 NHLWQTGLSR AELMALGLQL GADVPFFIFG QNAFAEGIGE ALVEVETPDR WFVVIEPGVS 190 200 210 220 230 240 VPTPQIFSST ELTRDTKPVK ISDFSGAQES FGKNDLQVVA EKLFPPIAEA IQWLGQYGDA 250 260 270 280 RMTGSGACVF CPFEQEQQAD AVLTTVPPHW KAWKAKAIKH HPLAYLQQS

References

[1]

"Genome analysis of Minibacterium massiliensis highlights the convergent evolution of water-living bacteria." Audic S., Robert C., Campagna B., Parinello H., Claverie J.-M., Raoult D., Drancourt M. PLoS Genet. 3:1454-1463(2007) [PubMed: 17722982] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Marseille.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000269 Genomic DNA. Translation: ABR88561.1.
RefSeq	YP_001354817.1. NC_009659.1.
3D structure databases
ProteinModelPortal	A6T2S0.
ModBase	Search...
Protein-protein interaction databases
STRING	A6T2S0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5353294.
GenomeReviews	Gene locus mma_3127 in contig CP000269_GR.
KEGG	mms:mma_3127.
PATRIC	22158757. VBIJanSp106498_3141.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	LNLFLHV.
ProtClustDB	PRK00343.
Enzyme and pathway databases
BioCyc	JSP375286:MMA_3127-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_JANMA

Accession

Primary (citable) accession number: A6T2S0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program