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Reviewed, UniProtKB/Swiss-Prot A6T2G3 (MURE_JANMA)

Last modified November 3, 2009. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase
Gene names
Name: murE
Ordered Locus Names: mma_3020
OrganismJanthinobacterium sp. (strain Marseille) (Minibacterium massiliensis) [Complete proteome] [HAMAP]
Taxonomic identifier375286 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Oxalobacteraceae › Janthinobacterium

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Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208

Subcellular location

Cytoplasm By similarity.

Post-translational modification

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.

Sequence similarities

Belongs to the murCDEF family. MurE subfamily.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 508508UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208
PRO_1000012360

Regions

Nucleotide binding112 – 1187ATP Potential
Region159 – 1602UDP-MurNAc-L-Ala-D-Glu binding By similarity
Region421 – 4244Meso-diaminopimelate binding By similarity
Motif421 – 4244Meso-diaminopimelate recognition motif HAMAP MF_00208

Sites

Binding site291UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1861UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1921UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1941UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site3981Meso-diaminopimelate By similarity
Binding site4731Meso-diaminopimelate; via carbonyl oxygen By similarity
Binding site4771Meso-diaminopimelate By similarity

Amino acid modifications

Modified residue2261N6-carboxylysine By similarity

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Sequences

Sequence LengthMass (Da)Tools
A6T2G3-1 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: C9A24F5BB15E7727

FASTA50853,759
        10         20         30         40         50         60 
MSILTTTALA SILDWLAANA PKAELASDSR RIAQGDVFVA YPGDEADGRS YIANAIERGA 

        70         80         90        100        110        120 
QAVIYEAAGC TWDAEWDVAH LAIDDLKEHA GEIAAAYYAH PDRSMFTVAI TGTNGKTSCA 

       130        140        150        160        170        180 
QWLGSALSRL GQPTAVIGTL GVGIFTNGGH GSFDVTGYTT PDAVLLQRSL VNVSNLGATA 

       190        200        210        220        230        240 
LAIEASSIGL HQGRLSGMHF DMALFTNFTR DHLDYHGDMA AYEEAKAMLF DWPGLQHAVI 

       250        260        270        280        290        300 
NLDDAMGVRL VQRLQSRQAD VGITGYTLSD KKIDGIAVLR ATDIRSNQSG TVFQLESSAG 

       310        320        330        340        350        360 
NTQVKTQLVG QFNVSNVLGI IGILLAKGIA LQDAVNAVEA LTAVPGRMQQ LGGGEAPLVV 

       370        380        390        400        410        420 
IDYAHTPDAL EKTLATLRSV ANDRGGELWC VFGCGGDRDP GKRPQMGKVS MAADHIVVTT 

       430        440        450        460        470        480 
DNPRNEEPAN IIGDIVAGIT APKNAPQIIE DRASAILWAG RHAARQDVIL LAGKGHEAYQ 

       490        500 
EVKGRKLPFL DADHAALALS TRVMQGAS

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References

[1]"Genome analysis of Minibacterium massiliensis highlights the convergent evolution of water-living bacteria."
Audic S., Robert C., Campagna B., Parinello H., Claverie J.-M., Raoult D., Drancourt M.
PLoS Genet. 3:1454-1463(2007) [PubMed: 17722982] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000269 Genomic DNA. Translation: ABR89221.1.
RefSeqYP_001354710.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGA6T2G3.

Genome annotation databases

GeneID5350421.
GenomeReviewsGene locus mma_3020 in contig CP000269_GR.
KEGGmms:mma_3020.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAFPVIVDY.

Family and domain databases

HAMAPMF_00208.
[Tree]
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
PfamPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
TIGRFAMsTIGR01085. murE. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameMURE_JANMA
AccessionPrimary (citable) accession number: A6T2G3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents