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A6T0G1 (A6T0G1_JANMA) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Adenylosuccinate synthetase 2 HAMAP MF_00011
Short name=AMPSase 2 HAMAP MF_00011
Short name=AdSS 2 HAMAP MF_00011
EC=6.3.4.4 HAMAP MF_00011
Alternative name(s):
IMP--aspartate ligase 2 HAMAP MF_00011
Gene names
Name:purA2 HAMAP MF_00011 EMBL ABR89324.1
Ordered Locus Names:mma_2318
OrganismJanthinobacterium sp. (strain Marseille) (Minibacterium massiliensis) [Complete proteome] [HAMAP]
Taxonomic identifier375286 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Oxalobacteraceae › Janthinobacterium

Protein attributes

Sequence length455 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011

Catalytic activity

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011

Pathway

Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011

Subunit structure

Homodimer By similarity. HAMAP MF_00011

Subcellular location

Cytoplasm By similarity HAMAP MF_00011.

Sequence similarities

Belongs to the adenylosuccinate synthetase family. HAMAP MF_00011

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding17 – 237GTP By similarity HAMAP MF_00011
Nucleotide binding45 – 473GTP By similarity HAMAP MF_00011
Nucleotide binding372 – 3743GTP By similarity HAMAP MF_00011
Nucleotide binding435 – 4373GTP By similarity HAMAP MF_00011
Region18 – 214IMP binding By similarity HAMAP MF_00011
Region43 – 464IMP binding By similarity HAMAP MF_00011
Region340 – 3467Substrate binding By similarity HAMAP MF_00011

Sites

Active site181Proton acceptor By similarity HAMAP MF_00011
Active site461Proton donor By similarity HAMAP MF_00011
Metal binding181Magnesium By similarity HAMAP MF_00011
Metal binding451Magnesium; via carbonyl oxygen By similarity HAMAP MF_00011
Binding site1291IMP By similarity HAMAP MF_00011
Binding site1431IMP; shared with dimeric partner By similarity HAMAP MF_00011
Binding site2291IMP By similarity HAMAP MF_00011
Binding site2441IMP By similarity HAMAP MF_00011
Binding site3441IMP By similarity HAMAP MF_00011
Binding site3461GTP By similarity HAMAP MF_00011

Sequences

Sequence LengthMass (Da)Tools
A6T0G1 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: AE7CB84E19F8FE9C

FASTA45549,249
        10         20         30         40         50         60 
MMKAAGYADV LVGLQYGDEG KAKIVDLLAK GYDIVARFNG GANAGHTVVT RQGTVKLRQI 

        70         80         90        100        110        120 
PSAVFYPDKL LYIGSGCAVS VVQLAEEIRS LEELGISLRG RLYVSSRCVL VQPSHIHLDQ 

       130        140        150        160        170        180 
MHGKEIGTTG NGIGPCYADR ALRMSDGRCI SLQIRDLLGD SAAAFEMMHE RYAAQLGLHE 

       190        200        210        220        230        240 
LHAEKSHVDA SMQALQEAWA VVKAYVTDDP LFLVKRVDAG AHVLFEGAQS VLLDVMYGDQ 

       250        260        270        280        290        300 
PYVTSSHTAP SYAYVGGDLP CRYHRKSIGV AKAVMSRVGS GAFPSELGAS RSEQYCRHAA 

       310        320        330        340        350        360 
QNGIGAASEK AGFDALELLR SDDAFKQGIA LRMLTGEYGT GSGRPRRVGL LDIGQLADVV 

       370        380        390        400        410        420 
ALHGIDEIFL NKCDCLAYFS YTPQECIPMF AGKGGDRSMV NFPAFTLEGK EIPFELLQIL 

       430        440        450 
QFIEEQTACR IRGVGIGPER DQSVFFNETA LIGAQ

« Hide

References

[1]"Genome analysis of Minibacterium massiliensis highlights the convergent evolution of water-living bacteria."
Audic S., Robert C., Campagna B., Parinello H., Claverie J.-M., Raoult D., Drancourt M.
PLoS Genet. 3:1454-1463(2007) [PubMed: 17722982] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000269 Genomic DNA. Translation: ABR89324.1.
RefSeqYP_001354008.1. NC_009659.1.

3D structure databases

ProteinModelPortalA6T0G1.
ModBaseSearch...

Protein-protein interaction databases

STRINGA6T0G1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5351118.
GenomeReviewsGene locus mma_2318 in contig CP000269_GR.
KEGGmms:mma_2318.
PATRIC22157123. VBIJanSp106498_2327.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0104.
HOGENOMHBG658237.
OMALFEGAQS.
ProtClustDBCLSK939171.

Family and domain databases

HAMAPMF_00011. Adenylosucc_synth.
[Tree]
InterProIPR001114. Adenylosuccinate_synthetase.
[Graphical view]
KOK01939.
PANTHERPTHR11846. Asucc_synthtase. 1 hit.
PfamPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA6T0G1_JANMA
AccessionPrimary (citable) accession number: A6T0G1
Entry history
Integrated into UniProtKB/TrEMBL: August 21, 2007
Last sequence update: August 21, 2007
Last modified: December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info