A6SZP1 (LPXD_JANMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	mma_2048

Organism

Janthinobacterium sp. (strain Marseille) (Minibacterium massiliensis) [Complete proteome] [HAMAP]

Taxonomic identifier

375286 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Oxalobacteraceae › Janthinobacterium

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_1000050944
Sites
	Active site	244	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6SZP1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: E5EE6580B29FB953	FASTA	350	36,326
	10 20 30 40 50 60 MSTRLGELVE RLGGRLIGDA DIEVVGIAPL NDADASHITF LSNPKFRGQA AQTHAAALIL 70 80 90 100 110 120 SAADDEVVAA DYQGARIVTA NPYAYFARAA QFFAALHAHI APAGIHPSAS VDPTAQIAAS 130 140 150 160 170 180 ASIGPFVAVE AGAVIEDGCV IDAGCFIGRD ARVGSGTHFY PRVTFLAGCR IGARGIIHSG 190 200 210 220 230 240 AVIGADGFGF ANEGGVYIKI PQTGAVRIGD DVEIGANTSI DRGALADTVL EDGVKLDNQI 250 260 270 280 290 300 QIGHNCHIGA HTAMAGCVGV AGSAIIGKYC TFGGAAMVLG HLTIADHVHI SSGSMVSRSI 310 320 330 340 350 REPGQYTGFY PLAKNAEWEK SAVIVRNLAT MREKIRELEK TIKSLGDKPE

References

[1]

"Genome analysis of Minibacterium massiliensis highlights the convergent evolution of water-living bacteria." Audic S., Robert C., Campagna B., Parinello H., Claverie J.-M., Raoult D., Drancourt M. PLoS Genet. 3:1454-1463(2007) [PubMed: 17722982] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Marseille.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000269 Genomic DNA. Translation: ABR88639.1.
RefSeq	YP_001353738.1. NC_009659.1.
3D structure databases
ProteinModelPortal	A6SZP1.
ModBase	Search...
Protein-protein interaction databases
STRING	A6SZP1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5350775.
GenomeReviews	Gene locus mma_2048 in contig CP000269_GR.
KEGG	mms:mma_2048.
PATRIC	22156595. VBIJanSp106498_2067.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	CFVGKNT.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	JSP375286:MMA_2048-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_JANMA

Accession

Primary (citable) accession number: A6SZP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program