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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Janthinobacterium sp. (strain Marseille) (Minibacterium massiliensis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei590 – 5901Aminoacyl-adenylateUniRule annotation
Binding sitei634 – 6341ATPUniRule annotation
Metal bindingi921 – 9211ZincUniRule annotation
Metal bindingi924 – 9241ZincUniRule annotation
Metal bindingi941 – 9411ZincUniRule annotation
Metal bindingi944 – 9441ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciJSP375286:GJ8U-845-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:mma_0828
OrganismiJanthinobacterium sp. (strain Marseille) (Minibacterium massiliensis)
Taxonomic identifieri375286 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesOxalobacteraceaeJanthinobacterium
ProteomesiUP000006388 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 958958Isoleucine--tRNA ligasePRO_1000022080Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi375286.mma_0828.

Structurei

3D structure databases

ProteinModelPortaliA6SW71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi71 – 8111"HIGH" regionAdd
BLAST
Motifi631 – 6355"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6SW71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDNQNKPGK PAAKPQSKYP VNMTDTPFPM RGDLAKREPQ WVKQWQDQKV
60 70 80 90 100
YEKIRKASKG RPKFILHDGP PYANGDIHLG HAVNKILKDM IVKTRQFDGF
110 120 130 140 150
DAPYVPGWDC HGMPIEIQIE KQFGKNLPTA EVLSKARAYA NEQVERQKKD
160 170 180 190 200
FIRLGVLGQW DKPYKTMDFG NEADELRALG SLLEKGYVYR GLKPVNWCFD
210 220 230 240 250
CGSALAEAEV EYQDKRDPAI DVGFPFAEPE KVAKAFGLAK LPTNKGYAII
260 270 280 290 300
WTTTPWTIPA NQALNVHGDL PYALVNVVRN GVPQLLILAA DLVGTVLQRC
310 320 330 340 350
GLTGETIAIC EGSALEGIRF KHPFADLDPG YNRESPIYLA TYVAADSGTG
360 370 380 390 400
IVHSAPAYGI EDFISCKSHG MRDDEIIAPV MGDGKYASWL PLFGGLTIWE
410 420 430 440 450
ASKPICAKLD EVGSLFKLVM FDHSYMHCWR HKTPIIYRAT SQWFAGMDVM
460 470 480 490 500
PKNQGATLRE TALQAIEETE FFPSWGKARL HGMIANRPDW TLSRQRQWGV
510 520 530 540 550
PMAFFVHKET GDLHPRTPEL LEQIAQRVEK DGIEAWLTLD PKELLGADAD
560 570 580 590 600
MYLKNKDTLD VWFDSGCTHQ TVLRGSHKEE LAFPADLYLE GSDQHRGWFH
610 620 630 640 650
SSLLTSSMMN GRAPYKALLT HGFTVDGEGK KMSKSLGNTL APQKISDTLG
660 670 680 690 700
ADILRLWIAS TDYSGELSIS DEILKRVTES YRRIRNTVRF LLSNTSDFDA
710 720 730 740 750
AKDLVPVADM LEIDRYAVAQ MNAMQAEILA HYKVYEFHPV VSKLQMYCSE
760 770 780 790 800
DLGGFYLDIL KDRLYTSGVT SHARRSAQSA IWHLTHSLLR LMAPILSFTA
810 820 830 840 850
EEAWAVFASP DVNTDGTIFT HTFYQLPEVS DGAALLAKYT LLREVRNDVT
860 870 880 890 900
KQLEEVRVAG GIGSSLQAEV ELKASGDKFA ALASLDDDLK FVLITSQAGV
910 920 930 940 950
SQVASAEEES VVVTPSTYQK CERCWHYRAD VGSHAEHEGL CGRCVANLFG

KGEARRFA
Length:958
Mass (Da):106,938
Last modified:August 21, 2007 - v1
Checksum:iB78F1F213C8C815A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000269 Genomic DNA. Translation: ABR88910.1.
RefSeqiWP_012078692.1. NC_009659.1.

Genome annotation databases

EnsemblBacteriaiABR88910; ABR88910; mma_0828.
KEGGimms:mma_0828.
PATRICi22154131. VBIJanSp106498_0854.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000269 Genomic DNA. Translation: ABR88910.1.
RefSeqiWP_012078692.1. NC_009659.1.

3D structure databases

ProteinModelPortaliA6SW71.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi375286.mma_0828.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR88910; ABR88910; mma_0828.
KEGGimms:mma_0828.
PATRICi22154131. VBIJanSp106498_0854.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciJSP375286:GJ8U-845-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome analysis of Minibacterium massiliensis highlights the convergent evolution of water-living bacteria."
    Audic S., Robert C., Campagna B., Parinello H., Claverie J.-M., Raoult D., Drancourt M.
    PLoS Genet. 3:1454-1463(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Marseille.

Entry informationi

Entry nameiSYI_JANMA
AccessioniPrimary (citable) accession number: A6SW71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: July 22, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.