Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A6SW71 (SYI_JANMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:mma_0828
OrganismJanthinobacterium sp. (strain Marseille) (Minibacterium massiliensis) [Complete proteome] [HAMAP]
Taxonomic identifier375286 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesOxalobacteraceaeJanthinobacterium

Protein attributes

Sequence length958 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 958958Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_1000022080

Regions

Motif71 – 8111"HIGH" region HAMAP-Rule MF_02002
Motif631 – 6355"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding9211Zinc By similarity
Metal binding9241Zinc By similarity
Metal binding9411Zinc By similarity
Metal binding9441Zinc By similarity
Binding site5901Aminoacyl-adenylate By similarity
Binding site6341ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A6SW71 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: B78F1F213C8C815A

FASTA958106,938
        10         20         30         40         50         60 
MSDNQNKPGK PAAKPQSKYP VNMTDTPFPM RGDLAKREPQ WVKQWQDQKV YEKIRKASKG 

        70         80         90        100        110        120 
RPKFILHDGP PYANGDIHLG HAVNKILKDM IVKTRQFDGF DAPYVPGWDC HGMPIEIQIE 

       130        140        150        160        170        180 
KQFGKNLPTA EVLSKARAYA NEQVERQKKD FIRLGVLGQW DKPYKTMDFG NEADELRALG 

       190        200        210        220        230        240 
SLLEKGYVYR GLKPVNWCFD CGSALAEAEV EYQDKRDPAI DVGFPFAEPE KVAKAFGLAK 

       250        260        270        280        290        300 
LPTNKGYAII WTTTPWTIPA NQALNVHGDL PYALVNVVRN GVPQLLILAA DLVGTVLQRC 

       310        320        330        340        350        360 
GLTGETIAIC EGSALEGIRF KHPFADLDPG YNRESPIYLA TYVAADSGTG IVHSAPAYGI 

       370        380        390        400        410        420 
EDFISCKSHG MRDDEIIAPV MGDGKYASWL PLFGGLTIWE ASKPICAKLD EVGSLFKLVM 

       430        440        450        460        470        480 
FDHSYMHCWR HKTPIIYRAT SQWFAGMDVM PKNQGATLRE TALQAIEETE FFPSWGKARL 

       490        500        510        520        530        540 
HGMIANRPDW TLSRQRQWGV PMAFFVHKET GDLHPRTPEL LEQIAQRVEK DGIEAWLTLD 

       550        560        570        580        590        600 
PKELLGADAD MYLKNKDTLD VWFDSGCTHQ TVLRGSHKEE LAFPADLYLE GSDQHRGWFH 

       610        620        630        640        650        660 
SSLLTSSMMN GRAPYKALLT HGFTVDGEGK KMSKSLGNTL APQKISDTLG ADILRLWIAS 

       670        680        690        700        710        720 
TDYSGELSIS DEILKRVTES YRRIRNTVRF LLSNTSDFDA AKDLVPVADM LEIDRYAVAQ 

       730        740        750        760        770        780 
MNAMQAEILA HYKVYEFHPV VSKLQMYCSE DLGGFYLDIL KDRLYTSGVT SHARRSAQSA 

       790        800        810        820        830        840 
IWHLTHSLLR LMAPILSFTA EEAWAVFASP DVNTDGTIFT HTFYQLPEVS DGAALLAKYT 

       850        860        870        880        890        900 
LLREVRNDVT KQLEEVRVAG GIGSSLQAEV ELKASGDKFA ALASLDDDLK FVLITSQAGV 

       910        920        930        940        950 
SQVASAEEES VVVTPSTYQK CERCWHYRAD VGSHAEHEGL CGRCVANLFG KGEARRFA 

« Hide

References

[1]"Genome analysis of Minibacterium massiliensis highlights the convergent evolution of water-living bacteria."
Audic S., Robert C., Campagna B., Parinello H., Claverie J.-M., Raoult D., Drancourt M.
PLoS Genet. 3:1454-1463(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Marseille.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000269 Genomic DNA. Translation: ABR88910.1.
RefSeqYP_001352518.1. NC_009659.1.

3D structure databases

ProteinModelPortalA6SW71.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING375286.mma_0828.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR88910; ABR88910; mma_0828.
GeneID5352941.
KEGGmms:mma_0828.
PATRIC22154131. VBIJanSp106498_0854.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAKPVHWCL.
OrthoDBEOG644ZM1.

Enzyme and pathway databases

BioCycJSP375286:GJ8U-845-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_JANMA
AccessionPrimary (citable) accession number: A6SW71
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries