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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Janthinobacterium sp. (strain Marseille) (Minibacterium massiliensis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei590Aminoacyl-adenylateUniRule annotation1
Binding sitei634ATPUniRule annotation1
Metal bindingi921ZincUniRule annotation1
Metal bindingi924ZincUniRule annotation1
Metal bindingi941ZincUniRule annotation1
Metal bindingi944ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciJSP375286:G1G65-866-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:mma_0828
OrganismiJanthinobacterium sp. (strain Marseille) (Minibacterium massiliensis)
Taxonomic identifieri375286 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesOxalobacteraceaeJanthinobacterium
Proteomesi
  • UP000006388 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000220801 – 958Isoleucine--tRNA ligaseAdd BLAST958

Proteomic databases

PRIDEiA6SW71

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi375286.mma_0828

Structurei

3D structure databases

ProteinModelPortaliA6SW71
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi71 – 81"HIGH" regionAdd BLAST11
Motifi631 – 635"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A6SW71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDNQNKPGK PAAKPQSKYP VNMTDTPFPM RGDLAKREPQ WVKQWQDQKV
60 70 80 90 100
YEKIRKASKG RPKFILHDGP PYANGDIHLG HAVNKILKDM IVKTRQFDGF
110 120 130 140 150
DAPYVPGWDC HGMPIEIQIE KQFGKNLPTA EVLSKARAYA NEQVERQKKD
160 170 180 190 200
FIRLGVLGQW DKPYKTMDFG NEADELRALG SLLEKGYVYR GLKPVNWCFD
210 220 230 240 250
CGSALAEAEV EYQDKRDPAI DVGFPFAEPE KVAKAFGLAK LPTNKGYAII
260 270 280 290 300
WTTTPWTIPA NQALNVHGDL PYALVNVVRN GVPQLLILAA DLVGTVLQRC
310 320 330 340 350
GLTGETIAIC EGSALEGIRF KHPFADLDPG YNRESPIYLA TYVAADSGTG
360 370 380 390 400
IVHSAPAYGI EDFISCKSHG MRDDEIIAPV MGDGKYASWL PLFGGLTIWE
410 420 430 440 450
ASKPICAKLD EVGSLFKLVM FDHSYMHCWR HKTPIIYRAT SQWFAGMDVM
460 470 480 490 500
PKNQGATLRE TALQAIEETE FFPSWGKARL HGMIANRPDW TLSRQRQWGV
510 520 530 540 550
PMAFFVHKET GDLHPRTPEL LEQIAQRVEK DGIEAWLTLD PKELLGADAD
560 570 580 590 600
MYLKNKDTLD VWFDSGCTHQ TVLRGSHKEE LAFPADLYLE GSDQHRGWFH
610 620 630 640 650
SSLLTSSMMN GRAPYKALLT HGFTVDGEGK KMSKSLGNTL APQKISDTLG
660 670 680 690 700
ADILRLWIAS TDYSGELSIS DEILKRVTES YRRIRNTVRF LLSNTSDFDA
710 720 730 740 750
AKDLVPVADM LEIDRYAVAQ MNAMQAEILA HYKVYEFHPV VSKLQMYCSE
760 770 780 790 800
DLGGFYLDIL KDRLYTSGVT SHARRSAQSA IWHLTHSLLR LMAPILSFTA
810 820 830 840 850
EEAWAVFASP DVNTDGTIFT HTFYQLPEVS DGAALLAKYT LLREVRNDVT
860 870 880 890 900
KQLEEVRVAG GIGSSLQAEV ELKASGDKFA ALASLDDDLK FVLITSQAGV
910 920 930 940 950
SQVASAEEES VVVTPSTYQK CERCWHYRAD VGSHAEHEGL CGRCVANLFG

KGEARRFA
Length:958
Mass (Da):106,938
Last modified:August 21, 2007 - v1
Checksum:iB78F1F213C8C815A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000269 Genomic DNA Translation: ABR88910.1
RefSeqiWP_012078692.1, NC_009659.1

Genome annotation databases

EnsemblBacteriaiABR88910; ABR88910; mma_0828
KEGGimms:mma_0828

Similar proteinsi

Entry informationi

Entry nameiSYI_JANMA
AccessioniPrimary (citable) accession number: A6SW71
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 21, 2007
Last modified: May 23, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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