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A6SW71

- SYI_JANMA

UniProt

A6SW71 - SYI_JANMA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Janthinobacterium sp. (strain Marseille) (Minibacterium massiliensis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei590 – 5901Aminoacyl-adenylateUniRule annotation
    Binding sitei634 – 6341ATPUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation
    Metal bindingi924 – 9241ZincUniRule annotation
    Metal bindingi941 – 9411ZincUniRule annotation
    Metal bindingi944 – 9441ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciJSP375286:GJ8U-845-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:mma_0828
    OrganismiJanthinobacterium sp. (strain Marseille) (Minibacterium massiliensis)
    Taxonomic identifieri375286 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesOxalobacteraceaeJanthinobacterium
    ProteomesiUP000006388: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 958958Isoleucine--tRNA ligasePRO_1000022080Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi375286.mma_0828.

    Structurei

    3D structure databases

    ProteinModelPortaliA6SW71.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi71 – 8111"HIGH" regionAdd
    BLAST
    Motifi631 – 6355"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6SW71-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDNQNKPGK PAAKPQSKYP VNMTDTPFPM RGDLAKREPQ WVKQWQDQKV    50
    YEKIRKASKG RPKFILHDGP PYANGDIHLG HAVNKILKDM IVKTRQFDGF 100
    DAPYVPGWDC HGMPIEIQIE KQFGKNLPTA EVLSKARAYA NEQVERQKKD 150
    FIRLGVLGQW DKPYKTMDFG NEADELRALG SLLEKGYVYR GLKPVNWCFD 200
    CGSALAEAEV EYQDKRDPAI DVGFPFAEPE KVAKAFGLAK LPTNKGYAII 250
    WTTTPWTIPA NQALNVHGDL PYALVNVVRN GVPQLLILAA DLVGTVLQRC 300
    GLTGETIAIC EGSALEGIRF KHPFADLDPG YNRESPIYLA TYVAADSGTG 350
    IVHSAPAYGI EDFISCKSHG MRDDEIIAPV MGDGKYASWL PLFGGLTIWE 400
    ASKPICAKLD EVGSLFKLVM FDHSYMHCWR HKTPIIYRAT SQWFAGMDVM 450
    PKNQGATLRE TALQAIEETE FFPSWGKARL HGMIANRPDW TLSRQRQWGV 500
    PMAFFVHKET GDLHPRTPEL LEQIAQRVEK DGIEAWLTLD PKELLGADAD 550
    MYLKNKDTLD VWFDSGCTHQ TVLRGSHKEE LAFPADLYLE GSDQHRGWFH 600
    SSLLTSSMMN GRAPYKALLT HGFTVDGEGK KMSKSLGNTL APQKISDTLG 650
    ADILRLWIAS TDYSGELSIS DEILKRVTES YRRIRNTVRF LLSNTSDFDA 700
    AKDLVPVADM LEIDRYAVAQ MNAMQAEILA HYKVYEFHPV VSKLQMYCSE 750
    DLGGFYLDIL KDRLYTSGVT SHARRSAQSA IWHLTHSLLR LMAPILSFTA 800
    EEAWAVFASP DVNTDGTIFT HTFYQLPEVS DGAALLAKYT LLREVRNDVT 850
    KQLEEVRVAG GIGSSLQAEV ELKASGDKFA ALASLDDDLK FVLITSQAGV 900
    SQVASAEEES VVVTPSTYQK CERCWHYRAD VGSHAEHEGL CGRCVANLFG 950
    KGEARRFA 958
    Length:958
    Mass (Da):106,938
    Last modified:August 21, 2007 - v1
    Checksum:iB78F1F213C8C815A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000269 Genomic DNA. Translation: ABR88910.1.
    RefSeqiWP_012078692.1. NC_009659.1.
    YP_001352518.1. NC_009659.1.

    Genome annotation databases

    EnsemblBacteriaiABR88910; ABR88910; mma_0828.
    GeneIDi5352941.
    KEGGimms:mma_0828.
    PATRICi22154131. VBIJanSp106498_0854.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000269 Genomic DNA. Translation: ABR88910.1 .
    RefSeqi WP_012078692.1. NC_009659.1.
    YP_001352518.1. NC_009659.1.

    3D structure databases

    ProteinModelPortali A6SW71.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 375286.mma_0828.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR88910 ; ABR88910 ; mma_0828 .
    GeneIDi 5352941.
    KEGGi mms:mma_0828.
    PATRICi 22154131. VBIJanSp106498_0854.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci JSP375286:GJ8U-845-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome analysis of Minibacterium massiliensis highlights the convergent evolution of water-living bacteria."
      Audic S., Robert C., Campagna B., Parinello H., Claverie J.-M., Raoult D., Drancourt M.
      PLoS Genet. 3:1454-1463(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Marseille.

    Entry informationi

    Entry nameiSYI_JANMA
    AccessioniPrimary (citable) accession number: A6SW71
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3