A6SHZ5 (PMIP_BOTFB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial intermediate peptidase
Short name=MIP
EC=3.4.24.59

Alternative name(s):
Octapeptidyl aminopeptidase

Gene names

Name:	oct1
ORF Names:	BC1G_12067

Organism

Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)

Taxonomic identifier

332648 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Leotiomycetes › Helotiales › Sclerotiniaceae › Botryotinia › Botryotinia fuckeliana

Protein attributes

Sequence length	762 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.
Sequence caution	The sequence EDN33764.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Mitochondrion
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 762	›762	Mitochondrial intermediate peptidase		PRO_0000338575
Regions
	Compositional bias	260 – 264	5	Poly-Ala
Sites
	Active site	544	1	By similarity
	Metal binding	543	1	Zinc; catalytic By similarity
	Metal binding	547	1	Zinc; catalytic By similarity
	Metal binding	550	1	Zinc; catalytic By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	A6SHZ5 [UniParc]. Last modified June 10, 2008. Version 2. Checksum: 383090D54BF577B9	FASTA	762	85,181
	10 20 30 40 50 60 RRLATASTQY RESRPVPVDN SAPGAKRDDR TLRQIFDSPN FWAEFSQSSK QSYNRPAVGL 70 80 90 100 110 120 FQNRYLVNPQ GFEVFANTSL RKAQRIVDKV LSASTVEEYR HVARELDRLS DLLCRVIDLS 130 140 150 160 170 180 DFVRATHPNA AIQAAASRAY AKMFEYMNIL NTTTGLDKQL EIAMATPEIV AGWTEEEVVV 190 200 210 220 230 240 ADILRKDFAK SAIDLPRAQR ERFVALSQEI SEIGPEFVDY MTPAKPYLTF ESSKLKGMDP 250 260 270 280 290 300 VLVRQYTTWG QTKIPTIGGA AAAAIRSVQN EDVRKEIFMA TRTASRNTVY KLEELMRKRA 310 320 330 340 350 360 ELAKLSRYES YSHLALGDKM AKSPASVSQF LEALSKDNNQ IVEGEVSELL KFKMSNSHGS 370 380 390 400 410 420 SPGLQPWDKD YYMSQILASV RSHSRNSDFL SAYFSLGTVM QGLSRLFTRL YGVRLAPHET 430 440 450 460 470 480 MPGETWNSDV RRLDVISETD GHVAVLYCDL FSRPGKSPNP AHFTLRCSRE ITTPELEEAS 490 500 510 520 530 540 SLSQNGLFKT NEEAANDGMA TSRASGVLKQ LPTIALICDF VTMSGKSSRP ALLSFNEVQT 550 560 570 580 590 600 LFHEMGHAIH SILGRTSLQN VSGTRCATDF AELPSVLMEH FAADPSVLSL FARHYETDQP 610 620 630 640 650 660 LPYEMVAEKL ALDKRFEGSD TENQIILSML DLAYHSDLPL SPSFSSTQIY HSLQQKHGAL 670 680 690 700 710 720 PVDPPGTCWQ GFFGHLFGYG STYYSYLFDR VLARRIWQVV FKDGEAGGSI QRDNGEKMKE 730 740 750 760 EVLKWGGGRD PWKCLAGVLD DGRVENGDEK AMAIVGSWGV KE

References

[1]

"Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea." Amselem J., Cuomo C.A., van Kan J.A.L., Viaud M., Benito E.P., Couloux A., Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S., Fournier E., Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M., Pradier J.-M., Quevillon E. , Sharon A., Simon A., ten Have A., Tudzynski B., Tudzynski P., Wincker P., Andrew M., Anthouard V., Beever R.E., Beffa R., Benoit I., Bouzid O., Brault B., Chen Z., Choquer M., Collemare J., Cotton P., Danchin E.G., Da Silva C., Gautier A., Giraud C., Giraud T., Gonzalez C., Grossetete S., Gueldener U., Henrissat B., Howlett B.J., Kodira C., Kretschmer M., Lappartient A., Leroch M., Levis C., Mauceli E., Neuveglise C., Oeser B., Pearson M., Poulain J., Poussereau N., Quesneville H., Rascle C., Schumacher J., Segurens B., Sexton A., Silva E., Sirven C., Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O., Zeng Q., Rollins J.A., Lebrun M.-H., Dickman M. PLoS Genet. 7:E1002230-E1002230(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B05.10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476940 Genomic DNA. Translation: EDN33764.1. Different initiation.
RefSeq	XP_001549090.1. XM_001549040.1.
3D structure databases
ProteinModelPortal	A6SHZ5.
ModBase	Search...
Protein-protein interaction databases
STRING	332648.A6SHZ5.
Protein family/group databases
MEROPS	M03.006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5429605.
KEGG	bfu:BC1G_12067.
Phylogenomic databases
eggNOG	COG0339.
KO	K01410.
OrthoDB	EOG4GJ2XS.
Family and domain databases
Gene3D	1.10.1370.10. 2 hits. 3.40.390.10. 2 hits.
InterPro	IPR024079. MetalloPept_cat_dom. IPR024077. Neurolysin/TOP_dom2. IPR001567. Pept_M3A_M3B. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP_BOTFB

Accession

Primary (citable) accession number: A6SHZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	April 3, 2013
This is version 35 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program