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Protein

Mitochondrial intermediate peptidase

Gene

oct1

Organism
Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane (By similarity).By similarity

Catalytic activityi

Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi543Zinc; catalyticPROSITE-ProRule annotation1
Active sitei544PROSITE-ProRule annotation1
Metal bindingi547Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi550Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM03.006

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial intermediate peptidase (EC:3.4.24.59)
Short name:
MIP
Alternative name(s):
Octapeptidyl aminopeptidase
Gene namesi
Name:oct1
ORF Names:BC1G_12067
OrganismiBotryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
Taxonomic identifieri332648 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeBotrytis

Organism-specific databases

EuPathDBiFungiDB:BC1G_12067

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000338575‹1 – 762Mitochondrial intermediate peptidaseAdd BLAST›762

Proteomic databases

PRIDEiA6SHZ5

Structurei

3D structure databases

ProteinModelPortaliA6SHZ5
SMRiA6SHZ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi260 – 264Poly-Ala5

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Phylogenomic databases

KOiK01410

Family and domain databases

CDDicd06457 M3A_MIP, 1 hit
Gene3Di1.10.1370.10, 2 hits
InterProiView protein in InterPro
IPR033851 M3A_MIP
IPR024077 Neurolysin/TOP_dom2
IPR001567 Pept_M3A_M3B
PANTHERiPTHR11804:SF5 PTHR11804:SF5, 1 hit
PfamiView protein in Pfam
PF01432 Peptidase_M3, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Fragment.

A6SHZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
RRLATASTQY RESRPVPVDN SAPGAKRDDR TLRQIFDSPN FWAEFSQSSK
60 70 80 90 100
QSYNRPAVGL FQNRYLVNPQ GFEVFANTSL RKAQRIVDKV LSASTVEEYR
110 120 130 140 150
HVARELDRLS DLLCRVIDLS DFVRATHPNA AIQAAASRAY AKMFEYMNIL
160 170 180 190 200
NTTTGLDKQL EIAMATPEIV AGWTEEEVVV ADILRKDFAK SAIDLPRAQR
210 220 230 240 250
ERFVALSQEI SEIGPEFVDY MTPAKPYLTF ESSKLKGMDP VLVRQYTTWG
260 270 280 290 300
QTKIPTIGGA AAAAIRSVQN EDVRKEIFMA TRTASRNTVY KLEELMRKRA
310 320 330 340 350
ELAKLSRYES YSHLALGDKM AKSPASVSQF LEALSKDNNQ IVEGEVSELL
360 370 380 390 400
KFKMSNSHGS SPGLQPWDKD YYMSQILASV RSHSRNSDFL SAYFSLGTVM
410 420 430 440 450
QGLSRLFTRL YGVRLAPHET MPGETWNSDV RRLDVISETD GHVAVLYCDL
460 470 480 490 500
FSRPGKSPNP AHFTLRCSRE ITTPELEEAS SLSQNGLFKT NEEAANDGMA
510 520 530 540 550
TSRASGVLKQ LPTIALICDF VTMSGKSSRP ALLSFNEVQT LFHEMGHAIH
560 570 580 590 600
SILGRTSLQN VSGTRCATDF AELPSVLMEH FAADPSVLSL FARHYETDQP
610 620 630 640 650
LPYEMVAEKL ALDKRFEGSD TENQIILSML DLAYHSDLPL SPSFSSTQIY
660 670 680 690 700
HSLQQKHGAL PVDPPGTCWQ GFFGHLFGYG STYYSYLFDR VLARRIWQVV
710 720 730 740 750
FKDGEAGGSI QRDNGEKMKE EVLKWGGGRD PWKCLAGVLD DGRVENGDEK
760
AMAIVGSWGV KE
Length:762
Mass (Da):85,181
Last modified:June 10, 2008 - v2
Checksum:i383090D54BF577B9
GO

Sequence cautioni

The sequence EDN33764 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476940 Genomic DNA Translation: EDN33764.1 Different initiation.
RefSeqiXP_001549090.1, XM_001549040.1

Genome annotation databases

GeneIDi5429605
KEGGibfu:BC1G_12067

Similar proteinsi

Entry informationi

Entry nameiPMIP_BOTFB
AccessioniPrimary (citable) accession number: A6SHZ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: May 23, 2018
This is version 57 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

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