A6SDE9 (AMPP3_BOTFB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable Xaa-Pro aminopeptidase pepP
EC=3.4.11.9

Alternative name(s):
Aminoacylproline aminopeptidase
Prolidase

Gene names

Name:	pepP
ORF Names:	BC1G_10932

Organism

Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)

Taxonomic identifier

332648 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Leotiomycetes › Helotiales › Sclerotiniaceae › Botryotinia › Botryotinia fuckeliana

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides By similarity.
Catalytic activity	Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Sequence similarities	Belongs to the peptidase M24B family.

Ontologies

Keywords
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological_process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 458	458	Probable Xaa-Pro aminopeptidase pepP		PRO_0000411868
Sites
	Metal binding	254	1	Manganese 2 By similarity
	Metal binding	265	1	Manganese 1 By similarity
	Metal binding	265	1	Manganese 2 By similarity
	Metal binding	388	1	Manganese 1 By similarity
	Metal binding	428	1	Manganese 1 By similarity
	Metal binding	428	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6SDE9 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 5F83467C5B6C1571	FASTA	458	51,624
	10 20 30 40 50 60 MASSTDTILK GKYPAKEHVR KVVEYIKSKE PEAEGVLYLE AQKTIMIEDN DEAAPFRQRR 70 80 90 100 110 120 FFYYLTGCDL PDAYFTYDIA TDKSTLFIPP IDPESVIWTG LPLSPKEALA LYDVDEVLTT 130 140 150 160 170 180 DTINAQLALP NQTKVWAIAP QISTHITFLE FPQKDFTLLK EAIEEARVRK SEYEVALMRK 190 200 210 220 230 240 ANEISKVGHT AVLKAVKHAK NERELEALFI KESIANGARE QAYHSIVASG TAAATLHYMK 250 260 270 280 290 300 NSEELDGKLN LLLDAGGEYK CYASDITRTF PINGRFTPES RSIYDIVLSM QSQCISMLKA 310 320 330 340 350 360 GVSWDEVHLL AHKVAIEGLL SLGILKGDKE EILKARTSVA FFPHGLGHYL GMDTHDTGGH 370 380 390 400 410 420 PNYEDKDRLF RYLRVRGNLP EGSVVTVEPG IYFCRFIIEP YLKDPAHAQY INSDILEKYW 430 440 450 EVGGVRIEDN VLITKDGYDN LTTVVKDVEE MEKIINES

References

[1]

"Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea." Amselem J., Cuomo C.A., van Kan J.A.L., Viaud M., Benito E.P., Couloux A., Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S., Fournier E., Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M., Pradier J.-M., Quevillon E. , Sharon A., Simon A., ten Have A., Tudzynski B., Tudzynski P., Wincker P., Andrew M., Anthouard V., Beever R.E., Beffa R., Benoit I., Bouzid O., Brault B., Chen Z., Choquer M., Collemare J., Cotton P., Danchin E.G., Da Silva C., Gautier A., Giraud C., Giraud T., Gonzalez C., Grossetete S., Gueldener U., Henrissat B., Howlett B.J., Kodira C., Kretschmer M., Lappartient A., Leroch M., Levis C., Mauceli E., Neuveglise C., Oeser B., Pearson M., Poulain J., Poussereau N., Quesneville H., Rascle C., Schumacher J., Segurens B., Sexton A., Silva E., Sirven C., Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O., Zeng Q., Rollins J.A., Lebrun M.-H., Dickman M. PLoS Genet. 7:E1002230-E1002230(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B05.10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476916 Genomic DNA. Translation: EDN31731.1.
RefSeq	XP_001550759.1. XM_001550709.1.
3D structure databases
ProteinModelPortal	A6SDE9.
ModBase	Search...
Protein-protein interaction databases
STRING	332648.A6SDE9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5431260.
KEGG	bfu:BC1G_10932.
Phylogenomic databases
eggNOG	COG0006.
KO	K14213.
OMA	ITIEPGC.
OrthoDB	EOG4J40RD.
Family and domain databases
Gene3D	3.90.230.10. 1 hit.
InterPro	IPR007865. Aminopep_P_N. IPR000994. Pept_M24_structural-domain. [Graphical view]
Pfam	PF05195. AMP_N. 1 hit. PF00557. Peptidase_M24. 1 hit. [Graphical view]
SMART	SM01011. AMP_N. 1 hit. [Graphical view]
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
PROSITE	PS00491. PROLINE_PEPTIDASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPP3_BOTFB

Accession

Primary (citable) accession number: A6SDE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	August 21, 2007
Last modified:	April 3, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program