Reviewed, UniProtKB/Swiss-Prot A6SAG8 (LKHA4_BOTFB)

Names and origin

Protein names

Recommended name:
    Leukotriene A-4 hydrolase
    EC=3.3.2.6
Alternative name(s):
    Leukotriene A(4) hydrolase
      Short name=LTA-4 hydrolase

Gene names

ORF Names:

BC1G_09514

Organism

Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea) [Complete proteome]

Taxonomic identifier

332648 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Leotiomycetes › Helotiales › Sclerotiniaceae › Botryotinia

Protein attributes

Sequence length	616 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes an epoxide moiety of LTA4 to form LTB4. The enzyme also has some peptidase activity By similarity.
Catalytic activity	(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O = (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Lipid metabolism; leukotriene B4 biosynthesis.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase M1 family.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	leukotriene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	leukotriene-A4 hydrolase activity Inferred from electronic annotation. Source: EC metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 616	616	Leukotriene A-4 hydrolase		PRO_0000324923
Sites
	Active site	307	1	By similarity
	Active site	394	1	Proton donor By similarity
	Metal binding	306	1	Zinc; catalytic By similarity
	Metal binding	310	1	Zinc; catalytic By similarity
	Metal binding	329	1	Zinc; catalytic By similarity
	Site	210	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6SAG8-1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 08A0FB6C96FAC325	FASTA	616	69,504
	10 20 30 40 50 60 MATVSTINMP RDPNTLSNYN NWRTKHTIAD LAIDFTKQRV HGTVTLQLES ITDKESEEII 70 80 90 100 110 120 LDTSFVDVQS VAVDGNKTGE WTLEKRIEPF GTPLSVKIPG GAAKGTVIAL AITLSTTDKC 130 140 150 160 170 180 TALQWLTPAQ TSNKKFPYMF SQCQAIHNRS IFPCQDTPDV KSTYDFRIRS PLPVLASGLP 190 200 210 220 230 240 RGAGSFVHGE NGESGTLLYS FYQEIPMPSY LFALASGDIA TASIGPRSLV STGPEELIGA 250 260 270 280 290 300 KWELERDTEK FIETIEKIVY PYEWTQYNVL VLPPSFPYGG MENPVFTFAT PTIISGDREN 310 320 330 340 350 360 VDVVAHELAH SWSGNLVSNA SWEHFWLNEG WTVYLERRII AAVHGEAYRD FSSIIGWKAL 370 380 390 400 410 420 EDSVKLYGED HEFTKLIVDL KGKDPDDAFS SVPYEKGFHF LYYLERLVGK PSWDKFIPHY 430 440 450 460 470 480 FTTWKKKSLD SYDFKATLLD FFASDSAASK ALESVDWDSW FYKPGLPSKP EFDTSLVDKC 490 500 510 520 530 540 YALAKKWESK DYTPSPSDIE GWAANQVVVF LQQVQLFTTP LTPVQSQAMG KAYNLVNTKN 550 560 570 580 590 600 VELSSRYFGV GLAAKDETVY QPTAELLGKV GRMKFVRTLY RKLVVVDRKL AVETFEKNKD 610 FYHPICRDQV EKDLKE

References

[1]

"Annotation of the Botryotinia fuckeliana B05.10 genome." Birren B.W., Galagan J.E., Lander E.S., Devon K., Nusbaum C., Cuomo C., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K., Pushparaj V. , DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Alvarado L., Kodira C.D., Zeng Q., Yandava C., O'Leary S., Amedeo P., Briggs S., Baker S., Goff S., Hohn T., Hutchison D., Lam S., Martin C., Miguel T., Rose M., Turgeon B.G., Wu G., Yoder O., Zheng L. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476904 Genomic DNA. Translation: EDN30453.1. Different initiation.
RefSeq	XP_001551808.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5432324.
KEGG	bfu:BC1G_09514.
Family and domain databases
InterPro	IPR012777. Leuk_A4_hydro_aminopept. IPR006025. Pept_M_Zn_BS. IPR001930. Peptidase_M1. IPR015211. Peptidase_M1_C. IPR014782. Peptidase_M1_N. [Graphical view]
PANTHER	PTHR11533. Peptidase_M1. 1 hit.
Pfam	PF09127. Leuk-A4-hydro_C. 1 hit. PF01433. Peptidase_M1. 1 hit. [Graphical view]
PRINTS	PR00756. ALADIPTASE.
TIGRFAMs	TIGR02411. leuko_A4_hydro. 1 hit.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LKHA4_BOTFB

Accession

Primary (citable) accession number: A6SAG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	October 13, 2009
This is version 18 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)