Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A6S8E7

- TRMB_BOTFB

UniProt

A6S8E7 - TRMB_BOTFB

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trm8

Organism
Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei100 – 1001S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei178 – 1781S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei181 – 1811UniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    Transfer RNA methyltransferase 8UniRule annotation
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trm8
    ORF Names:BC1G_09638
    OrganismiBotryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
    Taxonomic identifieri332648 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeBotrytis

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 282282tRNA (guanine-N(7)-)-methyltransferasePRO_0000370590Add
    BLAST

    Interactioni

    Subunit structurei

    Forms a complex with trm82.UniRule annotation

    Protein-protein interaction databases

    STRINGi332648.A6S8E7.

    Structurei

    3D structure databases

    ProteinModelPortaliA6S8E7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni123 – 1242S-adenosyl-L-methionine bindingUniRule annotation
    Regioni158 – 1592S-adenosyl-L-methionine bindingUniRule annotation
    Regioni256 – 2583S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    KOiK03439.
    OMAiRAHSNPI.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6S8E7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGPPNKKQK REDYRTAREN GEESKELPKK KFYRQRAHAN PFSDHRLAYP    50
    ASPAQMDWAS HYPAYAESAA ASEGTEEPST GSDSSSLKKL KQDVEVADIG 100
    CGFGGLTVAL APKLPNSLIL GMEIRAQVTE YVQERIKALR VQEKESGLFQ 150
    NASCIRANTM KFMPNFFKKH QLSKIFLCFP DPHFKARKHK ARIVSTTLAA 200
    EYAYVVRPGG IIYTITDVED LHNWMVTHFN AHPTFERVAE EEQEADECVN 250
    IMRTETEEGK KVTRNNGPKF VALFKRLEDP PW 282
    Length:282
    Mass (Da):31,978
    Last modified:August 21, 2007 - v1
    Checksum:iA9F582329AB3CF2B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH476897 Genomic DNA. Translation: EDN29465.1.
    RefSeqiXP_001552408.1. XM_001552358.1.

    Genome annotation databases

    GeneIDi5432926.
    KEGGibfu:BC1G_09638.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH476897 Genomic DNA. Translation: EDN29465.1 .
    RefSeqi XP_001552408.1. XM_001552358.1.

    3D structure databases

    ProteinModelPortali A6S8E7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 332648.A6S8E7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5432926.
    KEGGi bfu:BC1G_09638.

    Phylogenomic databases

    eggNOGi COG0220.
    KOi K03439.
    OMAi RAHSNPI.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea."
      Amselem J., Cuomo C.A., van Kan J.A.L., Viaud M., Benito E.P., Couloux A., Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S., Fournier E., Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M., Pradier J.-M., Quevillon E.
      , Sharon A., Simon A., ten Have A., Tudzynski B., Tudzynski P., Wincker P., Andrew M., Anthouard V., Beever R.E., Beffa R., Benoit I., Bouzid O., Brault B., Chen Z., Choquer M., Collemare J., Cotton P., Danchin E.G., Da Silva C., Gautier A., Giraud C., Giraud T., Gonzalez C., Grossetete S., Gueldener U., Henrissat B., Howlett B.J., Kodira C., Kretschmer M., Lappartient A., Leroch M., Levis C., Mauceli E., Neuveglise C., Oeser B., Pearson M., Poulain J., Poussereau N., Quesneville H., Rascle C., Schumacher J., Segurens B., Sexton A., Silva E., Sirven C., Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O., Zeng Q., Rollins J.A., Lebrun M.-H., Dickman M.
      PLoS Genet. 7:E1002230-E1002230(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: B05.10.

    Entry informationi

    Entry nameiTRMB_BOTFB
    AccessioniPrimary (citable) accession number: A6S8E7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3