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A6S8E7 (TRMB_BOTFB) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trm8
ORF Names:BC1G_09638
OrganismBotryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
Taxonomic identifier332648 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeBotrytis

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with trm82 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 282282tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370590

Regions

Region123 – 1242S-adenosyl-L-methionine binding By similarity
Region158 – 1592S-adenosyl-L-methionine binding By similarity
Region256 – 2583S-adenosyl-L-methionine binding By similarity

Sites

Active site1811 By similarity
Binding site1001S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1781S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
A6S8E7 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: A9F582329AB3CF2B

FASTA28231,978
        10         20         30         40         50         60 
MAGPPNKKQK REDYRTAREN GEESKELPKK KFYRQRAHAN PFSDHRLAYP ASPAQMDWAS 

        70         80         90        100        110        120 
HYPAYAESAA ASEGTEEPST GSDSSSLKKL KQDVEVADIG CGFGGLTVAL APKLPNSLIL 

       130        140        150        160        170        180 
GMEIRAQVTE YVQERIKALR VQEKESGLFQ NASCIRANTM KFMPNFFKKH QLSKIFLCFP 

       190        200        210        220        230        240 
DPHFKARKHK ARIVSTTLAA EYAYVVRPGG IIYTITDVED LHNWMVTHFN AHPTFERVAE 

       250        260        270        280 
EEQEADECVN IMRTETEEGK KVTRNNGPKF VALFKRLEDP PW 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH476897 Genomic DNA. Translation: EDN29465.1.
RefSeqXP_001552408.1. XM_001552358.1.

3D structure databases

ProteinModelPortalA6S8E7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING332648.A6S8E7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5432926.
KEGGbfu:BC1G_09638.

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMARAHSNPI.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_BOTFB
AccessionPrimary (citable) accession number: A6S8E7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: August 21, 2007
Last modified: July 9, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways