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A6S8E7

- TRMB_BOTFB

UniProt

A6S8E7 - TRMB_BOTFB

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene
trm8, BC1G_09638
Organism
Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei178 – 1781S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei181 – 1811 By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trm8
ORF Names:BC1G_09638
OrganismiBotryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
Taxonomic identifieri332648 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeBotrytis

Subcellular locationi

Nucleus By similarity UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282tRNA (guanine-N(7)-)-methyltransferaseUniRule annotationPRO_0000370590Add
BLAST

Interactioni

Subunit structurei

Forms a complex with trm82 By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi332648.A6S8E7.

Structurei

3D structure databases

ProteinModelPortaliA6S8E7.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1242S-adenosyl-L-methionine binding By similarity
Regioni158 – 1592S-adenosyl-L-methionine binding By similarity
Regioni256 – 2583S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OMAiRAHSNPI.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6S8E7-1 [UniParc]FASTAAdd to Basket

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MAGPPNKKQK REDYRTAREN GEESKELPKK KFYRQRAHAN PFSDHRLAYP    50
ASPAQMDWAS HYPAYAESAA ASEGTEEPST GSDSSSLKKL KQDVEVADIG 100
CGFGGLTVAL APKLPNSLIL GMEIRAQVTE YVQERIKALR VQEKESGLFQ 150
NASCIRANTM KFMPNFFKKH QLSKIFLCFP DPHFKARKHK ARIVSTTLAA 200
EYAYVVRPGG IIYTITDVED LHNWMVTHFN AHPTFERVAE EEQEADECVN 250
IMRTETEEGK KVTRNNGPKF VALFKRLEDP PW 282
Length:282
Mass (Da):31,978
Last modified:August 21, 2007 - v1
Checksum:iA9F582329AB3CF2B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH476897 Genomic DNA. Translation: EDN29465.1.
RefSeqiXP_001552408.1. XM_001552358.1.

Genome annotation databases

GeneIDi5432926.
KEGGibfu:BC1G_09638.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH476897 Genomic DNA. Translation: EDN29465.1 .
RefSeqi XP_001552408.1. XM_001552358.1.

3D structure databases

ProteinModelPortali A6S8E7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 332648.A6S8E7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5432926.
KEGGi bfu:BC1G_09638.

Phylogenomic databases

eggNOGi COG0220.
KOi K03439.
OMAi RAHSNPI.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea."
    Amselem J., Cuomo C.A., van Kan J.A.L., Viaud M., Benito E.P., Couloux A., Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S., Fournier E., Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M., Pradier J.-M., Quevillon E.
    , Sharon A., Simon A., ten Have A., Tudzynski B., Tudzynski P., Wincker P., Andrew M., Anthouard V., Beever R.E., Beffa R., Benoit I., Bouzid O., Brault B., Chen Z., Choquer M., Collemare J., Cotton P., Danchin E.G., Da Silva C., Gautier A., Giraud C., Giraud T., Gonzalez C., Grossetete S., Gueldener U., Henrissat B., Howlett B.J., Kodira C., Kretschmer M., Lappartient A., Leroch M., Levis C., Mauceli E., Neuveglise C., Oeser B., Pearson M., Poulain J., Poussereau N., Quesneville H., Rascle C., Schumacher J., Segurens B., Sexton A., Silva E., Sirven C., Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O., Zeng Q., Rollins J.A., Lebrun M.-H., Dickman M.
    PLoS Genet. 7:E1002230-E1002230(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B05.10.

Entry informationi

Entry nameiTRMB_BOTFB
AccessioniPrimary (citable) accession number: A6S8E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: August 21, 2007
Last modified: July 9, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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