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A6RPN7

- BGALB_BOTFB

UniProt

A6RPN7 - BGALB_BOTFB

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Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871SubstrateBy similarity
Binding sitei132 – 1321SubstrateBy similarity
Binding sitei133 – 1331Substrate; via amide nitrogenBy similarity
Binding sitei134 – 1341SubstrateBy similarity
Binding sitei192 – 1921SubstrateBy similarity
Active sitei193 – 1931Proton donorSequence Analysis
Binding sitei262 – 2621SubstrateBy similarity
Active sitei305 – 3051NucleophileSequence Analysis
Binding sitei370 – 3701SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:BC1G_02410
OrganismiBotryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
Taxonomic identifieri332648 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeBotrytis

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 1007989Probable beta-galactosidase BPRO_0000395228Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi20 – 201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi23 – 231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi155 – 1551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi268 ↔ 321By similarity
Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi409 – 4091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi453 – 4531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi594 – 5941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi624 – 6241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi681 – 6811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi703 – 7031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi787 – 7871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi815 – 8151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi825 – 8251N-linked (GlcNAc...)Sequence Analysis
Glycosylationi878 – 8781N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi332648.A6RPN7.

Structurei

3D structure databases

ProteinModelPortaliA6RPN7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
OMAiYINANTS.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6RPN7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLQSLFASA LTLGSCVAQN STNGTWPIHN NGLTTQIEWD HYSMIVNGQR
60 70 80 90 100
FFLWSGELHY WRIPVPELWV DVLQKIKAAG FNTFAIYTHW YFHNPNPETL
110 120 130 140 150
DFENAAHDFT KIFDLAKELG MYVVFRPGPY VNAESNAGAF PLWLTTGAYG
160 170 180 190 200
SLRNNDTRYT EAWTPFWEKV ASIVAPYQLT NGGNVLTYQI ENELGSQWRG
210 220 230 240 250
TPSKKIPNLS SIQYMEALEA SARAQGITIP FQANDPNLNS DSWSKDFYDG
260 270 280 290 300
YGSVDIYGMD SYPACWTCNL TECDSTNGAY KPFNVIDYYD HFQEISPTQP
310 320 330 340 350
SFLPEFQGGS FNPWGGPEGG CPENSPADFA NLFYRNNVGQ RVTAMSLYML
360 370 380 390 400
YGGTNWGWLA APVVATSYDY SSPISENRMI NDKYAETKLF GQFLRVAKDL
410 420 430 440 450
TKTDRIGTNQ TASTNPNIVY SEIRNPDTNG AFYVTIHQES TVGTREEFYI
460 470 480 490 500
NANTSKGEFT IPQKAAPIVL NGFQSKIIVT DFSFGSHSLL YSTAEVLSHS
510 520 530 540 550
IVDEEDILAL WMPTGEAGEF VVTGAKSGKV SSCGGCSSVG FYPQGDDLLV
560 570 580 590 600
TISQSEGISI LTFDDGLRVL VMDRSYAYEF WVPVLTADPF SPANETVFVQ
610 620 630 640 650
GPSLVRSVAY SSDGTILELT GDNNGTSTQI EVFPPKAVSE VTWNGQSIKT
660 670 680 690 700
SKTDYGSLIG SLTAPALDSL TLPEISGWKA NDSLPERLST YDDSWWVDAN
710 720 730 740 750
HMNTSNPTKP ETLPVLYIDD YGYHVGNHLW RGRFEGSASG VYLSVTGGRA
760 770 780 790 800
FGYSAWLNGE FIGSYVGAAY PDTGELTLSF GNLASSNSTN ILLVLQDNSG
810 820 830 840 850
HDETSGALNP RGINNATLIS SSAKNFTSWK VTGTAGKPDT AIDPVRGILS
860 870 880 890 900
EGGLYAERLG WHLPGFDDSE WSSASPSNIS SSAGVTFYRT TVPLAIPTGL
910 920 930 940 950
DVAISFTLNA SPSNAALRAL LFVNGYQYGR FSPWIGNQIE FPVPPGILNY
960 970 980 990 1000
DGDNVIGLSV WRQEEGGESM GVDVGWKITE AFASSFEPIF DATYLQPGWT

EERLQYA
Length:1,007
Mass (Da):110,544
Last modified:August 21, 2007 - v1
Checksum:iB78768183EDEE67F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476848 Genomic DNA. Translation: EDN31260.1.
RefSeqiXP_001559246.1. XM_001559196.1.

Genome annotation databases

GeneIDi5439872.
KEGGibfu:BC1G_02410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476848 Genomic DNA. Translation: EDN31260.1 .
RefSeqi XP_001559246.1. XM_001559196.1.

3D structure databases

ProteinModelPortali A6RPN7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 332648.A6RPN7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5439872.
KEGGi bfu:BC1G_02410.

Phylogenomic databases

eggNOGi COG1874.
OMAi YINANTS.

Family and domain databases

Gene3Di 2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SUPFAMi SSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea."
    Amselem J., Cuomo C.A., van Kan J.A.L., Viaud M., Benito E.P., Couloux A., Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S., Fournier E., Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M., Pradier J.-M., Quevillon E.
    , Sharon A., Simon A., ten Have A., Tudzynski B., Tudzynski P., Wincker P., Andrew M., Anthouard V., Beever R.E., Beffa R., Benoit I., Bouzid O., Brault B., Chen Z., Choquer M., Collemare J., Cotton P., Danchin E.G., Da Silva C., Gautier A., Giraud C., Giraud T., Gonzalez C., Grossetete S., Gueldener U., Henrissat B., Howlett B.J., Kodira C., Kretschmer M., Lappartient A., Leroch M., Levis C., Mauceli E., Neuveglise C., Oeser B., Pearson M., Poulain J., Poussereau N., Quesneville H., Rascle C., Schumacher J., Segurens B., Sexton A., Silva E., Sirven C., Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O., Zeng Q., Rollins J.A., Lebrun M.-H., Dickman M.
    PLoS Genet. 7:E1002230-E1002230(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B05.10.

Entry informationi

Entry nameiBGALB_BOTFB
AccessioniPrimary (citable) accession number: A6RPN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: August 21, 2007
Last modified: October 29, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3