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Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87SubstrateBy similarity1
Binding sitei132SubstrateBy similarity1
Binding sitei133Substrate; via amide nitrogenBy similarity1
Binding sitei134SubstrateBy similarity1
Binding sitei192SubstrateBy similarity1
Active sitei193Proton donorSequence analysis1
Binding sitei262SubstrateBy similarity1
Active sitei305NucleophileSequence analysis1
Binding sitei370SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:BC1G_02410
OrganismiBotryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
Taxonomic identifieri332648 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeBotrytis

Organism-specific databases

EuPathDBiFungiDB:BC1G_02410.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000039522819 – 1007Probable beta-galactosidase BAdd BLAST989

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi20N-linked (GlcNAc...)Sequence analysis1
Glycosylationi23N-linked (GlcNAc...)Sequence analysis1
Glycosylationi155N-linked (GlcNAc...)Sequence analysis1
Glycosylationi208N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi268 ↔ 321By similarity
Glycosylationi269N-linked (GlcNAc...)Sequence analysis1
Glycosylationi409N-linked (GlcNAc...)Sequence analysis1
Glycosylationi453N-linked (GlcNAc...)Sequence analysis1
Glycosylationi594N-linked (GlcNAc...)Sequence analysis1
Glycosylationi624N-linked (GlcNAc...)Sequence analysis1
Glycosylationi681N-linked (GlcNAc...)Sequence analysis1
Glycosylationi703N-linked (GlcNAc...)Sequence analysis1
Glycosylationi787N-linked (GlcNAc...)Sequence analysis1
Glycosylationi815N-linked (GlcNAc...)Sequence analysis1
Glycosylationi825N-linked (GlcNAc...)Sequence analysis1
Glycosylationi878N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliA6RPN7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

OMAiYINANTS.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6RPN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLQSLFASA LTLGSCVAQN STNGTWPIHN NGLTTQIEWD HYSMIVNGQR
60 70 80 90 100
FFLWSGELHY WRIPVPELWV DVLQKIKAAG FNTFAIYTHW YFHNPNPETL
110 120 130 140 150
DFENAAHDFT KIFDLAKELG MYVVFRPGPY VNAESNAGAF PLWLTTGAYG
160 170 180 190 200
SLRNNDTRYT EAWTPFWEKV ASIVAPYQLT NGGNVLTYQI ENELGSQWRG
210 220 230 240 250
TPSKKIPNLS SIQYMEALEA SARAQGITIP FQANDPNLNS DSWSKDFYDG
260 270 280 290 300
YGSVDIYGMD SYPACWTCNL TECDSTNGAY KPFNVIDYYD HFQEISPTQP
310 320 330 340 350
SFLPEFQGGS FNPWGGPEGG CPENSPADFA NLFYRNNVGQ RVTAMSLYML
360 370 380 390 400
YGGTNWGWLA APVVATSYDY SSPISENRMI NDKYAETKLF GQFLRVAKDL
410 420 430 440 450
TKTDRIGTNQ TASTNPNIVY SEIRNPDTNG AFYVTIHQES TVGTREEFYI
460 470 480 490 500
NANTSKGEFT IPQKAAPIVL NGFQSKIIVT DFSFGSHSLL YSTAEVLSHS
510 520 530 540 550
IVDEEDILAL WMPTGEAGEF VVTGAKSGKV SSCGGCSSVG FYPQGDDLLV
560 570 580 590 600
TISQSEGISI LTFDDGLRVL VMDRSYAYEF WVPVLTADPF SPANETVFVQ
610 620 630 640 650
GPSLVRSVAY SSDGTILELT GDNNGTSTQI EVFPPKAVSE VTWNGQSIKT
660 670 680 690 700
SKTDYGSLIG SLTAPALDSL TLPEISGWKA NDSLPERLST YDDSWWVDAN
710 720 730 740 750
HMNTSNPTKP ETLPVLYIDD YGYHVGNHLW RGRFEGSASG VYLSVTGGRA
760 770 780 790 800
FGYSAWLNGE FIGSYVGAAY PDTGELTLSF GNLASSNSTN ILLVLQDNSG
810 820 830 840 850
HDETSGALNP RGINNATLIS SSAKNFTSWK VTGTAGKPDT AIDPVRGILS
860 870 880 890 900
EGGLYAERLG WHLPGFDDSE WSSASPSNIS SSAGVTFYRT TVPLAIPTGL
910 920 930 940 950
DVAISFTLNA SPSNAALRAL LFVNGYQYGR FSPWIGNQIE FPVPPGILNY
960 970 980 990 1000
DGDNVIGLSV WRQEEGGESM GVDVGWKITE AFASSFEPIF DATYLQPGWT

EERLQYA
Length:1,007
Mass (Da):110,544
Last modified:August 21, 2007 - v1
Checksum:iB78768183EDEE67F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476848 Genomic DNA. Translation: EDN31260.1.
RefSeqiXP_001559246.1. XM_001559196.1.

Genome annotation databases

GeneIDi5439872.
KEGGibfu:BC1G_02410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476848 Genomic DNA. Translation: EDN31260.1.
RefSeqiXP_001559246.1. XM_001559196.1.

3D structure databases

ProteinModelPortaliA6RPN7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi5439872.
KEGGibfu:BC1G_02410.

Organism-specific databases

EuPathDBiFungiDB:BC1G_02410.

Phylogenomic databases

OMAiYINANTS.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBGALB_BOTFB
AccessioniPrimary (citable) accession number: A6RPN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: August 21, 2007
Last modified: May 11, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.