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A6RPN7

- BGALB_BOTFB

UniProt

A6RPN7 - BGALB_BOTFB

Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei87 – 871SubstrateBy similarity
    Binding sitei132 – 1321SubstrateBy similarity
    Binding sitei133 – 1331Substrate; via amide nitrogenBy similarity
    Binding sitei134 – 1341SubstrateBy similarity
    Binding sitei192 – 1921SubstrateBy similarity
    Active sitei193 – 1931Proton donorSequence Analysis
    Binding sitei262 – 2621SubstrateBy similarity
    Active sitei305 – 3051NucleophileSequence Analysis
    Binding sitei370 – 3701SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable beta-galactosidase B (EC:3.2.1.23)
    Alternative name(s):
    Lactase B
    Gene namesi
    Name:lacB
    ORF Names:BC1G_02410
    OrganismiBotryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
    Taxonomic identifieri332648 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeBotrytis

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 1007989Probable beta-galactosidase BPRO_0000395228Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi20 – 201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi23 – 231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi155 – 1551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi268 ↔ 321By similarity
    Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi409 – 4091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi453 – 4531N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi594 – 5941N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi624 – 6241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi681 – 6811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi703 – 7031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi787 – 7871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi815 – 8151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi825 – 8251N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi878 – 8781N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi332648.A6RPN7.

    Structurei

    3D structure databases

    ProteinModelPortaliA6RPN7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 35 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1874.
    OMAiYINANTS.

    Family and domain databases

    Gene3Di2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR23421. PTHR23421. 1 hit.
    PfamiPF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view]
    PRINTSiPR00742. GLHYDRLASE35.
    SUPFAMiSSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A6RPN7-1 [UniParc]FASTAAdd to Basket

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    MRLQSLFASA LTLGSCVAQN STNGTWPIHN NGLTTQIEWD HYSMIVNGQR     50
    FFLWSGELHY WRIPVPELWV DVLQKIKAAG FNTFAIYTHW YFHNPNPETL 100
    DFENAAHDFT KIFDLAKELG MYVVFRPGPY VNAESNAGAF PLWLTTGAYG 150
    SLRNNDTRYT EAWTPFWEKV ASIVAPYQLT NGGNVLTYQI ENELGSQWRG 200
    TPSKKIPNLS SIQYMEALEA SARAQGITIP FQANDPNLNS DSWSKDFYDG 250
    YGSVDIYGMD SYPACWTCNL TECDSTNGAY KPFNVIDYYD HFQEISPTQP 300
    SFLPEFQGGS FNPWGGPEGG CPENSPADFA NLFYRNNVGQ RVTAMSLYML 350
    YGGTNWGWLA APVVATSYDY SSPISENRMI NDKYAETKLF GQFLRVAKDL 400
    TKTDRIGTNQ TASTNPNIVY SEIRNPDTNG AFYVTIHQES TVGTREEFYI 450
    NANTSKGEFT IPQKAAPIVL NGFQSKIIVT DFSFGSHSLL YSTAEVLSHS 500
    IVDEEDILAL WMPTGEAGEF VVTGAKSGKV SSCGGCSSVG FYPQGDDLLV 550
    TISQSEGISI LTFDDGLRVL VMDRSYAYEF WVPVLTADPF SPANETVFVQ 600
    GPSLVRSVAY SSDGTILELT GDNNGTSTQI EVFPPKAVSE VTWNGQSIKT 650
    SKTDYGSLIG SLTAPALDSL TLPEISGWKA NDSLPERLST YDDSWWVDAN 700
    HMNTSNPTKP ETLPVLYIDD YGYHVGNHLW RGRFEGSASG VYLSVTGGRA 750
    FGYSAWLNGE FIGSYVGAAY PDTGELTLSF GNLASSNSTN ILLVLQDNSG 800
    HDETSGALNP RGINNATLIS SSAKNFTSWK VTGTAGKPDT AIDPVRGILS 850
    EGGLYAERLG WHLPGFDDSE WSSASPSNIS SSAGVTFYRT TVPLAIPTGL 900
    DVAISFTLNA SPSNAALRAL LFVNGYQYGR FSPWIGNQIE FPVPPGILNY 950
    DGDNVIGLSV WRQEEGGESM GVDVGWKITE AFASSFEPIF DATYLQPGWT 1000
    EERLQYA 1007
    Length:1,007
    Mass (Da):110,544
    Last modified:August 21, 2007 - v1
    Checksum:iB78768183EDEE67F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH476848 Genomic DNA. Translation: EDN31260.1.
    RefSeqiXP_001559246.1. XM_001559196.1.

    Genome annotation databases

    GeneIDi5439872.
    KEGGibfu:BC1G_02410.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH476848 Genomic DNA. Translation: EDN31260.1 .
    RefSeqi XP_001559246.1. XM_001559196.1.

    3D structure databases

    ProteinModelPortali A6RPN7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 332648.A6RPN7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5439872.
    KEGGi bfu:BC1G_02410.

    Phylogenomic databases

    eggNOGi COG1874.
    OMAi YINANTS.

    Family and domain databases

    Gene3Di 2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR23421. PTHR23421. 1 hit.
    Pfami PF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view ]
    PRINTSi PR00742. GLHYDRLASE35.
    SUPFAMi SSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea."
      Amselem J., Cuomo C.A., van Kan J.A.L., Viaud M., Benito E.P., Couloux A., Coutinho P.M., de Vries R.P., Dyer P.S., Fillinger S., Fournier E., Gout L., Hahn M., Kohn L., Lapalu N., Plummer K.M., Pradier J.-M., Quevillon E.
      , Sharon A., Simon A., ten Have A., Tudzynski B., Tudzynski P., Wincker P., Andrew M., Anthouard V., Beever R.E., Beffa R., Benoit I., Bouzid O., Brault B., Chen Z., Choquer M., Collemare J., Cotton P., Danchin E.G., Da Silva C., Gautier A., Giraud C., Giraud T., Gonzalez C., Grossetete S., Gueldener U., Henrissat B., Howlett B.J., Kodira C., Kretschmer M., Lappartient A., Leroch M., Levis C., Mauceli E., Neuveglise C., Oeser B., Pearson M., Poulain J., Poussereau N., Quesneville H., Rascle C., Schumacher J., Segurens B., Sexton A., Silva E., Sirven C., Soanes D.M., Talbot N.J., Templeton M., Yandava C., Yarden O., Zeng Q., Rollins J.A., Lebrun M.-H., Dickman M.
      PLoS Genet. 7:E1002230-E1002230(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: B05.10.

    Entry informationi

    Entry nameiBGALB_BOTFB
    AccessioniPrimary (citable) accession number: A6RPN7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 13, 2010
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3