Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A6RPN7 (BGALB_BOTFB) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable beta-galactosidase B

EC=3.2.1.23
Alternative name(s):
Lactase B
Gene names
Name:lacB
ORF Names:BC1G_02410
OrganismBotryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
Taxonomic identifier332648 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesHelotialesSclerotiniaceaeBotrytis

Protein attributes

Sequence length1007 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans By similarity.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Polysaccharide degradation
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological_processpolysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 1007989Probable beta-galactosidase B
PRO_0000395228

Sites

Active site1931Proton donor Potential
Active site3051Nucleophile Potential
Binding site871Substrate By similarity
Binding site1321Substrate By similarity
Binding site1331Substrate; via amide nitrogen By similarity
Binding site1341Substrate By similarity
Binding site1921Substrate By similarity
Binding site2621Substrate By similarity
Binding site3701Substrate By similarity

Amino acid modifications

Glycosylation201N-linked (GlcNAc...) Potential
Glycosylation231N-linked (GlcNAc...) Potential
Glycosylation1551N-linked (GlcNAc...) Potential
Glycosylation2081N-linked (GlcNAc...) Potential
Glycosylation2691N-linked (GlcNAc...) Potential
Glycosylation4091N-linked (GlcNAc...) Potential
Glycosylation4531N-linked (GlcNAc...) Potential
Glycosylation5941N-linked (GlcNAc...) Potential
Glycosylation6241N-linked (GlcNAc...) Potential
Glycosylation6811N-linked (GlcNAc...) Potential
Glycosylation7031N-linked (GlcNAc...) Potential
Glycosylation7871N-linked (GlcNAc...) Potential
Glycosylation8151N-linked (GlcNAc...) Potential
Glycosylation8251N-linked (GlcNAc...) Potential
Glycosylation8781N-linked (GlcNAc...) Potential
Disulfide bond268 ↔ 321 By similarity

Sequences

Sequence LengthMass (Da)Tools
A6RPN7 [UniParc].

Last modified August 21, 2007. Version 1.
Checksum: B78768183EDEE67F

FASTA1,007110,544
        10         20         30         40         50         60 
MRLQSLFASA LTLGSCVAQN STNGTWPIHN NGLTTQIEWD HYSMIVNGQR FFLWSGELHY 

        70         80         90        100        110        120 
WRIPVPELWV DVLQKIKAAG FNTFAIYTHW YFHNPNPETL DFENAAHDFT KIFDLAKELG 

       130        140        150        160        170        180 
MYVVFRPGPY VNAESNAGAF PLWLTTGAYG SLRNNDTRYT EAWTPFWEKV ASIVAPYQLT 

       190        200        210        220        230        240 
NGGNVLTYQI ENELGSQWRG TPSKKIPNLS SIQYMEALEA SARAQGITIP FQANDPNLNS 

       250        260        270        280        290        300 
DSWSKDFYDG YGSVDIYGMD SYPACWTCNL TECDSTNGAY KPFNVIDYYD HFQEISPTQP 

       310        320        330        340        350        360 
SFLPEFQGGS FNPWGGPEGG CPENSPADFA NLFYRNNVGQ RVTAMSLYML YGGTNWGWLA 

       370        380        390        400        410        420 
APVVATSYDY SSPISENRMI NDKYAETKLF GQFLRVAKDL TKTDRIGTNQ TASTNPNIVY 

       430        440        450        460        470        480 
SEIRNPDTNG AFYVTIHQES TVGTREEFYI NANTSKGEFT IPQKAAPIVL NGFQSKIIVT 

       490        500        510        520        530        540 
DFSFGSHSLL YSTAEVLSHS IVDEEDILAL WMPTGEAGEF VVTGAKSGKV SSCGGCSSVG 

       550        560        570        580        590        600 
FYPQGDDLLV TISQSEGISI LTFDDGLRVL VMDRSYAYEF WVPVLTADPF SPANETVFVQ 

       610        620        630        640        650        660 
GPSLVRSVAY SSDGTILELT GDNNGTSTQI EVFPPKAVSE VTWNGQSIKT SKTDYGSLIG 

       670        680        690        700        710        720 
SLTAPALDSL TLPEISGWKA NDSLPERLST YDDSWWVDAN HMNTSNPTKP ETLPVLYIDD 

       730        740        750        760        770        780 
YGYHVGNHLW RGRFEGSASG VYLSVTGGRA FGYSAWLNGE FIGSYVGAAY PDTGELTLSF 

       790        800        810        820        830        840 
GNLASSNSTN ILLVLQDNSG HDETSGALNP RGINNATLIS SSAKNFTSWK VTGTAGKPDT 

       850        860        870        880        890        900 
AIDPVRGILS EGGLYAERLG WHLPGFDDSE WSSASPSNIS SSAGVTFYRT TVPLAIPTGL 

       910        920        930        940        950        960 
DVAISFTLNA SPSNAALRAL LFVNGYQYGR FSPWIGNQIE FPVPPGILNY DGDNVIGLSV 

       970        980        990       1000 
WRQEEGGESM GVDVGWKITE AFASSFEPIF DATYLQPGWT EERLQYA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH476848 Genomic DNA. Translation: EDN31260.1.
RefSeqXP_001559246.1. XM_001559196.1.

3D structure databases

ProteinModelPortalA6RPN7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING332648.A6RPN7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5439872.
KEGGbfu:BC1G_02410.

Phylogenomic databases

eggNOGCOG1874.
OMAYINANTS.

Family and domain databases

Gene3D2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGALB_BOTFB
AccessionPrimary (citable) accession number: A6RPN7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: August 21, 2007
Last modified: July 9, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries