Reviewed, UniProtKB/Swiss-Prot A6REI4 (ATG15_AJECN)

Names and origin

Protein names

Recommended name:
    Putative lipase ATG15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15

Gene names

Name:	ATG15
ORF Names:	HCAG_08049

Organism

Ajellomyces capsulata (strain NAm1 / WU24) (Darling's disease fungus) (Histoplasma capsulatum)

Taxonomic identifier

339724 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › Ajellomycetaceae › Ajellomyces

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.
Sequence caution	The sequence EDN04383.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	Putative lipase ATG15		PRO_0000317957
Regions
	Transmembrane	1 – 21	21	Signal-anchor for type II membrane protein
	Topological domain	22 – 585	564	Lumenal By similarity
	Compositional bias	439 – 575	137	Thr-rich
Sites
	Active site	291	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	171	1	N-linked (GlcNAc...) Potential
	Glycosylation	193	1	N-linked (GlcNAc...) Potential
	Glycosylation	275	1	N-linked (GlcNAc...) Potential
	Glycosylation	340	1	N-linked (GlcNAc...) Potential
	Glycosylation	437	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A6REI4-1 [UniParc]. Last modified February 5, 2008. Version 2. Checksum: 5EF38668984D7B6B	FASTA	585	63,488
	10 20 30 40 50 60 MMGLDLLVSL LALSVSPCIA ATRSPLQIPV LDTQPIETGR EFTLRHMLHH GTYRDPLLHK 70 80 90 100 110 120 RLDIRPDTLL WAASENGEKR ESVPRFRVSS RPINIHRLSD RHVSVVEDYL SIARMTSSAV 130 140 150 160 170 180 TLSSDYWTLD EVDAPDVTDK ETVLSLAKMT SNAYIMIPGS GEWFDVVPPF NHSDSFGWDS 190 200 210 220 230 240 DGLRGHIYSD NTNSTIVVVL KGTSAAIFDG GGTTTNDKVN DNLFFSCCCA QGGHYFWRQV 250 260 270 280 290 300 CDCYSSTYTC NTACVRKALR QENRYYRAAL NLYSNITAMY PQSNIWVTGH SLGGAVSSLL 310 320 330 340 350 360 GMTYGLPVVT FEAVPEALPA SRLGLPPPPG TDPSSPQARN YTGAYHFGHT ADPIYMGTCN 370 380 390 400 410 420 GATSVCTLGG YAMESSCHTG QLCTYDTVED FGWRVGIGTH RIREVITDVI ERYDDVPTCA 430 440 450 460 470 480 PFTECVDCNN WKFFESNETT PTPTTTTTST STRTRTSTCK TPGWWGCLDP TTTPTTTATT 490 500 510 520 530 540 STTSTSTCKT PGWFGCKDPT TTSTVPTASP APTITPTSPP TTTASTTSTC ESPGWFGCND 550 560 570 580 PTSTTTFPVP STTSTSTPCS TPGWFWGCRD QTTTTSASPP ITSPP

References

[1]

"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed: 19717792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476664 Genomic DNA. Translation: EDN04383.1. Sequence problems.
RefSeq	XP_001536940.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5443371.
Family and domain databases
InterPro	IPR008262. Lipase_Ser_AS. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_AJECN

Accession

Primary (citable) accession number: A6REI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 20 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)