A6R400 (A6R400_AJECN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase [NADP] PIRNR PIRNR000108
EC=1.1.1.42 PIRNR PIRNR000108

Gene names

ORF Names:

HCAG_04358 EMBL EDN07848.1

Organism

Ajellomyces capsulata (strain NAm1 / WU24) (Darling's disease fungus) (Histoplasma capsulatum) [Complete proteome] EMBL EDN07848.1

Taxonomic identifier

339724 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Onygenales › Ajellomycetaceae › Ajellomyces › Ajellomyces capsulatus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. PIRNR PIRNR000108
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. PIRNR PIRNR000108 RuleBase RU004443

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle PIRNR PIRNR000108
Ligand	Magnesium PIRNR PIRNR000108 Manganese PIRNR PIRNR000108 Metal-binding PIRNR PIRNR000108 NADP PIRNR PIRNR000108 PIRSR PIRSR000108-4
Molecular function	Oxidoreductase PIRNR PIRNR000108
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isocitrate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	64 – 66	3	NADP By similarity PIRSR PIRSR000108-4
	Nucleotide binding	261 – 266	6	NADP By similarity PIRSR PIRSR000108-4
	Region	83 – 89	7	Substrate binding By similarity PIRSR PIRSR000108-2
Sites
	Binding site	66	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	71	1	NADP By similarity PIRSR PIRSR000108-4
	Binding site	98	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	121	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	247	1	NADP By similarity PIRSR PIRSR000108-4
	Binding site	279	1	NADP; via amide nitrogen and carbonyl oxygen By similarity PIRSR PIRSR000108-4
	Site	128	1	Critical for catalysis By similarity PIRSR PIRSR000108-1
	Site	199	1	Critical for catalysis By similarity PIRSR PIRSR000108-1

Sequences

	Sequence		Length	Mass (Da)
	A6R400 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: D25A8C9FC6A06D0D	FASTA	363	41,159
	10 20 30 40 50 60 MTGSRRKIKV KNPVVELDGD EMTRIIWQDI KDKGLEYRDQ TNDQVTIDSA EAIKKYGVGV 70 80 90 100 110 120 KCATITPDEA RVEEFKLKQM WLSPNGTIRN ILGGTVFREP IVIPRIPRLV PGWKKPIIIG 130 140 150 160 170 180 RHAFGDQYRA KDLVVPGPGT LELVYTPKGG QPEHIKVFDF EGGGVAMSQY NTDDSIAGFA 190 200 210 220 230 240 HASFKLALLK EMPLYMSTKN TILKKYDGRF KDIFQEIFEA NYKKDFDAKG IWYEHRLIDD 250 260 270 280 290 300 MVAQMIKSDG GFVIAMKTAH GTVTRHYREY QKGNETSTNP IASIFAWTRG LIRRGQLDNT 310 320 330 340 350 360 PDVVVWAEQL ERACIDVVDE DGVMTKDLAL ACGRKDRAAW VTTREYLEAV ERRLQKNLAN AKL

References

[1]

"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NAm1 / WU24.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476658 Genomic DNA. Translation: EDN07848.1.
RefSeq	XP_001540518.1. XM_001540468.1.
3D structure databases
ProteinModelPortal	A6R400.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5447074.
KEGG	aje:HCAG_04358.
Phylogenomic databases
KO	K00031.
Family and domain databases
Gene3D	3.40.718.10. 2 hits.
InterPro	IPR004790. Isocitrate_DH_NADP. IPR024084. IsoPropMal-DH-like_dom. [Graphical view]
PANTHER	PTHR11822. PTHR11822. 1 hit.
Pfam	PF00180. Iso_dh. 2 hits. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A6R400_AJECN

Accession

Primary (citable) accession number: A6R400

Entry history

Integrated into UniProtKB/TrEMBL:	August 21, 2007
Last sequence update:	August 21, 2007
Last modified:	March 6, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)