Reviewed, UniProtKB/Swiss-Prot A6R2K7 (NCB5R_AJECN)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 1
    EC=1.6.2.2
Alternative name(s):
    Microsomal cytochrome b reductase

Gene names

Name:	CBR1
ORF Names:	HCAG_03865

Organism

Ajellomyces capsulata (strain NAm1 / WU24) (Darling's disease fungus) (Histoplasma capsulatum)

Taxonomic identifier

339724 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › Ajellomycetaceae › Ajellomyces

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	NADH-cytochrome b5 reductase 1		PRO_0000330142
Regions
	Transmembrane	32 – 52	21	Potential
	Domain	61 – 166	106	FAD-binding FR-type
	Nucleotide binding	146 – 161	16	FAD By similarity
	Nucleotide binding	172 – 209	38	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6R2K7-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 02A28B63B6EFD9EC	FASTA	310	33,830
	10 20 30 40 50 60 MAMFSWTSSE AINGMYIPSA LLIFGTAIVK KEWLPYAVAL AAILSGGKVF SNRQRKVLNP 70 80 90 100 110 120 TEFQNFELKE KTIVSHNVAI YRFALPRPTD ILGLPIGQHI SLAATIEGQT KEIMRSYTPI 130 140 150 160 170 180 SSDQEAGYFD LLVKAYPQGN ISKHLAGLRI GQTMKVRGPK GAMVYTPNMV KKIGMIAGGT 190 200 210 220 230 240 GITPMLQIIK AIIRGRPRNG GNDTTQVDLI FANVNPDDIL LKDELDQLAK EDDGFRVFYV 250 260 270 280 290 300 LNNPPEGWEG GVGFVTPDMI RAKLPAAAPD TKVLICGPPP MVSAMKKATE SLGFKKAGLV 310 SKLEDQVFCF

References

[1]

"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed: 19717792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476657 Genomic DNA. Translation: EDN07334.1.
RefSeq	XP_001541767.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5447979.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCB5R_AJECN

Accession

Primary (citable) accession number: A6R2K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)