Reviewed, UniProtKB/Swiss-Prot A6R1T7 (MCR1_AJECN)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 2
    EC=1.6.2.2
Alternative name(s):
    Mitochondrial cytochrome b reductase

Gene names

Name:	MCR1
ORF Names:	HCAG_03595

Organism

Ajellomyces capsulata (strain NAm1 / WU24) (Darling's disease fungus) (Histoplasma capsulatum)

Taxonomic identifier

339724 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › Ajellomycetaceae › Ajellomyces

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May mediate the reduction of outer membrane cytochrome b5 By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	NADH-cytochrome b5 reductase 2		PRO_0000330168
Regions
	Transmembrane	31 – 47	17	Potential
	Domain	70 – 178	109	FAD-binding FR-type
	Nucleotide binding	181 – 216	36	FAD By similarity
	Compositional bias	45 – 48	4	Poly-Tyr

Sequences

	Sequence		Length	Mass (Da)
	A6R1T7-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 54489BF296707A0F	FASTA	324	36,615
	10 20 30 40 50 60 MFARYTFRCS QPLAQSVRKY SSEAPAKSSR IPLIGGITLA AGAGYYYYWQ TTSAKSEPKE 70 80 90 100 110 120 RSTVFKGGDQ GWIGLKLAHI DNVNHNVKKL RFEFEDPESV SGLHIASALL TKYKGLTDEK 130 140 150 160 170 180 PTIRPYTPVS DEGMWASLGY LDLLVKRYPN GPMSNHLHNM AVGQRLDFKG PLPKYPWEPS 190 200 210 220 230 240 KHDHICLIAG GTGITPMYQL VRKIFSNPED KTKVTLVFAN VTEEDILLRK EFEHLENTYP 250 260 270 280 290 300 RRFRAFYTLD KPPKNWAQGT GFITKDLLKT VLPEPKTENI KIFVCGPPAM YKAISGQKVS 310 320 PKDQGELSGI LKELGYSKEQ VYKF

References

[1]

"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed: 19717792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476657 Genomic DNA. Translation: EDN07064.1.
RefSeq	XP_001541497.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5447518.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCR1_AJECN

Accession

Primary (citable) accession number: A6R1T7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)