A6QX86 (KEX1_AJECN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pheromone-processing carboxypeptidase KEX1
EC=3.4.16.6

Alternative name(s):
Carboxypeptidase D

Gene names

Name:	KEX1
ORF Names:	HCAG_01993

Organism

Ajellomyces capsulata (strain NAm1 / WU24) (Darling's disease fungus) (Histoplasma capsulatum) [Complete proteome]

Taxonomic identifier

339724 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Onygenales › Ajellomycetaceae › Ajellomyces › Ajellomyces capsulatus

Protein attributes

Sequence length	634 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from protein precursors. Promotes cell fusion and is involved in the programmed cell death By similarity.
Catalytic activity	Preferential release of a C-terminal arginine or lysine residue.
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Belongs to the peptidase S10 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Golgi apparatus Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Carboxypeptidase Hydrolase Protease
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	serine-type carboxypeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Chain	34 – 634	601	Pheromone-processing carboxypeptidase KEX1		PRO_0000411896
Regions
	Topological domain	34 – 517	484	Lumenal Potential
	Transmembrane	518 – 538	21	Helical; Potential
	Topological domain	539 – 634	96	Cytoplasmic Potential
	Compositional bias	560 – 565	6	Poly-Ser
Sites
	Active site	185	1	By similarity
	Active site	384	1	By similarity
	Active site	446	1	By similarity
Amino acid modifications
	Glycosylation	118	1	N-linked (GlcNAc...) Potential
	Glycosylation	328	1	N-linked (GlcNAc...) Potential
	Glycosylation	435	1	N-linked (GlcNAc...) Potential
	Glycosylation	443	1	N-linked (GlcNAc...) Potential
	Glycosylation	495	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A6QX86 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 40C8F861DEA7CEBD	FASTA	634	70,866
	10 20 30 40 50 60 MGFSEARAYR AFGGRSTWLT VFLALANSLA VSAKCAADYF VDSLPGQPDS PQVQMHAGHI 70 80 90 100 110 120 EINHKTSANL FFWHVANQHI ADKPRTVIWL NGGPGCSSED GALMEIGPYR VTNDHLLNHT 130 140 150 160 170 180 DGSWDEFANL LFVDQPVGTG FSYVSTGAYV SELGEMASQF VTFLEKWFEL FPHYEKNDLY 190 200 210 220 230 240 FAGESYAGQY IPYIARAILD RNKKGESLTR WKLKGILIGN GWISPRHQYL SYLPYAYQEG 250 260 270 280 290 300 IIQGGTDSSS RVEAKLSKCL NKLNVEDSTG TVQISACEEV LQAIIDETHK GNRCINMYDI 310 320 330 340 350 360 RLTDEYSACG MNWPPDLENM APYLRFKNVT KALHINSDKQ TGWSECSGAV SGHFRALKSK 370 380 390 400 410 420 PSVELLPGLL EEGLPILLFS GQKDMICNHI GNEDLIKDMK WSGGTGFELS PGVWAPRQDW 430 440 450 460 470 480 IFEGESAGFY QQARNLTYVL FYNASHMVPF NYPPRSREML DRFIGVDIAD IGGNPADSRI 490 500 510 520 530 540 DGEKGPATSV RAHPNSTAAA EREKEKVKSA MWKAYYKSGE VALIVVAIAA VIWGVFIWRS 550 560 570 580 590 600 RQKHQDRGYE FRGIYPMLGS SSSGSLPRYS NKRGRRGHDV EAVNIYEAEL DERPSRVVSA 610 620 630 RSSREQEPYV VGDEDGSDVD DDTSDERKRL VDKP

References

[1]

"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NAm1 / WU24.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476655 Genomic DNA. Translation: EDN04128.1.
RefSeq	XP_001544946.1. XM_001544896.1.
3D structure databases
ProteinModelPortal	A6QX86.
ModBase	Search...
Protein family/group databases
MEROPS	S10.007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5449542.
KEGG	aje:HCAG_01993.
Family and domain databases
InterPro	IPR001563. Peptidase_S10. [Graphical view]
PANTHER	PTHR11802. PTHR11802. 1 hit.
Pfam	PF00450. Peptidase_S10. 1 hit. [Graphical view]
PRINTS	PR00724. CRBOXYPTASEC.
PROSITE	PS00560. CARBOXYPEPT_SER_HIS. False negative. PS00131. CARBOXYPEPT_SER_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KEX1_AJECN

Accession

Primary (citable) accession number: A6QX86

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	August 21, 2007
Last modified:	March 6, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program