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A6QR55

- UBP4_BOVIN

UniProt

A6QR55 - UBP4_BOVIN

Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene

USP4

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei311 – 3111NucleophilePROSITE-ProRule annotation
    Metal bindingi461 – 4611ZincBy similarity
    Metal bindingi464 – 4641ZincBy similarity
    Metal bindingi799 – 7991ZincBy similarity
    Metal bindingi802 – 8021ZincBy similarity
    Active sitei881 – 8811Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ubiquitin-specific protease activity Source: UniProtKB
    3. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. negative regulation of protein ubiquitination Source: UniProtKB
    2. protein deubiquitination Source: UniProtKB
    3. protein localization to cell surface Source: UniProtKB
    4. regulation of protein stability Source: UniProtKB
    5. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 4
    Ubiquitin thioesterase 4
    Ubiquitin-specific-processing protease 4
    Gene namesi
    Name:USP4
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 22

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 963963Ubiquitin carboxyl-terminal hydrolase 4PRO_0000301668Add
    BLAST

    Post-translational modificationi

    Monoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated By similarity.By similarity

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PRIDEiA6QR55.

    Interactioni

    Subunit structurei

    Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with RB1, RBL1 and RBL2 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000015787.

    Structurei

    3D structure databases

    ProteinModelPortaliA6QR55.
    SMRiA6QR55. Positions 1-124.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 122112DUSPPROSITE-ProRule annotationAdd
    BLAST
    Domaini142 – 22685Ubiquitin-like 1Add
    BLAST
    Domaini302 – 923622USPAdd
    BLAST
    Domaini483 – 57189Ubiquitin-like 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni405 – 4073Necessary for interaction with RBL2By similarity
    Regioni459 – 4635Necessary for interaction with RB1 and RBL2By similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi133 – 1419Nuclear export signalBy similarity
    Motifi767 – 7726Nuclear localization signalBy similarity

    Domaini

    The Ubiquitin-like domain 2 inserts into the catalytic domain and competes with the ubiquitin substrate, partially inhibiting DUB activity.By similarity

    Sequence similaritiesi

    Belongs to the peptidase C19 family. USP4 subfamily.Curated
    Contains 1 DUSP domain.PROSITE-ProRule annotation
    Contains 2 ubiquitin-like domains.Curated
    Contains 1 USP domain.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5560.
    GeneTreeiENSGT00670000097750.
    HOGENOMiHOG000264375.
    HOVERGENiHBG000864.
    InParanoidiA6QR55.
    KOiK11835.
    OMAiHCRPTQY.
    OrthoDBiEOG77Q4VW.
    TreeFamiTF106276.

    Family and domain databases

    Gene3Di3.30.2230.10. 1 hit.
    InterProiIPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028135. Ub_USP-typ.
    IPR028889. UCH/PAN2.
    IPR028134. USP.
    [Graphical view]
    PANTHERiPTHR24006:SF360. PTHR24006:SF360. 1 hit.
    PfamiPF06337. DUSP. 1 hit.
    PF14836. Ubiquitin_3. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM00695. DUSP. 1 hit.
    [Graphical view]
    SUPFAMiSSF143791. SSF143791. 1 hit.
    PROSITEiPS51283. DUSP. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6QR55-1 [UniParc]FASTAAdd to Basket

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    MAEGGGYRER PDAETQKSEL GALMRTTLQR GAQWYLIDSR WFKQWKKYVG    50
    FDSWDMYNVG EHNLYPGPID NSGLFSDPES QTLKEHLIDE LDYVLVPAEA 100
    WNKLLNWYGC VEGQQPIVRK VVEHGLFVKH CKVEVYLLEL KLCENSDPTN 150
    VLSCHFSKAD TIATIEKEMR KLFNIPAERE TRLWNKYMSN TYEQLSKLDN 200
    TVQDAGLYQG QVLVIEPQNE DGTWPRQTQQ SKSSTAPSRN FTTSPKSSAS 250
    PYSSVSASPI ANGDSTNTSG MHSSGVSRGG SGFSASYNCQ ESPLTHVQPG 300
    LCGLGNLGNT CFMNSALQCL SNTAPLTDYF LKDEYEAEIN RDNPLGMKGE 350
    IAEAYAELIK QMWSGRDAHV APRMFKTQVG RFAPQFSGYQ QQDSQELLAF 400
    LLDGLHEDLN RVKKKPYLEL KDANGRPDAV VAKEAWENHR LRNDSVIVDT 450
    FHGLFKSTLV CPECAKVSVT FDPFCYLTLP LPLKKDRVME IFLVPADPRC 500
    RPTQYRVVVP LMGAVSDLCE ALSKLSGIAA ENMVVTDVYN HRFHKIFQMD 550
    EGLNHIMPRD DIFVYEVCST SPDGSECVTL PVYFRERKSR PSSTSTGAVL 600
    YGQPLLVSVP KHKLTLESLY QAVCERISRY IKQPLPDESG SSPLELGACN 650
    GSRSGCAGED EEEMEHQEEG REQLSETEGS GDDEPGSDHG EATQKKNKGR 700
    PCPRRLFTFS LVNSYGTADI NSLATDGKLL KLNSRSTLAI DWDSETRSCY 750
    YNEQESETYE KHVSMLQPQK KKKTAVALRD CIELFTTMET LGEHDPWYCP 800
    NCKKHQQATK KFDLWSLPKI LVVHLKRFSY NRYWRDKLDT VVEFPVRGLN 850
    MSEFVCDPSA RPYVYDLIAV SNHYGAMGVG HYTAYAKNKL NGKWYYFDDS 900
    NVSLACEDQI VTKAAYVLFY QRRDDEFHKT PSLSFPGSSD GGARPSSSQQ 950
    GTGDDETYSM DTN 963
    Length:963
    Mass (Da):108,510
    Last modified:August 21, 2007 - v1
    Checksum:i6D132FFE8C371A60
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC150120 mRNA. Translation: AAI50121.1.
    RefSeqiNP_001093789.1. NM_001100319.1.
    UniGeneiBt.52457.

    Genome annotation databases

    EnsembliENSBTAT00000015787; ENSBTAP00000015787; ENSBTAG00000011899.
    GeneIDi508042.
    KEGGibta:508042.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC150120 mRNA. Translation: AAI50121.1 .
    RefSeqi NP_001093789.1. NM_001100319.1.
    UniGenei Bt.52457.

    3D structure databases

    ProteinModelPortali A6QR55.
    SMRi A6QR55. Positions 1-124.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000015787.

    Proteomic databases

    PRIDEi A6QR55.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000015787 ; ENSBTAP00000015787 ; ENSBTAG00000011899 .
    GeneIDi 508042.
    KEGGi bta:508042.

    Organism-specific databases

    CTDi 7375.

    Phylogenomic databases

    eggNOGi COG5560.
    GeneTreei ENSGT00670000097750.
    HOGENOMi HOG000264375.
    HOVERGENi HBG000864.
    InParanoidi A6QR55.
    KOi K11835.
    OMAi HCRPTQY.
    OrthoDBi EOG77Q4VW.
    TreeFami TF106276.

    Miscellaneous databases

    NextBioi 20868329.

    Family and domain databases

    Gene3Di 3.30.2230.10. 1 hit.
    InterProi IPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028135. Ub_USP-typ.
    IPR028889. UCH/PAN2.
    IPR028134. USP.
    [Graphical view ]
    PANTHERi PTHR24006:SF360. PTHR24006:SF360. 1 hit.
    Pfami PF06337. DUSP. 1 hit.
    PF14836. Ubiquitin_3. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM00695. DUSP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF143791. SSF143791. 1 hit.
    PROSITEi PS51283. DUSP. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Basal ganglia.

    Entry informationi

    Entry nameiUBP4_BOVIN
    AccessioniPrimary (citable) accession number: A6QR55
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3