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A6QR55

- UBP4_BOVIN

UniProt

A6QR55 - UBP4_BOVIN

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Protein
Ubiquitin carboxyl-terminal hydrolase 4
Gene
USP4
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei311 – 3111Nucleophile By similarity
Metal bindingi461 – 4611Zinc By similarity
Metal bindingi464 – 4641Zinc By similarity
Metal bindingi799 – 7991Zinc By similarity
Metal bindingi802 – 8021Zinc By similarity
Active sitei881 – 8811Proton acceptor By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ubiquitin thiolesterase activity Source: UniProtKB
  3. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. negative regulation of protein ubiquitination Source: UniProtKB
  2. protein deubiquitination Source: UniProtKB
  3. protein localization to cell surface Source: UniProtKB
  4. regulation of protein stability Source: UniProtKB
  5. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene namesi
Name:USP4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 22

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 963963Ubiquitin carboxyl-terminal hydrolase 4
PRO_0000301668Add
BLAST

Post-translational modificationi

Monoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated By similarity.

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiA6QR55.

Interactioni

Subunit structurei

Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with RB1, RBL1 and RBL2 By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015787.

Structurei

3D structure databases

ProteinModelPortaliA6QR55.
SMRiA6QR55. Positions 1-124.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 122112DUSP
Add
BLAST
Domaini142 – 22685Ubiquitin-like 1
Add
BLAST
Domaini302 – 923622USP
Add
BLAST
Domaini483 – 57189Ubiquitin-like 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni405 – 4073Necessary for interaction with RBL2 By similarity
Regioni459 – 4635Necessary for interaction with RB1 and RBL2 By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi133 – 1419Nuclear export signal By similarity
Motifi767 – 7726Nuclear localization signal By similarity

Domaini

The Ubiquitin-like domain 2 inserts into the catalytic domain and competes with the ubiquitin substrate, partially inhibiting DUB activity By similarity.

Sequence similaritiesi

Contains 1 DUSP domain.
Contains 1 USP domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00670000097750.
HOGENOMiHOG000264375.
HOVERGENiHBG000864.
InParanoidiA6QR55.
KOiK11835.
OMAiHCRPTQY.
OrthoDBiEOG77Q4VW.
TreeFamiTF106276.

Family and domain databases

Gene3Di3.30.2230.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028135. Ub_USP-typ.
IPR028889. UCH/PAN2.
IPR028134. USP.
[Graphical view]
PANTHERiPTHR24006:SF360. PTHR24006:SF360. 1 hit.
PfamiPF06337. DUSP. 1 hit.
PF14836. Ubiquitin_3. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 1 hit.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 1 hit.
PROSITEiPS51283. DUSP. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QR55-1 [UniParc]FASTAAdd to Basket

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MAEGGGYRER PDAETQKSEL GALMRTTLQR GAQWYLIDSR WFKQWKKYVG    50
FDSWDMYNVG EHNLYPGPID NSGLFSDPES QTLKEHLIDE LDYVLVPAEA 100
WNKLLNWYGC VEGQQPIVRK VVEHGLFVKH CKVEVYLLEL KLCENSDPTN 150
VLSCHFSKAD TIATIEKEMR KLFNIPAERE TRLWNKYMSN TYEQLSKLDN 200
TVQDAGLYQG QVLVIEPQNE DGTWPRQTQQ SKSSTAPSRN FTTSPKSSAS 250
PYSSVSASPI ANGDSTNTSG MHSSGVSRGG SGFSASYNCQ ESPLTHVQPG 300
LCGLGNLGNT CFMNSALQCL SNTAPLTDYF LKDEYEAEIN RDNPLGMKGE 350
IAEAYAELIK QMWSGRDAHV APRMFKTQVG RFAPQFSGYQ QQDSQELLAF 400
LLDGLHEDLN RVKKKPYLEL KDANGRPDAV VAKEAWENHR LRNDSVIVDT 450
FHGLFKSTLV CPECAKVSVT FDPFCYLTLP LPLKKDRVME IFLVPADPRC 500
RPTQYRVVVP LMGAVSDLCE ALSKLSGIAA ENMVVTDVYN HRFHKIFQMD 550
EGLNHIMPRD DIFVYEVCST SPDGSECVTL PVYFRERKSR PSSTSTGAVL 600
YGQPLLVSVP KHKLTLESLY QAVCERISRY IKQPLPDESG SSPLELGACN 650
GSRSGCAGED EEEMEHQEEG REQLSETEGS GDDEPGSDHG EATQKKNKGR 700
PCPRRLFTFS LVNSYGTADI NSLATDGKLL KLNSRSTLAI DWDSETRSCY 750
YNEQESETYE KHVSMLQPQK KKKTAVALRD CIELFTTMET LGEHDPWYCP 800
NCKKHQQATK KFDLWSLPKI LVVHLKRFSY NRYWRDKLDT VVEFPVRGLN 850
MSEFVCDPSA RPYVYDLIAV SNHYGAMGVG HYTAYAKNKL NGKWYYFDDS 900
NVSLACEDQI VTKAAYVLFY QRRDDEFHKT PSLSFPGSSD GGARPSSSQQ 950
GTGDDETYSM DTN 963
Length:963
Mass (Da):108,510
Last modified:August 21, 2007 - v1
Checksum:i6D132FFE8C371A60
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC150120 mRNA. Translation: AAI50121.1.
RefSeqiNP_001093789.1. NM_001100319.1.
UniGeneiBt.52457.

Genome annotation databases

EnsembliENSBTAT00000015787; ENSBTAP00000015787; ENSBTAG00000011899.
GeneIDi508042.
KEGGibta:508042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC150120 mRNA. Translation: AAI50121.1 .
RefSeqi NP_001093789.1. NM_001100319.1.
UniGenei Bt.52457.

3D structure databases

ProteinModelPortali A6QR55.
SMRi A6QR55. Positions 1-124.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000015787.

Proteomic databases

PRIDEi A6QR55.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000015787 ; ENSBTAP00000015787 ; ENSBTAG00000011899 .
GeneIDi 508042.
KEGGi bta:508042.

Organism-specific databases

CTDi 7375.

Phylogenomic databases

eggNOGi COG5560.
GeneTreei ENSGT00670000097750.
HOGENOMi HOG000264375.
HOVERGENi HBG000864.
InParanoidi A6QR55.
KOi K11835.
OMAi HCRPTQY.
OrthoDBi EOG77Q4VW.
TreeFami TF106276.

Miscellaneous databases

NextBioi 20868329.

Family and domain databases

Gene3Di 3.30.2230.10. 1 hit.
InterProi IPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028135. Ub_USP-typ.
IPR028889. UCH/PAN2.
IPR028134. USP.
[Graphical view ]
PANTHERi PTHR24006:SF360. PTHR24006:SF360. 1 hit.
Pfami PF06337. DUSP. 1 hit.
PF14836. Ubiquitin_3. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view ]
SMARTi SM00695. DUSP. 1 hit.
[Graphical view ]
SUPFAMi SSF143791. SSF143791. 1 hit.
PROSITEi PS51283. DUSP. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Basal ganglia.

Entry informationi

Entry nameiUBP4_BOVIN
AccessioniPrimary (citable) accession number: A6QR55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: August 21, 2007
Last modified: June 11, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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