Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A6QR55

- UBP4_BOVIN

UniProt

A6QR55 - UBP4_BOVIN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene

USP4

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei311 – 3111NucleophilePROSITE-ProRule annotation
Metal bindingi461 – 4611ZincBy similarity
Metal bindingi464 – 4641ZincBy similarity
Metal bindingi799 – 7991ZincBy similarity
Metal bindingi802 – 8021ZincBy similarity
Active sitei881 – 8811Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ubiquitin-specific protease activity Source: UniProtKB
  3. ubiquitin thiolesterase activity Source: UniProtKB

GO - Biological processi

  1. negative regulation of protein ubiquitination Source: UniProtKB
  2. protein deubiquitination Source: UniProtKB
  3. protein localization to cell surface Source: UniProtKB
  4. regulation of protein stability Source: UniProtKB
  5. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene namesi
Name:USP4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 22

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
  3. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 963963Ubiquitin carboxyl-terminal hydrolase 4PRO_0000301668Add
BLAST

Post-translational modificationi

Monoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated (By similarity).By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiA6QR55.

Interactioni

Subunit structurei

Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with RB1, RBL1 and RBL2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015787.

Structurei

3D structure databases

ProteinModelPortaliA6QR55.
SMRiA6QR55. Positions 1-124.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 122112DUSPPROSITE-ProRule annotationAdd
BLAST
Domaini142 – 22685Ubiquitin-like 1Add
BLAST
Domaini302 – 923622USPAdd
BLAST
Domaini483 – 57189Ubiquitin-like 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni405 – 4073Necessary for interaction with RBL2By similarity
Regioni459 – 4635Necessary for interaction with RB1 and RBL2By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi133 – 1419Nuclear export signalBy similarity
Motifi767 – 7726Nuclear localization signalBy similarity

Domaini

The Ubiquitin-like domain 2 inserts into the catalytic domain and competes with the ubiquitin substrate, partially inhibiting DUB activity.By similarity

Sequence similaritiesi

Belongs to the peptidase C19 family. USP4 subfamily.Curated
Contains 1 DUSP domain.PROSITE-ProRule annotation
Contains 2 ubiquitin-like domains.Curated
Contains 1 USP domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00670000097750.
HOGENOMiHOG000264375.
HOVERGENiHBG000864.
InParanoidiA6QR55.
KOiK11835.
OMAiHCRPTQY.
OrthoDBiEOG77Q4VW.
TreeFamiTF106276.

Family and domain databases

Gene3Di3.30.2230.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028135. Ub_USP-typ.
IPR028889. UCH/PAN2.
IPR028134. USP.
[Graphical view]
PANTHERiPTHR24006:SF360. PTHR24006:SF360. 1 hit.
PfamiPF06337. DUSP. 1 hit.
PF14836. Ubiquitin_3. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 1 hit.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 1 hit.
PROSITEiPS51283. DUSP. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QR55-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEGGGYRER PDAETQKSEL GALMRTTLQR GAQWYLIDSR WFKQWKKYVG
60 70 80 90 100
FDSWDMYNVG EHNLYPGPID NSGLFSDPES QTLKEHLIDE LDYVLVPAEA
110 120 130 140 150
WNKLLNWYGC VEGQQPIVRK VVEHGLFVKH CKVEVYLLEL KLCENSDPTN
160 170 180 190 200
VLSCHFSKAD TIATIEKEMR KLFNIPAERE TRLWNKYMSN TYEQLSKLDN
210 220 230 240 250
TVQDAGLYQG QVLVIEPQNE DGTWPRQTQQ SKSSTAPSRN FTTSPKSSAS
260 270 280 290 300
PYSSVSASPI ANGDSTNTSG MHSSGVSRGG SGFSASYNCQ ESPLTHVQPG
310 320 330 340 350
LCGLGNLGNT CFMNSALQCL SNTAPLTDYF LKDEYEAEIN RDNPLGMKGE
360 370 380 390 400
IAEAYAELIK QMWSGRDAHV APRMFKTQVG RFAPQFSGYQ QQDSQELLAF
410 420 430 440 450
LLDGLHEDLN RVKKKPYLEL KDANGRPDAV VAKEAWENHR LRNDSVIVDT
460 470 480 490 500
FHGLFKSTLV CPECAKVSVT FDPFCYLTLP LPLKKDRVME IFLVPADPRC
510 520 530 540 550
RPTQYRVVVP LMGAVSDLCE ALSKLSGIAA ENMVVTDVYN HRFHKIFQMD
560 570 580 590 600
EGLNHIMPRD DIFVYEVCST SPDGSECVTL PVYFRERKSR PSSTSTGAVL
610 620 630 640 650
YGQPLLVSVP KHKLTLESLY QAVCERISRY IKQPLPDESG SSPLELGACN
660 670 680 690 700
GSRSGCAGED EEEMEHQEEG REQLSETEGS GDDEPGSDHG EATQKKNKGR
710 720 730 740 750
PCPRRLFTFS LVNSYGTADI NSLATDGKLL KLNSRSTLAI DWDSETRSCY
760 770 780 790 800
YNEQESETYE KHVSMLQPQK KKKTAVALRD CIELFTTMET LGEHDPWYCP
810 820 830 840 850
NCKKHQQATK KFDLWSLPKI LVVHLKRFSY NRYWRDKLDT VVEFPVRGLN
860 870 880 890 900
MSEFVCDPSA RPYVYDLIAV SNHYGAMGVG HYTAYAKNKL NGKWYYFDDS
910 920 930 940 950
NVSLACEDQI VTKAAYVLFY QRRDDEFHKT PSLSFPGSSD GGARPSSSQQ
960
GTGDDETYSM DTN
Length:963
Mass (Da):108,510
Last modified:August 21, 2007 - v1
Checksum:i6D132FFE8C371A60
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC150120 mRNA. Translation: AAI50121.1.
RefSeqiNP_001093789.1. NM_001100319.1.
UniGeneiBt.52457.

Genome annotation databases

EnsembliENSBTAT00000015787; ENSBTAP00000015787; ENSBTAG00000011899.
GeneIDi508042.
KEGGibta:508042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC150120 mRNA. Translation: AAI50121.1 .
RefSeqi NP_001093789.1. NM_001100319.1.
UniGenei Bt.52457.

3D structure databases

ProteinModelPortali A6QR55.
SMRi A6QR55. Positions 1-124.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000015787.

Proteomic databases

PRIDEi A6QR55.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000015787 ; ENSBTAP00000015787 ; ENSBTAG00000011899 .
GeneIDi 508042.
KEGGi bta:508042.

Organism-specific databases

CTDi 7375.

Phylogenomic databases

eggNOGi COG5560.
GeneTreei ENSGT00670000097750.
HOGENOMi HOG000264375.
HOVERGENi HBG000864.
InParanoidi A6QR55.
KOi K11835.
OMAi HCRPTQY.
OrthoDBi EOG77Q4VW.
TreeFami TF106276.

Miscellaneous databases

NextBioi 20868329.

Family and domain databases

Gene3Di 3.30.2230.10. 1 hit.
InterProi IPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028135. Ub_USP-typ.
IPR028889. UCH/PAN2.
IPR028134. USP.
[Graphical view ]
PANTHERi PTHR24006:SF360. PTHR24006:SF360. 1 hit.
Pfami PF06337. DUSP. 1 hit.
PF14836. Ubiquitin_3. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view ]
SMARTi SM00695. DUSP. 1 hit.
[Graphical view ]
SUPFAMi SSF143791. SSF143791. 1 hit.
PROSITEi PS51283. DUSP. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Basal ganglia.

Entry informationi

Entry nameiUBP4_BOVIN
AccessioniPrimary (citable) accession number: A6QR55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: August 21, 2007
Last modified: October 29, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3