A6QR55 (UBP4_BOVIN) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 44.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Ubiquitin carboxyl-terminal hydrolase 4 EC=3.4.19.12 Alternative name(s): Deubiquitinating enzyme 4 Ubiquitin thioesterase 4 Ubiquitin-specific-processing protease 4 | ||
| Gene names |
| ||
| Organism | Bos taurus (Bovine) [Reference proteome] | ||
| Taxonomic identifier | 9913 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Protein attributes
| Sequence length | 963 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER By similarity. |
| Catalytic activity | Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). |
| Subunit structure | Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with RB1, RBL1 and RBL2 By similarity. |
| Subcellular location | Cytoplasm By similarity. Nucleus By similarity. Note: Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type By similarity. |
| Domain | The Ubiquitin-like domain 2 inserts into the catalytic domain and competes with the ubiquitin substrate, partially inhibiting DUB activity By similarity. |
| Post-translational modification | Monoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated By similarity. |
| Sequence similarities | Belongs to the peptidase C19 family. USP4 subfamily. Contains 1 DUSP domain. Contains 2 ubiquitin-like domains. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 963 | 963 | Ubiquitin carboxyl-terminal hydrolase 4 | PRO_0000301668 | |||||
Regions | |||||||||
| Domain | 11 – 122 | 112 | DUSP | ||||||
| Domain | 142 – 226 | 85 | Ubiquitin-like 1 | ||||||
| Domain | 483 – 571 | 89 | Ubiquitin-like 2 | ||||||
| Region | 405 – 407 | 3 | Necessary for interaction with RBL2 By similarity | ||||||
| Region | 459 – 463 | 5 | Necessary for interaction with RB1 and RBL2 By similarity | ||||||
| Motif | 133 – 141 | 9 | Nuclear export signal By similarity | ||||||
| Motif | 767 – 772 | 6 | Nuclear localization signal By similarity | ||||||
Sites | |||||||||
| Active site | 311 | 1 | Nucleophile By similarity | ||||||
| Active site | 881 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 461 | 1 | Zinc By similarity | ||||||
| Metal binding | 464 | 1 | Zinc By similarity | ||||||
| Metal binding | 799 | 1 | Zinc By similarity | ||||||
| Metal binding | 802 | 1 | Zinc By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 680 | 1 | Phosphoserine By similarity | ||||||
Sequences
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References
| [1] | NIH - Mammalian Gene Collection (MGC) project Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Basal ganglia. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BC150120 mRNA. Translation: AAI50121.1. |
| IPI | IPI00697204. |
| RefSeq | NP_001093789.1. NM_001100319.1. |
| UniGene | Bt.52457. |
3D structure databases | |
| ProteinModelPortal | A6QR55. |
| SMR | A6QR55. Positions 1-124. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 9913.ENSBTAP00000015787. |
Proteomic databases | |
| PRIDE | A6QR55. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSBTAT00000015787; ENSBTAP00000015787; ENSBTAG00000011899. |
| GeneID | 508042. |
| KEGG | bta:508042. |
Organism-specific databases | |
| CTD | 7375. |
Phylogenomic databases | |
| eggNOG | COG5560. |
| GeneTree | ENSGT00670000097750. |
| HOGENOM | HOG000264375. |
| HOVERGEN | HBG000864. |
| InParanoid | A6QR55. |
| KO | K11835. |
| OMA | CERISRY. |
| OrthoDB | EOG4C5CHR. |
Family and domain databases | |
| InterPro | IPR006615. Pept_C19_DUSP. IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view] |
| Pfam | PF06337. DUSP. 1 hit. PF00443. UCH. 1 hit. [Graphical view] |
| SMART | SM00695. DUSP. 1 hit. [Graphical view] |
| PROSITE | PS51283. DUSP. 1 hit. PS00299. UBIQUITIN_1. False negative. PS50053. UBIQUITIN_2. False negative. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 20868329. |
Entry information
| Entry name | UBP4_BOVIN | ||||||||
| Accession | Primary (citable) accession number: A6QR55 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |