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Protein

SMC5-SMC6 complex localization factor protein 1

Gene

SLF1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance. The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks. Promotes the recruitment of SLF2 and the SMC5-SMC6 complex to DNA lesions.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
SMC5-SMC6 complex localization factor protein 1By similarity
Alternative name(s):
Ankyrin repeat domain-containing protein 32
BRCT domain-containing protein 1
Gene namesi
Name:SLF1By similarity
Synonyms:ANKRD32, BRCTD1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

  • Note: Relocalizes with RAD18 to nuclear foci in response to DNA damage. Colocalizes with RAD18 in the nucleus and to centrosomes. Associates with chromatin. Accumulates with RAD18 and the SMC5-SMC6 complex at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003423401 – 1055SMC5-SMC6 complex localization factor protein 1Add BLAST1055

Proteomic databases

PaxDbiA6QR20.

Interactioni

Subunit structurei

Interacts (via N-terminus) with SLF2; this interaction links RAD18 to the SMC5-SMC6 complex. Interacts (via BRCT domains) with RAD18; this interaction occurs in a SLF2-independent manner. Interacts with SMC6. Interacts (via BRCT domains) with RAD18 (via C-terminus and phosphorylated form); this interaction is required for efficient repair of UV-induced DNA damage.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000008026.

Structurei

3D structure databases

ProteinModelPortaliA6QR20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 80BRCT 1Add BLAST79
Domaini121 – 199BRCT 2Add BLAST79
Repeati804 – 834ANK 1Add BLAST31
Repeati838 – 867ANK 2Add BLAST30
Repeati872 – 901ANK 3Add BLAST30

Domaini

BRCT domains are necessary for its targeting to ionizing radiation-induced nuclear foci.By similarity

Sequence similaritiesi

Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 2 BRCT domains.Curated

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
KOG1929. Eukaryota.
ENOG410XPFH. LUCA.
HOGENOMiHOG000033961.
HOVERGENiHBG103143.
InParanoidiA6QR20.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di1.25.40.20. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001357. BRCT_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF16770. RTT107_BRCT_5. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QR20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDDAPKHII QMTGFKVEEK EALGKLLLKL DCTFIKSEKY KNCTHLIAER
60 70 80 90 100
LCKSEKFLAA CAAGKWVLTK DYIIHSAQSG RWLDETTYEW GYKIEKDSHY
110 120 130 140 150
SPQMQSAPKR WREELKRTGA PGAFHKWKVV LLVRADKRSD SLVRVLEAGK
160 170 180 190 200
ANVILPKNSP TGITHVIASN ARIKAEQEKD DFKAPFYPIQ YLEDFLLEKE
210 220 230 240 250
IHNDEDSQTN STWKNHSSQE KSNDFRENMG FLEMKGTLKE TMCRTQKEMK
260 270 280 290 300
NHDEDVTISS ILTEHQSKER FRDSRKNLKF VKMRNALGRH TYRNQEMKKK
310 320 330 340 350
DEDIQSIYTL RKKRKKEKER DSRKDIEHDR STLRKHIYRD QKERKNSVFA
360 370 380 390 400
GHAKESKTKD IRTNVDIVDL KNALRKHIYR AQAVRYRGIR IDKQPAYNVE
410 420 430 440 450
VKNAEFPRGI LNLIESLIEG QFFKEAIEEL SSLQAHYIPP VYLLHALLEN
460 470 480 490 500
ILQDNIDTFS GRYFHILSAL LHLHPPWKSP AMSTYYLELF QCPTCMKGTW
510 520 530 540 550
SLIEVLIRSC LFNESFCHQI SENIIGSKVL HLTLLKFFFN LIESEVRHLS
560 570 580 590 600
QKLYDWSDSQ SLKITGKAVL LEIFWSGNET SGLLTKPVNM LLEWTIYSHK
610 620 630 640 650
EKCKSNDVFR HELAYLLTGI LGAAIDYWIV LGLNMGRNVM RHMSDDVGSY
660 670 680 690 700
VSLSCDDFSS QDLEIFISSF SSSWLQMFVA EAVFKKLCLQ NSISISSEPL
710 720 730 740 750
SLQKMVYSYL PALGKTGVRG TRKMQKPKKI GLRPCFESQR ALIMLNGAKQ
760 770 780 790 800
KQGEGLPEIP ELNLAKCSSS LKRLKKKSEG ELSCSKENCP SLVTKINFHK
810 820 830 840 850
TNLKGETALH RACINNQVDR LILLLSMPGI DINVKDNAGW TPLHEACNYG
860 870 880 890 900
NTVCVQEILQ RCPEVDLLTQ VDGVTPLHDA LSNGHVEIGK LLLQHGGPVL
910 920 930 940 950
LQQRNSKGEL PLDYVVSSQI KEELFAITKI EDTVENFHAQ AEKHFYHQQL
960 970 980 990 1000
EFGSFLLSRM LLNFCSIFGL SSESLAFKGL THLSELLIAC QNYKETTSVH
1010 1020 1030 1040 1050
TDWLLDLYAR NIMTLQKLPN ALKELPENVK VCPGVHTEAL LVTLEVMCRS

VTEIS
Length:1,055
Mass (Da):121,017
Last modified:July 1, 2008 - v2
Checksum:i7ED68E2FC51953E9
GO

Sequence cautioni

The sequence AAI50080 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC150079 mRNA. Translation: AAI50080.1. Different initiation.
RefSeqiNP_001095528.2. NM_001102058.2.
UniGeneiBt.37582.

Genome annotation databases

GeneIDi520250.
KEGGibta:520250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC150079 mRNA. Translation: AAI50080.1. Different initiation.
RefSeqiNP_001095528.2. NM_001102058.2.
UniGeneiBt.37582.

3D structure databases

ProteinModelPortaliA6QR20.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000008026.

Proteomic databases

PaxDbiA6QR20.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi520250.
KEGGibta:520250.

Organism-specific databases

CTDi84250.

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
KOG1929. Eukaryota.
ENOG410XPFH. LUCA.
HOGENOMiHOG000033961.
HOVERGENiHBG103143.
InParanoidiA6QR20.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di1.25.40.20. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001357. BRCT_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF16770. RTT107_BRCT_5. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSLF1_BOVIN
AccessioniPrimary (citable) accession number: A6QR20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: November 30, 2016
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.