Reviewed, UniProtKB/Swiss-Prot A6QQV6 (ANM7_BOVIN)

Names and origin

Protein names

Recommended name:
    Protein arginine N-methyltransferase 7
    EC=2.1.1.-
Alternative name(s):
    Histone-arginine N-methyltransferase PRMT7
    EC=2.1.1.125
    [Myelin basic protein]-arginine N-methyltransferase PRMT7
    EC=2.1.1.126

Gene names

Name:

PRMT7

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	695 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3sme2. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3sme2, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone-arginine = S-adenosyl-L-homocysteine + histone-N(omega)-methyl-arginine. S-adenosyl-L-methionine + [myelin basic protein]-arginine = S-adenosyl-L-homocysteine + [myelin basic protein]-N(omega)-methyl-arginine.
Subunit structure	Homodimer and heterodimer. Interacts with CTCFL, PRMT5 and SNRPD3 By similarity.
Subcellular location	Cytoplasm › cytosol By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the protein arginine N-methyltransferase family. PRMT7 subfamily.

Ontologies

Keywords
Biological process	Differentiation Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation during gametogenesis Inferred from sequence or structural similarity. Source: UniProtKB cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW genetic imprinting Inferred from sequence or structural similarity. Source: UniProtKB histone arginine methylation Inferred from sequence or structural similarity. Source: UniProtKB peptidyl-arginine methylation Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW spliceosomal snRNP assembly Inferred from sequence or structural similarity. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	[myelin basic protein]-arginine N-methyltransferase activity Inferred from electronic annotation. Source: EC histone methyltransferase activity (H4-R3 specific) Inferred from sequence or structural similarity. Source: UniProtKB protein-arginine omega-N symmetric methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 695	695	Protein arginine N-methyltransferase 7		PRO_0000373901

Sequences

	Sequence		Length	Mass (Da)
	A6QQV6-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: D668901147C94E95	FASTA	695	78,654
	10 20 30 40 50 60 MKVFCGRANP TTGSVEWLEE DEHYDYHQEI ARSSYADMLH DKDRNMKYYQ GIRAAVSRVK 70 80 90 100 110 120 DRGQKALVLD IGTGTGLLSM MAVTAGADFC YAIEVFKPMA DAAVKIVEKN GFSDKIKVIN 130 140 150 160 170 180 KHSTEVTVGP DGDMPCRANI LITELFDTEL IGEGALPSYE HAHRHLVQAN CEAVPHRATV 190 200 210 220 230 240 YAQLVESRRM WSWNKLFPIR VQTSRGERVI IPPLELERCP GAPSVCDIQL NQVSPADFTI 250 260 270 280 290 300 LSDVLPMFSV DFSKQVSSSA ACHSRQFEPL VSGRAQVVLS WWDIEMDPEG KIKCTMAPSW 310 320 330 340 350 360 AHSDPEELQW RDHWMQCVYF LPQEEPVVQG LALCLVAYHD DYCVWYSLQK TSPEKNGRVH 370 380 390 400 410 420 PVRPVCDCQA HLLWNRPRFG EINDRNRTDQ YIQALRTVLK PDSVCLCVSD GSLLSMLAYH 430 440 450 460 470 480 LGVEQVFTIE NSAVSHRLMK KIFKANHLED KINIIEKRPE LLTPADLEGK KVSLLLGEPF 490 500 510 520 530 540 FTTSLLPWHN LYFWYVRTAV DQHLGPGAVV MPQAASLHVV VVEFRDLWRI RSPCGDCEGF 550 560 570 580 590 600 DVHIMDDMIK RALDFRESKE AEPHPLWEYP CSSLSEPQQI LTFDFRQPVP LQPIHAEGTI 610 620 630 640 650 660 ELRRCGRSHG AVLWMEYHLT ADSTVSTGLL KSAEDEGDCC WNPHCKQAVY FFNTTLDPRA 670 680 690 PPGSSQTVTY TVEFHPHTGD ITMDFTLSDA LDSGC

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Thymus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC150009 mRNA. Translation: AAI50010.1.
IPI	IPI00867369.
RefSeq	NP_001095460.1.
UniGene	Bt.21927
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000012699; ENSBTAP00000012699; ENSBTAG00000009638; Bos taurus. [Genome view]
GeneID	514202.
KEGG	bta:514202.
Organism-specific databases
CTD	514202.
Family and domain databases
InterPro	IPR014644. Arg_N-MeTrfase. [Graphical view]
PIRSF	PIRSF036946. Arg_N-mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ANM7_BOVIN

Accession

Primary (citable) accession number: A6QQV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	August 21, 2007
Last modified:	October 13, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)