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Protein

DNA excision repair protein ERCC-6-like

Gene

ERCC6L

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi122 – 1298ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-2467813. Separation of Sister Chromatids.
R-BTA-2500257. Resolution of Sister Chromatid Cohesion.
R-BTA-5663220. RHO GTPases Activate Formins.
R-BTA-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA excision repair protein ERCC-6-like (EC:3.6.4.12)
Alternative name(s):
ATP-dependent helicase ERCC6-like
Gene namesi
Name:ERCC6L
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome X

Subcellular locationi

  • Chromosomecentromere By similarity
  • Chromosomecentromerekinetochore By similarity

  • Note: Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12421242DNA excision repair protein ERCC-6-likePRO_0000328830Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineBy similarity
Modified residuei755 – 7551PhosphoserineBy similarity
Modified residuei773 – 7731PhosphoserineBy similarity
Modified residuei815 – 8151PhosphothreonineBy similarity
Modified residuei963 – 9631PhosphoserineBy similarity
Modified residuei989 – 9891PhosphoserineBy similarity
Modified residuei998 – 9981PhosphoserineBy similarity
Modified residuei1021 – 10211PhosphoserineBy similarity
Modified residuei1055 – 10551PhosphothreonineBy similarity
Modified residuei1061 – 10611PhosphoserineBy similarity
Modified residuei1090 – 10901PhosphoserineBy similarity
Modified residuei1110 – 11101PhosphoserineBy similarity
Modified residuei1173 – 11731PhosphoserineBy similarity
Modified residuei1180 – 11801PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiA6QQR4.
PRIDEiA6QQR4.

Expressioni

Gene expression databases

BgeeiENSBTAG00000005607.

Interactioni

Subunit structurei

Interacts with PLK1, which phosphorylates it. Both proteins are mutually dependent on each other for correct subcellular localization (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007362.

Structurei

3D structure databases

ProteinModelPortaliA6QQR4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati21 – 5434TPR 1Add
BLAST
Domaini109 – 277169Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini466 – 626161Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Repeati1192 – 122534TPR 2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi228 – 2314DEAH box

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 2 TPR repeats.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG0387. Eukaryota.
ENOG410XP4Z. LUCA.
GeneTreeiENSGT00590000083118.
HOGENOMiHOG000074172.
HOVERGENiHBG107854.
InParanoidiA6QQR4.
KOiK20093.
OMAiICEMPSL.
OrthoDBiEOG091G058Q.
TreeFamiTF332843.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QQR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVSRGFAEA GALSPEQAAS YLRYVKEAKE ATKNGDLEQA LKLFNLAKDI
60 70 80 90 100
FPNEKVMSRI QKIQEALEEL AEHGDDEFID VCNSGLLLYQ ELHNQLYEYQ
110 120 130 140 150
KEGIAFLYSL YRDGRRGGIL ADDMGLGKTV QIIAFLSGMF DASLVNHVLL
160 170 180 190 200
IMPTSLISTW LREFVKWTPG MRVKTFHGPS KDERTRNLCR IQQRNGVIIT
210 220 230 240 250
TYQMLINNWQ QLSSLNGQEF LWDYVILDEA HKIKSSSTKS AICARAIPAS
260 270 280 290 300
NRILLTGTPI QNNLQELWSL FDFACQGSLL GTLRTFKMEY ENPITRAREK
310 320 330 340 350
DATPGEKALG FKISENLMAI IKPYFLRRTK EEVQKKKSSN PEVQLSEKSP
360 370 380 390 400
DVGAICEMPS LSRKNDLIIW IRLVPLQEEI YRKFVSLDHI KELLMETRSP
410 420 430 440 450
LAELGVLKKL CDHPRLLSAR ACGLLNLGAA KFSVQDEIEG EDSSDVDHID
460 470 480 490 500
QISDDTLMEE SGKMLFLMDL LKKLRDEGHQ TLVFSQSRRI LNIIERLLKN
510 520 530 540 550
RHFKILRIDG TITHLVEREK RISLFQQNKD YSVFLLTTQV GGVGLTLTAA
560 570 580 590 600
SRVVIFDPSW NPATDAQAVD RVYRIGQKEN VVVYRLITCG TVEEKIYRRQ
610 620 630 640 650
VFKDSLIRQT TGDKKNPFRY FSKQELRELF TIEDFQNSAT QLQLQSLHAA
660 670 680 690 700
QRRSDKNLDE HIAFLHSLRI AGISDHDLMY TRDLSVKEEL DVIEDSHYIQ
710 720 730 740 750
QRVQKAQFLV ESESQNTELL MERQKMGNEG IWLREPVYQT KKKRPKVNKP
760 770 780 790 800
QPQPSSHLPV YHTQEEISSL MASIIIDDLP KEDEKDLSRI KLNDTIPQDG
810 820 830 840 850
RHPCVITLND DFVTTLPKGC GDVEEIFPDS LSGMALQKEA SQEGFMQESE
860 870 880 890 900
QESPLASFNY LPSKSARVDL GPNLDQLEDD EVLHHCNPSP ANPKTKEYQR
910 920 930 940 950
PESNVSVIKI ADDDLTAAHS ALQDAQANEA KLEEEPLASS GQYACDFNLF
960 970 980 990 1000
LEDSADNGQN LSSQFLEHVE KENSLCGSAA NSRAESVHSK ACLSVDLSEE
1010 1020 1030 1040 1050
DDEPEEVVNV KIRRKARRID SDNEDEHTFK DTSSTNPFNT SPFPFLSVKQ
1060 1070 1080 1090 1100
FDASTPKNDI SLPERFFSPK ISDSINKSVN SRRSLASRRS LINVVLDHVE
1110 1120 1130 1140 1150
DMEERLDNSS EAKVVEDHLE EGAEESGDEA PEHTKEDPSR ETLSSENKSS
1160 1170 1180 1190 1200
QLSTSKPGAL AQETSPGDPE PLSDGQLVDS PQDEAMEAVN DYDTLVLHGK
1210 1220 1230 1240
ELKECGKIQE ALDCLVKALD IKSSDPEVML MTLSLYKQLN KT
Length:1,242
Mass (Da):140,510
Last modified:August 21, 2007 - v1
Checksum:iFF6F937D56CA8C6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC149963 mRNA. Translation: AAI49964.1.
BT026201 mRNA. Translation: ABG67040.1.
RefSeqiNP_001096000.1. NM_001102530.2.
UniGeneiBt.74729.

Genome annotation databases

EnsembliENSBTAT00000007362; ENSBTAP00000007362; ENSBTAG00000005607.
GeneIDi782916.
KEGGibta:782916.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC149963 mRNA. Translation: AAI49964.1.
BT026201 mRNA. Translation: ABG67040.1.
RefSeqiNP_001096000.1. NM_001102530.2.
UniGeneiBt.74729.

3D structure databases

ProteinModelPortaliA6QQR4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007362.

Proteomic databases

PaxDbiA6QQR4.
PRIDEiA6QQR4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000007362; ENSBTAP00000007362; ENSBTAG00000005607.
GeneIDi782916.
KEGGibta:782916.

Organism-specific databases

CTDi54821.

Phylogenomic databases

eggNOGiKOG0387. Eukaryota.
ENOG410XP4Z. LUCA.
GeneTreeiENSGT00590000083118.
HOGENOMiHOG000074172.
HOVERGENiHBG107854.
InParanoidiA6QQR4.
KOiK20093.
OMAiICEMPSL.
OrthoDBiEOG091G058Q.
TreeFamiTF332843.

Enzyme and pathway databases

ReactomeiR-BTA-2467813. Separation of Sister Chromatids.
R-BTA-2500257. Resolution of Sister Chromatid Cohesion.
R-BTA-5663220. RHO GTPases Activate Formins.
R-BTA-68877. Mitotic Prometaphase.

Gene expression databases

BgeeiENSBTAG00000005607.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERC6L_BOVIN
AccessioniPrimary (citable) accession number: A6QQR4
Secondary accession number(s): Q0V8L7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: August 21, 2007
Last modified: September 7, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.