A6QPY0 (OST48_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Short name=DDOST 48 kDa subunit
Short name=Oligosaccharyl transferase 48 kDa subunit
EC=2.4.1.119

Gene names

Name:

DDOST

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes even after release from the ribosome By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Belongs to the DDOST 48 kDa subunit family.
Sequence caution	The sequence ABG81441.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW oligosaccharyltransferase complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	By similarity
	Chain	27 – 439	413	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit		PRO_0000354671
Regions
	Topological domain	27 – 409	383	Lumenal Potential
	Transmembrane	410 – 430	21	Helical; Potential
	Topological domain	431 – 439	9	Cytoplasmic Potential
Experimental info
	Sequence conflict	114	1	S → G in AAI49547. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	A6QPY0 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: 9453193CC05CEDC5	FASTA	439	48,791
	10 20 30 40 50 60 MATRAARVWS GWWLLLLPLL GLAGASGPRT LVLLDNLNLR ETHSLFFRSL KDRGFVLTFK 70 80 90 100 110 120 TADDPSLSLI KYGEFLYDNL IIFSPSVEDF GGNINVETIS AFIDGGGSVL VAASSDIGDP 130 140 150 160 170 180 LRELGSECGI EFDEEKTAVI DHHNYDVSDL GQHTLIVADT ENLLKAPTIV GKSSLNPILF 190 200 210 220 230 240 RGVGMVADPD NPLVLDILTG SSTSYSFFPD KPITQYPHAV GKNTLLIAGL QARNNARVIF 250 260 270 280 290 300 SGSLDFFSDA FFNSAVQKAA PGSQRYSQTG NYELAVALSR WVFKEEGVLR VGPVSHHRVG 310 320 330 340 350 360 ETAPPNAYTV TDLVEYSIVI EQLSDGKWVP FDGDDIQLEF VRIDPFVRTF LKRKGGKYSV 370 380 390 400 410 420 QFKLPDVYGV FQFKVDYNRL GYTHLYSSTQ VSVRPLQHTQ YERFIPSAYP YYASAFSMML 430 GLFIFSVVFL HMKEKEKSD

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-393.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC149546 mRNA. Translation: AAI49547.1. BT026285 mRNA. Translation: ABG81441.1. Different initiation.
IPI	IPI00867276.
RefSeq	NP_001094543.1. NM_001101073.1.
UniGene	Bt.23351.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6QPY0.
Proteomic databases
PRIDE	A6QPY0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	510682.
KEGG	bta:510682.
Organism-specific databases
CTD	1650.
Phylogenomic databases
GeneTree	ENSGT00390000017294.
HOVERGEN	HBG053378.
InParanoid	A6QPY0.
OrthoDB	EOG45HRXC.
Family and domain databases
InterPro	IPR005013. OligosaccharylTrfase_su_Wbp1. [Graphical view]
KO	K12670.
PANTHER	PTHR10830. DDOST_48kDa. 1 hit.
Pfam	PF03345. DDOST_48kD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OST48_BOVIN

Accession

Primary (citable) accession number: A6QPY0
Secondary accession number(s): Q0V8D4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	December 14, 2011
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program