A6QPU5 (SYDM_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase, mitochondrial
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:

DARS2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 44	44	Mitochondrion Potential
	Chain	45 – 651	607	Aspartate--tRNA ligase, mitochondrial		PRO_0000327860
Amino acid modifications
	Modified residue	232	1	N6-acetyllysine By similarity
	Modified residue	379	1	N6-acetyllysine By similarity
	Modified residue	624	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6QPU5 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: BF9790C9E0C877C7	FASTA	651	73,688
	10 20 30 40 50 60 MFCWLSRLCG ELSTPTRRTT QLIWSSAARS MVLSSQRIPE LSSFVARTNT CGELRSSHLG 70 80 90 100 110 120 QEVTLCGWIQ FRRQNIFLVL RDFHGLVQVV IPQDESAASV KKILCEAPME SVVQVSGTVI 130 140 150 160 170 180 SRPPGQKNPK MPTGEIEIKV KTAKLLNSCK KLPFEIKDFM KKTETLRLQY RYLDLRSVQM 190 200 210 220 230 240 QYNLRLRSQM VMKMREYLCN LHGFVDVETP TLFKRTPGGA KEFVIPSREP GKFYSLPQSP 250 260 270 280 290 300 QQFKQLLMVG GLDRYFQVAR CYRDEGSRPD RQPEFTQIDI EMSFVDQTGV QSLIEGLLQY 310 320 330 340 350 360 SWPSDKDPLV VPFPSMPFAE ALASYGTDKP DTRFGMKIVD ISDMFRNTEV GFLQDALSKP 370 380 390 400 410 420 QGTVKAICIR KGAKYLKRKD IESIRKFAAD HFNEEVLPIF LKTNENWNSP VAKFIMEEQG 430 440 450 460 470 480 LGLVKLLETQ EEDVVLLTAG EHKKACSLMG KLRLECADLL EARGVVLRDP ALFSFLWVVD 490 500 510 520 530 540 FPLFLPKEEN PQELESAHHP FTAPHPSDIH LLYTEPHKVR SQHYDLVLNG NEIGGGSIRI 550 560 570 580 590 600 HNSELQHCVL DTVLKEDVKL LSHLLQALDY GAPPHGGIAL GLDRLMCLVT GAPSIRDVIA 610 620 630 640 650 FPKSFRGHDL MSNAPDSIPP EELKPYHIQV SWPMDAETEK SSSNHPCRSE S

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Thymus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC149492 mRNA. Translation: AAI49493.1.
IPI	IPI00867017.
RefSeq	NP_001095692.1. NM_001102222.1.
UniGene	Bt.103622.
3D structure databases
ProteinModelPortal	A6QPU5.
ModBase	Search...
Protein-protein interaction databases
STRING	A6QPU5.
Proteomic databases
PRIDE	A6QPU5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	538772.
KEGG	bta:538772.
Organism-specific databases
CTD	55157.
Phylogenomic databases
eggNOG	maNOG04388.
GeneTree	ENSGT00550000074971.
HOVERGEN	HBG055815.
InParanoid	A6QPU5.
OrthoDB	EOG4M91R5.
Family and domain databases
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYDM_BOVIN

Accession

Primary (citable) accession number: A6QPU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	August 21, 2007
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program