Reviewed, UniProtKB/Swiss-Prot A6QPM3 (PRDM6_BOVIN)

Names and origin

Protein names

Recommended name:
    Putative histone-lysine N-methyltransferase PRDM6
    EC=2.1.1.43
Alternative name(s):
    PR domain zinc finger protein 6
    PR domain-containing protein 6

Gene names

Name:

PRDM6

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Putative histone methyltransferase that acts as a transcriptional repressor of smooth muscle gene expression. Promotes the transition from differentiated to proliferative smooth muscle by suppressing differentiation and maintaining the proliferative potential of vascular smooth muscle cells. Also plays a role in endothelial cells by inhibiting endothelial cell proliferation, survival and differentiation. It is unclear whether it has histone methyltransferase activity in vivo. According to some authors, it does not act as a histone methyltransferase by itself and represses transcription by recruiting EHMT2/G9a. According to others, it possesses histone methyltransferase activity when associated with other proteins and specifically methylates 'Lys-20' of histone H4 in vitro. 'Lys-20' methylation represents a specific tag for epigenetic transcriptional repression By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
Subunit structure	Interacts with HDAC1, HDAC2, HDAC3, CBX1 and EP300 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Contains 4 C2H2-type zinc fingers. Contains 1 SET domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Chromosomal protein Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding S-adenosyl-L-methionine Zinc
Molecular function	Chromatin regulator Methyltransferase Repressor Transferase
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Putative histone-lysine N-methyltransferase PRDM6		PRO_0000363958
Regions
	Domain	242 – 364	123	SET
	Zinc finger	468 – 490	23	C2H2-type 1; degenerate
	Zinc finger	496 – 518	23	C2H2-type 2
	Zinc finger	524 – 546	23	C2H2-type 3
	Zinc finger	552 – 574	23	C2H2-type 4; degenerate
	Compositional bias	30 – 33	4	Poly-Gly
	Compositional bias	43 – 70	28	Pro-rich
	Compositional bias	94 – 106	13	Poly-Ala
	Compositional bias	223 – 227	5	Poly-Ala

Sequences

	Sequence		Length	Mass (Da)
	A6QPM3-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: B7D55FF617059231	FASTA	590	63,989
	10 20 30 40 50 60 MLKPGDPGGS AFLKVDPAYL QHWQQLFPHG GGGPLKGGGA AGPLGAPQPL QPPPPPERAE 70 80 90 100 110 120 PQPDSLRPRP ASLSSASSTP ASSSTSASSA SSCAAAAAAA AAAALAGLSA LPVAQLPVFA 130 140 150 160 170 180 PLATVAAEPL PPKDLCLGAT SGPGPSKCGG SGGDGRGVPR FRCSAEELDY YLYGQQRMEI 190 200 210 220 230 240 IPLNQHTSDP NNRCDMCADN RNGECPMHGP LHSLRRLVGT SSAAAAAPPP ELPEWLRDLP 250 260 270 280 290 300 REVCLCTSTV PGLAYGICAA QRIQQGTWIG PFQGVLLPPE KVQAGAVRNT QHLWEIYDQD 310 320 330 340 350 360 GTLQHFIDGG EPSKSSWMRY IRCARHCGEQ NLTVVQYRSN IFYRACIDIP RGTELLVWYN 370 380 390 400 410 420 DSYTSFFGIP LQCIAQDENL NVPSTVMEAM CRQDALQPFN KSSKLSQAPQ QRSVVFPQTP 430 440 450 460 470 480 CGRNFSLLDK SGPLESGFNQ ISVKNQRVLA SPTSTSQLHS EFSDWHLWKC GQCFKTFTQR 490 500 510 520 530 540 ILLQMHVCTQ NPDRPYQCGH CSQSFSQPSE LRNHVVTHSS DRPFKCGYCG RAFAGATTLN 550 560 570 580 590 NHIRTHTGEK PFKCERCERS FTQATQLSRH QRMPNECKPI TESPESIEVD

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal spinal cord.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC149387 mRNA. Translation: AAI49388.1.
IPI	IPI00715269.
RefSeq	NP_001096725.1.
UniGene	Bt.36375
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000027421; ENSBTAP00000027421; ENSBTAG00000020578; Bos taurus. [Genome view]
GeneID	519857.
KEGG	bta:519857.
Organism-specific databases
CTD	519857.
Phylogenomic databases
OMA	LQPFSKS.
Family and domain databases
InterPro	IPR001214. SET. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 2 hits.
Pfam	PF00856. SET. 1 hit. PF00096. zf-C2H2. 3 hits. [Graphical view]
SMART	SM00317. SET. 1 hit. SM00355. ZnF_C2H2. 4 hits. [Graphical view]
PROSITE	PS50280. SET. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 2 hits. PS50157. ZINC_FINGER_C2H2_2. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRDM6_BOVIN

Accession

Primary (citable) accession number: A6QPM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	August 21, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)