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Protein

Putative histone-lysine N-methyltransferase PRDM6

Gene

PRDM6

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Putative histone methyltransferase that acts as a transcriptional repressor of smooth muscle gene expression. Promotes the transition from differentiated to proliferative smooth muscle by suppressing differentiation and maintaining the proliferative potential of vascular smooth muscle cells. Also plays a role in endothelial cells by inhibiting endothelial cell proliferation, survival and differentiation. It is unclear whether it has histone methyltransferase activity in vivo. According to some authors, it does not act as a histone methyltransferase by itself and represses transcription by recruiting EHMT2/G9a. According to others, it possesses histone methyltransferase activity when associated with other proteins and specifically methylates 'Lys-20' of histone H4 in vitro. 'Lys-20' methylation represents a specific tag for epigenetic transcriptional repression (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri468 – 49023C2H2-type 1; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri496 – 51823C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri524 – 54623C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri552 – 57423C2H2-type 4; degeneratePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Repressor, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, S-adenosyl-L-methionine, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Putative histone-lysine N-methyltransferase PRDM6 (EC:2.1.1.43)
Alternative name(s):
PR domain zinc finger protein 6
PR domain-containing protein 6
Gene namesi
Name:PRDM6
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 590590Putative histone-lysine N-methyltransferase PRDM6PRO_0000363958Add
BLAST

Proteomic databases

PaxDbiA6QPM3.

Expressioni

Gene expression databases

BgeeiENSBTAG00000020578.

Interactioni

Subunit structurei

Interacts with HDAC1, HDAC2, HDAC3, CBX1 and EP300.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027421.

Structurei

3D structure databases

ProteinModelPortaliA6QPM3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini241 – 360120SETPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi30 – 334Poly-Gly
Compositional biasi43 – 7028Pro-richAdd
BLAST
Compositional biasi94 – 10613Poly-AlaAdd
BLAST
Compositional biasi223 – 2275Poly-Ala

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily.PROSITE-ProRule annotation
Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 SET domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri468 – 49023C2H2-type 1; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri496 – 51823C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri524 – 54623C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri552 – 57423C2H2-type 4; degeneratePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
KOG2461. Eukaryota.
ENOG410XRVC. LUCA.
GeneTreeiENSGT00830000128284.
HOGENOMiHOG000090218.
HOVERGENiHBG108289.
InParanoidiA6QPM3.
OMAiPPEIPEW.
OrthoDBiEOG091G0A5R.
TreeFamiTF106403.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR001214. SET_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00317. SET. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS50280. SET. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QPM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKPGDPGGS AFLKVDPAYL QHWQQLFPHG GGGPLKGGGA AGPLGAPQPL
60 70 80 90 100
QPPPPPERAE PQPDSLRPRP ASLSSASSTP ASSSTSASSA SSCAAAAAAA
110 120 130 140 150
AAAALAGLSA LPVAQLPVFA PLATVAAEPL PPKDLCLGAT SGPGPSKCGG
160 170 180 190 200
SGGDGRGVPR FRCSAEELDY YLYGQQRMEI IPLNQHTSDP NNRCDMCADN
210 220 230 240 250
RNGECPMHGP LHSLRRLVGT SSAAAAAPPP ELPEWLRDLP REVCLCTSTV
260 270 280 290 300
PGLAYGICAA QRIQQGTWIG PFQGVLLPPE KVQAGAVRNT QHLWEIYDQD
310 320 330 340 350
GTLQHFIDGG EPSKSSWMRY IRCARHCGEQ NLTVVQYRSN IFYRACIDIP
360 370 380 390 400
RGTELLVWYN DSYTSFFGIP LQCIAQDENL NVPSTVMEAM CRQDALQPFN
410 420 430 440 450
KSSKLSQAPQ QRSVVFPQTP CGRNFSLLDK SGPLESGFNQ ISVKNQRVLA
460 470 480 490 500
SPTSTSQLHS EFSDWHLWKC GQCFKTFTQR ILLQMHVCTQ NPDRPYQCGH
510 520 530 540 550
CSQSFSQPSE LRNHVVTHSS DRPFKCGYCG RAFAGATTLN NHIRTHTGEK
560 570 580 590
PFKCERCERS FTQATQLSRH QRMPNECKPI TESPESIEVD
Length:590
Mass (Da):63,989
Last modified:August 21, 2007 - v1
Checksum:iB7D55FF617059231
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC149387 mRNA. Translation: AAI49388.1.
RefSeqiNP_001096725.1. NM_001103255.2.
UniGeneiBt.36375.

Genome annotation databases

EnsembliENSBTAT00000027421; ENSBTAP00000027421; ENSBTAG00000020578.
GeneIDi519857.
KEGGibta:519857.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC149387 mRNA. Translation: AAI49388.1.
RefSeqiNP_001096725.1. NM_001103255.2.
UniGeneiBt.36375.

3D structure databases

ProteinModelPortaliA6QPM3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027421.

Proteomic databases

PaxDbiA6QPM3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000027421; ENSBTAP00000027421; ENSBTAG00000020578.
GeneIDi519857.
KEGGibta:519857.

Organism-specific databases

CTDi93166.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
KOG2461. Eukaryota.
ENOG410XRVC. LUCA.
GeneTreeiENSGT00830000128284.
HOGENOMiHOG000090218.
HOVERGENiHBG108289.
InParanoidiA6QPM3.
OMAiPPEIPEW.
OrthoDBiEOG091G0A5R.
TreeFamiTF106403.

Gene expression databases

BgeeiENSBTAG00000020578.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR001214. SET_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00317. SET. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS50280. SET. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRDM6_BOVIN
AccessioniPrimary (citable) accession number: A6QPM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: August 21, 2007
Last modified: September 7, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.