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Protein

Protein-glutamine gamma-glutamyltransferase E

Gene

TGM3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold. In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath (By similarity).By similarity

Catalytic activityi

Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ cations per subunit. Binds 1 Ca2+ as a zymogen, and binds 2 more Ca2+ cations, or other divalent metal cations, after proteolytic processing.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi221Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi224Calcium 1By similarity1
Metal bindingi226Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi227Calcium 1By similarity1
Active sitei272PROSITE-ProRule annotation1
Metal bindingi301Calcium 2By similarity1
Metal bindingi303Calcium 2By similarity1
Metal bindingi305Calcium 2By similarity1
Metal bindingi307Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi324Calcium 2By similarity1
Active sitei330PROSITE-ProRule annotation1
Active sitei353PROSITE-ProRule annotation1
Metal bindingi393Calcium 3By similarity1
Metal bindingi414Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi442Calcium 3By similarity1
Metal bindingi447Calcium 3By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Keratinization

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-glutamine gamma-glutamyltransferase E (EC:2.3.2.13)
Alternative name(s):
Transglutaminase E
Short name:
TG(E)
Short name:
TGE
Short name:
TGase E
Transglutaminase-3
Short name:
TGase-3
Cleaved into the following 2 chains:
Gene namesi
Name:TGM3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004089491 – 465Protein-glutamine gamma-glutamyltransferase E 50 kDa catalytic chainAdd BLAST465
ChainiPRO_0000408950466 – 691Protein-glutamine gamma-glutamyltransferase E 27 kDa non-catalytic chainAdd BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei110PhosphotyrosineBy similarity1
Modified residuei111PhosphothreonineBy similarity1

Post-translational modificationi

Activated by proteolytic processing. In vitro activation is commonly achieved by cleavage with dispase, a neutral bacterial protease. Physiological activation may be catalyzed by CTSL and, to a lesser extent, by CTSS (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei465 – 466Cleavage; by CTSL2

Keywords - PTMi

Phosphoprotein, Zymogen

Proteomic databases

PaxDbiA6QP57.
PeptideAtlasiA6QP57.
PRIDEiA6QP57.

Interactioni

Subunit structurei

Consists of two polypeptide chains, which are synthesized as a precursor form of a single polypeptide.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006432.

Structurei

3D structure databases

ProteinModelPortaliA6QP57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFMV. Eukaryota.
ENOG410XQEZ. LUCA.
HOGENOMiHOG000231695.
HOVERGENiHBG004342.
InParanoidiA6QP57.
KOiK05620.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR013808. Transglutaminase_AS.
IPR008958. Transglutaminase_C.
IPR001102. Transglutaminase_N.
[Graphical view]
PANTHERiPTHR11590. PTHR11590. 1 hit.
PfamiPF00927. Transglut_C. 2 hits.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
SMARTiSM00460. TGc. 1 hit.
[Graphical view]
SUPFAMiSSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6QP57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLQSVDWQ IASNRQAHHT ERFYGKDLLV RRGQLFQVSL TLSQGLSSGG
60 70 80 90 100
RVTFTASTGP YPSESANTKA VFPLSNGTSS SGWGAQLVSS RNNVLNISIL
110 120 130 140 150
SPANAPIGRY TLNMQISSQG SDSTLKLGTF ILLFNPWLQA DSVFMSNHAE
160 170 180 190 200
REEYVQEDAG IIFVGSTNRI SMIGWNYGQF EEGILNICLS VLDNSLNFRR
210 220 230 240 250
DPATDVAHRN DPKYVGRVLS AMINGNDDSG VISGNWSGSY TGGRDPRNWN
260 270 280 290 300
GSVEILKEWQ RSGFRPVRYG QCWVFAGTLN TVLRCLGIPS RVITNFNSAH
310 320 330 340 350
DTDQNLSVDV YYDPLGRPMD KGSDSVWNFH VWNEAWFVRS DLGPSYNGWQ
360 370 380 390 400
VLDATPQERS QGVFQCGPAS VIAIREGNVD WDFDMPFIFA EVNADRITWI
410 420 430 440 450
YESNGALKKN SADTHSVGKH ISTKAVGSNS RMDVTEKYKY PEGSSQERQV
460 470 480 490 500
FEKALRKLKP TMSFSATSAS SLAREEREPS ISGRFKVAGV LTVGKEVNLI
510 520 530 540 550
LMLKNLTSDT KTVTVNMTAW TIVYNGTLVH EVWKDSVTKS LNPEEEIEHP
560 570 580 590 600
VKIAYAQYEK YLKADNMIRT TAVCQVTDEP EVVVERDIIL DNPTLTLEVL
610 620 630 640 650
DEARVQKPVN VQMLFSNPLD EPVKDCVLMV EGSGLLLGNL KIDVPALRPK
660 670 680 690
ERSRVRFEIL PTRSGTKQLL ANFSCNKFPA IKAMLSVDVA E
Length:691
Mass (Da):76,791
Last modified:August 21, 2007 - v1
Checksum:i67DB0852E0973B4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC149161 mRNA. Translation: AAI49162.1.
RefSeqiNP_001095318.1. NM_001101848.1.
UniGeneiBt.13628.

Genome annotation databases

GeneIDi505080.
KEGGibta:505080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC149161 mRNA. Translation: AAI49162.1.
RefSeqiNP_001095318.1. NM_001101848.1.
UniGeneiBt.13628.

3D structure databases

ProteinModelPortaliA6QP57.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006432.

Proteomic databases

PaxDbiA6QP57.
PeptideAtlasiA6QP57.
PRIDEiA6QP57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi505080.
KEGGibta:505080.

Organism-specific databases

CTDi7053.

Phylogenomic databases

eggNOGiENOG410IFMV. Eukaryota.
ENOG410XQEZ. LUCA.
HOGENOMiHOG000231695.
HOVERGENiHBG004342.
InParanoidiA6QP57.
KOiK05620.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR013808. Transglutaminase_AS.
IPR008958. Transglutaminase_C.
IPR001102. Transglutaminase_N.
[Graphical view]
PANTHERiPTHR11590. PTHR11590. 1 hit.
PfamiPF00927. Transglut_C. 2 hits.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
SMARTiSM00460. TGc. 1 hit.
[Graphical view]
SUPFAMiSSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTGM3_BOVIN
AccessioniPrimary (citable) accession number: A6QP57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: August 21, 2007
Last modified: October 5, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.