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A6QP16

- ZRAN1_BOVIN

UniProt

A6QP16 - ZRAN1_BOVIN

Protein

Ubiquitin thioesterase ZRANB1

Gene

ZRANB1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. May also modulate TNF-alpha signaling By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei443 – 4431NucleophileBy similarity
    Active sitei585 – 5851Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri3 – 3331RanBP2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri84 – 11330RanBP2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri149 – 17830RanBP2-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: UniProtKB
    2. ubiquitin thiolesterase activity Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cell migration Source: UniProtKB
    2. cytoskeleton organization Source: UniProtKB
    3. positive regulation of Wnt signaling pathway Source: UniProtKB
    4. protein deubiquitination involved in ubiquitin-dependent protein catabolic process Source: Ensembl
    5. protein K29-linked deubiquitination Source: UniProtKB
    6. protein K33-linked deubiquitination Source: UniProtKB
    7. protein K63-linked deubiquitination Source: UniProtKB
    8. regulation of cell morphogenesis Source: UniProtKB
    9. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway, Wnt signaling pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC64.004.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin thioesterase ZRANB1 (EC:3.4.19.12)
    Alternative name(s):
    Zinc finger Ran-binding domain-containing protein 1
    Gene namesi
    Name:ZRANB1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 26

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: Enriched in punctate localization in the cytoplasm.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 708708Ubiquitin thioesterase ZRANB1PRO_0000361553Add
    BLAST

    Proteomic databases

    PRIDEiA6QP16.

    Interactioni

    Subunit structurei

    Interacts with APC and TRAF6.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000004401.

    Structurei

    3D structure databases

    ProteinModelPortaliA6QP16.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati260 – 29031ANK 1Add
    BLAST
    Repeati313 – 34028ANK 2Add
    BLAST
    Domaini432 – 592161OTUPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The OTU domain mediates the deubiquitinating activity.By similarity
    The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin.By similarity
    The second ankyrin repeat ANK 2 is termed AnkUBD, it interacts with ubiquitin hydrophobic patch and contributes to linkage specificity.By similarity
    The RanBP2-type zinc fingers, also called NZFs, may provide additional ubiquitin-binding sites when hydrolyzing long 'Lys-63'-linked chains.By similarity

    Sequence similaritiesi

    Belongs to the peptidase C64 family.Curated
    Contains 2 ANK repeats.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation
    Contains 3 RanBP2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri3 – 3331RanBP2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri84 – 11330RanBP2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri149 – 17830RanBP2-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    ANK repeat, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG253754.
    GeneTreeiENSGT00530000062989.
    HOGENOMiHOG000006743.
    HOVERGENiHBG058978.
    InParanoidiA6QP16.
    KOiK11862.
    OMAiWTCSICT.
    OrthoDBiEOG7R56SB.
    TreeFamiTF323312.

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR001876. Znf_RanBP2.
    [Graphical view]
    PfamiPF02338. OTU. 1 hit.
    PF00641. zf-RanBP. 2 hits.
    [Graphical view]
    SMARTiSM00547. ZnF_RBZ. 3 hits.
    [Graphical view]
    PROSITEiPS50802. OTU. 1 hit.
    PS01358. ZF_RANBP2_1. 3 hits.
    PS50199. ZF_RANBP2_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6QP16-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSERGIKWAC EYCTYENWPS AIKCTMCRAQ RPSGTIITED PFKSGSSDVG    50
    RDWDPSSTEG GSSPLICPDS SARPRVKSSY SMENANKWSC HMCTYLNWPR 100
    AIRCTQCLSQ RRTRSPTESP QSSGSGSRPV AFSVDPCEEY NDRNKLNTRT 150
    QHWTCSICTY ENWAKAKKCV VCDHPRPNNI EAIEFAETEE ASSIINEQDR 200
    ARWRGSCSSG NSQRRSPPTM KRDSEVKMDF QRIELAGAVG SKEELEVDFK 250
    KLKQIKNRMK KTDWLFLNAC VGVVEGDLAA IEAYKSSGGD IARQLTADEV 300
    RLLNRPSAFD VGYTLVHLAI RFQRQDMLAI LLTEVSQQAA KCIPAMVCPE 350
    LTEQIRREIA ASLHQRKGDF ACYFLTDLVT FTLPADIEDL PPTVQEKLFD 400
    EVLDRDVQKE LEEESPIINW SLELATRLDS RLYALWNRTA GDCLLDSVLQ 450
    ATWGIYDKDS VLRKALHDSL HDCSHWFYTR WKDWESWYSQ SFGLHFSLRE 500
    EQWQEDWAFI LSLASQPGAS LEQTHIFVLA HILRRPIIVY GVKYYKSFRG 550
    ETLGYTRFQG VYLPLLWEQS FCWKSPIALG YTRGHFSALV AMENDGYGNR 600
    GAGANLNTDD DVTITFLPLV DSERKLLHVH FLSAQELGNE EQQEKLLREW 650
    LDCCVTEGGV LVAMQKSSRR RNHPLVTQMV EKWLDRYRQI RPCTSLSDGE 700
    EDEDDEDE 708
    Length:708
    Mass (Da):81,047
    Last modified:August 21, 2007 - v1
    Checksum:i386832570C7AEF03
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC149099 mRNA. Translation: AAI49100.1.
    RefSeqiNP_001094584.1. NM_001101114.1.
    UniGeneiBt.53702.

    Genome annotation databases

    EnsembliENSBTAT00000004401; ENSBTAP00000004401; ENSBTAG00000003395.
    GeneIDi523338.
    KEGGibta:523338.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC149099 mRNA. Translation: AAI49100.1 .
    RefSeqi NP_001094584.1. NM_001101114.1.
    UniGenei Bt.53702.

    3D structure databases

    ProteinModelPortali A6QP16.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000004401.

    Protein family/group databases

    MEROPSi C64.004.

    Proteomic databases

    PRIDEi A6QP16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000004401 ; ENSBTAP00000004401 ; ENSBTAG00000003395 .
    GeneIDi 523338.
    KEGGi bta:523338.

    Organism-specific databases

    CTDi 54764.

    Phylogenomic databases

    eggNOGi NOG253754.
    GeneTreei ENSGT00530000062989.
    HOGENOMi HOG000006743.
    HOVERGENi HBG058978.
    InParanoidi A6QP16.
    KOi K11862.
    OMAi WTCSICT.
    OrthoDBi EOG7R56SB.
    TreeFami TF323312.

    Miscellaneous databases

    NextBioi 20873719.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR001876. Znf_RanBP2.
    [Graphical view ]
    Pfami PF02338. OTU. 1 hit.
    PF00641. zf-RanBP. 2 hits.
    [Graphical view ]
    SMARTi SM00547. ZnF_RBZ. 3 hits.
    [Graphical view ]
    PROSITEi PS50802. OTU. 1 hit.
    PS01358. ZF_RANBP2_1. 3 hits.
    PS50199. ZF_RANBP2_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal skin.

    Entry informationi

    Entry nameiZRAN1_BOVIN
    AccessioniPrimary (citable) accession number: A6QP16
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3