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A6QP16

- ZRAN1_BOVIN

UniProt

A6QP16 - ZRAN1_BOVIN

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Protein
Ubiquitin thioesterase ZRANB1
Gene
ZRANB1
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. May also modulate TNF-alpha signaling By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei443 – 4431Nucleophile By similarity
Active sitei585 – 5851Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 3331RanBP2-type 1
Add
BLAST
Zinc fingeri84 – 11330RanBP2-type 2
Add
BLAST
Zinc fingeri149 – 17830RanBP2-type 3
Add
BLAST

GO - Molecular functioni

  1. ubiquitin thiolesterase activity Source: UniProtKB
  2. ubiquitin-specific protease activity Source: UniProtKB
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. Wnt signaling pathway Source: UniProtKB-KW
  2. cell migration Source: UniProtKB
  3. cytoskeleton organization Source: UniProtKB
  4. positive regulation of Wnt signaling pathway Source: UniProtKB
  5. protein K29-linked deubiquitination Source: UniProtKB
  6. protein K33-linked deubiquitination Source: UniProtKB
  7. protein K63-linked deubiquitination Source: UniProtKB
  8. protein deubiquitination involved in ubiquitin-dependent protein catabolic process Source: Ensembl
  9. regulation of cell morphogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway, Wnt signaling pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC64.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase ZRANB1 (EC:3.4.19.12)
Alternative name(s):
Zinc finger Ran-binding domain-containing protein 1
Gene namesi
Name:ZRANB1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 26

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Enriched in punctate localization in the cytoplasm By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 708708Ubiquitin thioesterase ZRANB1
PRO_0000361553Add
BLAST

Proteomic databases

PRIDEiA6QP16.

Interactioni

Subunit structurei

Interacts with APC and TRAF6 By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004401.

Structurei

3D structure databases

ProteinModelPortaliA6QP16.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati260 – 29031ANK 1
Add
BLAST
Repeati313 – 34028ANK 2
Add
BLAST
Domaini432 – 592161OTU
Add
BLAST

Domaini

The OTU domain mediates the deubiquitinating activity By similarity.
The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin By similarity.
The second ankyrin repeat ANK 2 is termed AnkUBD, it interacts with ubiquitin hydrophobic patch and contributes to linkage specificity By similarity.
The RanBP2-type zinc fingers, also called NZFs, may provide additional ubiquitin-binding sites when hydrolyzing long 'Lys-63'-linked chains By similarity.

Sequence similaritiesi

Belongs to the peptidase C64 family.
Contains 2 ANK repeats.
Contains 1 OTU domain.

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG253754.
GeneTreeiENSGT00530000062989.
HOGENOMiHOG000006743.
HOVERGENiHBG058978.
InParanoidiA6QP16.
KOiK11862.
OMAiWTCSICT.
OrthoDBiEOG7R56SB.
TreeFamiTF323312.

Family and domain databases

InterProiIPR003323. OTU.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
PF00641. zf-RanBP. 2 hits.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 3 hits.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS01358. ZF_RANBP2_1. 3 hits.
PS50199. ZF_RANBP2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QP16-1 [UniParc]FASTAAdd to Basket

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MSERGIKWAC EYCTYENWPS AIKCTMCRAQ RPSGTIITED PFKSGSSDVG    50
RDWDPSSTEG GSSPLICPDS SARPRVKSSY SMENANKWSC HMCTYLNWPR 100
AIRCTQCLSQ RRTRSPTESP QSSGSGSRPV AFSVDPCEEY NDRNKLNTRT 150
QHWTCSICTY ENWAKAKKCV VCDHPRPNNI EAIEFAETEE ASSIINEQDR 200
ARWRGSCSSG NSQRRSPPTM KRDSEVKMDF QRIELAGAVG SKEELEVDFK 250
KLKQIKNRMK KTDWLFLNAC VGVVEGDLAA IEAYKSSGGD IARQLTADEV 300
RLLNRPSAFD VGYTLVHLAI RFQRQDMLAI LLTEVSQQAA KCIPAMVCPE 350
LTEQIRREIA ASLHQRKGDF ACYFLTDLVT FTLPADIEDL PPTVQEKLFD 400
EVLDRDVQKE LEEESPIINW SLELATRLDS RLYALWNRTA GDCLLDSVLQ 450
ATWGIYDKDS VLRKALHDSL HDCSHWFYTR WKDWESWYSQ SFGLHFSLRE 500
EQWQEDWAFI LSLASQPGAS LEQTHIFVLA HILRRPIIVY GVKYYKSFRG 550
ETLGYTRFQG VYLPLLWEQS FCWKSPIALG YTRGHFSALV AMENDGYGNR 600
GAGANLNTDD DVTITFLPLV DSERKLLHVH FLSAQELGNE EQQEKLLREW 650
LDCCVTEGGV LVAMQKSSRR RNHPLVTQMV EKWLDRYRQI RPCTSLSDGE 700
EDEDDEDE 708
Length:708
Mass (Da):81,047
Last modified:August 21, 2007 - v1
Checksum:i386832570C7AEF03
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC149099 mRNA. Translation: AAI49100.1.
RefSeqiNP_001094584.1. NM_001101114.1.
UniGeneiBt.53702.

Genome annotation databases

EnsembliENSBTAT00000004401; ENSBTAP00000004401; ENSBTAG00000003395.
GeneIDi523338.
KEGGibta:523338.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC149099 mRNA. Translation: AAI49100.1 .
RefSeqi NP_001094584.1. NM_001101114.1.
UniGenei Bt.53702.

3D structure databases

ProteinModelPortali A6QP16.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000004401.

Protein family/group databases

MEROPSi C64.004.

Proteomic databases

PRIDEi A6QP16.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000004401 ; ENSBTAP00000004401 ; ENSBTAG00000003395 .
GeneIDi 523338.
KEGGi bta:523338.

Organism-specific databases

CTDi 54764.

Phylogenomic databases

eggNOGi NOG253754.
GeneTreei ENSGT00530000062989.
HOGENOMi HOG000006743.
HOVERGENi HBG058978.
InParanoidi A6QP16.
KOi K11862.
OMAi WTCSICT.
OrthoDBi EOG7R56SB.
TreeFami TF323312.

Miscellaneous databases

NextBioi 20873719.

Family and domain databases

InterProi IPR003323. OTU.
IPR001876. Znf_RanBP2.
[Graphical view ]
Pfami PF02338. OTU. 1 hit.
PF00641. zf-RanBP. 2 hits.
[Graphical view ]
SMARTi SM00547. ZnF_RBZ. 3 hits.
[Graphical view ]
PROSITEi PS50802. OTU. 1 hit.
PS01358. ZF_RANBP2_1. 3 hits.
PS50199. ZF_RANBP2_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal skin.

Entry informationi

Entry nameiZRAN1_BOVIN
AccessioniPrimary (citable) accession number: A6QP16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: August 21, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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