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Protein

Ubiquitin thioesterase ZRANB1

Gene

ZRANB1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. May also modulate TNF-alpha signaling (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei443 – 4431NucleophileBy similarity
Active sitei585 – 5851Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 3331RanBP2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri84 – 11330RanBP2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri149 – 17830RanBP2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway, Wnt signaling pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC64.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase ZRANB1 (EC:3.4.19.12)
Alternative name(s):
Zinc finger Ran-binding domain-containing protein 1
Gene namesi
Name:ZRANB1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 26

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Enriched in punctate localization in the cytoplasm.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 708708Ubiquitin thioesterase ZRANB1PRO_0000361553Add
BLAST

Proteomic databases

PRIDEiA6QP16.

Interactioni

Subunit structurei

Interacts with APC and TRAF6.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004401.

Structurei

Secondary structure

1
708
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 133Combined sources
Beta strandi15 – 173Combined sources
Beta strandi25 – 273Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4S1ZX-ray3.03F/G/H/I/J2-33[»]
ProteinModelPortaliA6QP16.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati260 – 29031ANK 1Add
BLAST
Repeati313 – 34028ANK 2Add
BLAST
Domaini432 – 592161OTUPROSITE-ProRule annotationAdd
BLAST

Domaini

The OTU domain mediates the deubiquitinating activity.By similarity
The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin.By similarity
The second ankyrin repeat ANK 2 is termed AnkUBD, it interacts with ubiquitin hydrophobic patch and contributes to linkage specificity.By similarity
The RanBP2-type zinc fingers, also called NZFs, may provide additional ubiquitin-binding sites when hydrolyzing long 'Lys-63'-linked chains.By similarity

Sequence similaritiesi

Belongs to the peptidase C64 family.Curated
Contains 2 ANK repeats.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation
Contains 3 RanBP2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 3331RanBP2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri84 – 11330RanBP2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri149 – 17830RanBP2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG253754.
GeneTreeiENSGT00530000062989.
HOGENOMiHOG000006743.
HOVERGENiHBG058978.
InParanoidiA6QP16.
KOiK11862.
OMAiSEMKMDF.
OrthoDBiEOG7R56SB.
TreeFamiTF323312.

Family and domain databases

InterProiIPR003323. OTU_dom.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
PF00641. zf-RanBP. 2 hits.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 3 hits.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS01358. ZF_RANBP2_1. 3 hits.
PS50199. ZF_RANBP2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QP16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSERGIKWAC EYCTYENWPS AIKCTMCRAQ RPSGTIITED PFKSGSSDVG
60 70 80 90 100
RDWDPSSTEG GSSPLICPDS SARPRVKSSY SMENANKWSC HMCTYLNWPR
110 120 130 140 150
AIRCTQCLSQ RRTRSPTESP QSSGSGSRPV AFSVDPCEEY NDRNKLNTRT
160 170 180 190 200
QHWTCSICTY ENWAKAKKCV VCDHPRPNNI EAIEFAETEE ASSIINEQDR
210 220 230 240 250
ARWRGSCSSG NSQRRSPPTM KRDSEVKMDF QRIELAGAVG SKEELEVDFK
260 270 280 290 300
KLKQIKNRMK KTDWLFLNAC VGVVEGDLAA IEAYKSSGGD IARQLTADEV
310 320 330 340 350
RLLNRPSAFD VGYTLVHLAI RFQRQDMLAI LLTEVSQQAA KCIPAMVCPE
360 370 380 390 400
LTEQIRREIA ASLHQRKGDF ACYFLTDLVT FTLPADIEDL PPTVQEKLFD
410 420 430 440 450
EVLDRDVQKE LEEESPIINW SLELATRLDS RLYALWNRTA GDCLLDSVLQ
460 470 480 490 500
ATWGIYDKDS VLRKALHDSL HDCSHWFYTR WKDWESWYSQ SFGLHFSLRE
510 520 530 540 550
EQWQEDWAFI LSLASQPGAS LEQTHIFVLA HILRRPIIVY GVKYYKSFRG
560 570 580 590 600
ETLGYTRFQG VYLPLLWEQS FCWKSPIALG YTRGHFSALV AMENDGYGNR
610 620 630 640 650
GAGANLNTDD DVTITFLPLV DSERKLLHVH FLSAQELGNE EQQEKLLREW
660 670 680 690 700
LDCCVTEGGV LVAMQKSSRR RNHPLVTQMV EKWLDRYRQI RPCTSLSDGE

EDEDDEDE
Length:708
Mass (Da):81,047
Last modified:August 21, 2007 - v1
Checksum:i386832570C7AEF03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC149099 mRNA. Translation: AAI49100.1.
RefSeqiNP_001094584.1. NM_001101114.1.
UniGeneiBt.53702.

Genome annotation databases

EnsembliENSBTAT00000004401; ENSBTAP00000004401; ENSBTAG00000003395.
GeneIDi523338.
KEGGibta:523338.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC149099 mRNA. Translation: AAI49100.1.
RefSeqiNP_001094584.1. NM_001101114.1.
UniGeneiBt.53702.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4S1ZX-ray3.03F/G/H/I/J2-33[»]
ProteinModelPortaliA6QP16.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004401.

Protein family/group databases

MEROPSiC64.004.

Proteomic databases

PRIDEiA6QP16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000004401; ENSBTAP00000004401; ENSBTAG00000003395.
GeneIDi523338.
KEGGibta:523338.

Organism-specific databases

CTDi54764.

Phylogenomic databases

eggNOGiNOG253754.
GeneTreeiENSGT00530000062989.
HOGENOMiHOG000006743.
HOVERGENiHBG058978.
InParanoidiA6QP16.
KOiK11862.
OMAiSEMKMDF.
OrthoDBiEOG7R56SB.
TreeFamiTF323312.

Miscellaneous databases

NextBioi20873719.

Family and domain databases

InterProiIPR003323. OTU_dom.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
PF00641. zf-RanBP. 2 hits.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 3 hits.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS01358. ZF_RANBP2_1. 3 hits.
PS50199. ZF_RANBP2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal skin.

Entry informationi

Entry nameiZRAN1_BOVIN
AccessioniPrimary (citable) accession number: A6QP16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: August 21, 2007
Last modified: July 22, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.