Reviewed, UniProtKB/Swiss-Prot A6QP15 (HOT_BOVIN)

Names and origin

Protein names

Recommended name:
    Hydroxyacid-oxoacid transhydrogenase, mitochondrial
      Short name=HOT
    EC=1.1.99.24
Alternative name(s):
    Alcohol dehydrogenase iron-containing protein 1

Gene names

Name:

ADHFE1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG By similarity.
Catalytic activity	(S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate. 2-oxoglutaric acid + 4-hydroxybutanoic acid = (R)-2-hydroxyglutaric acid + succinic semialdehyde.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	molecular hydrogen transport Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	hydroxyacid-oxoacid transhydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 466		Hydroxyacid-oxoacid transhydrogenase, mitochondrial		PRO_0000322995

Sequences

	Sequence		Length	Mass (Da)
	A6QP15-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: EB601252CBE6DA6E	FASTA	466	50,343
	10 20 30 40 50 60 MAAARSRVVH LLRLLQRAAC QCPSHSHTYS QAPGLSPSGK TTDYAFEMAV STIRYGAGVT 70 80 90 100 110 120 KEVGMDLQSM GAKNVCLMTD KNLSQLPPVQ TVMDSLVKNG INFKVYDHVR VEPTDTSFME 130 140 150 160 170 180 AIEFAKKGAF DAFLAVGGGS TIDTCKAANL YSSSPDSDFL DYVNAPIGKG KPVTVPLKPL 190 200 210 220 230 240 IAVPTTSGTG SETTGVAIFD YEHLKVKTGI ASRAIKPTLG LIDPLHTLHM PERVVANSGF 250 260 270 280 290 300 DVLCHALESY TALPYHMRSP CPSSPITRPA YQGSNPISDI WAVHALRIVA KYLKRAIRNP 310 320 330 340 350 360 DDLEARSNMH LASAFAGIGF GNAGVHLCHG MSYPISGLVK TYKAKDYNVD HPLVPHGLSV 370 380 390 400 410 420 VLTSPAVFTF TSQMFPERHL EVAEILGADT RTARRPDAGP VLADTLRKFL FDLDVDDGLA 430 440 450 460 AIGYSKADIP ELVKGTLPQE RVTKLAPRPQ SEEDLSALFE ASMKLY

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC149097 mRNA. Translation: AAI49098.1.
IPI	IPI00867494.
RefSeq	NP_001095357.1.
UniGene	Bt.47646
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000004476. Bos taurus. [Contig view]
GeneID	507711.
KEGG	bta:507711.
Enzyme and pathway databases
BRENDA	1.1.99.24. 251.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. False negative. PS00060. ADH_IRON_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOT_BOVIN

Accession

Primary (citable) accession number: A6QP15

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	August 21, 2007
Last modified:	June 16, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)