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A6QNM7

- UBP33_BOVIN

UniProt

A6QNM7 - UBP33_BOVIN

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Protein
Ubiquitin carboxyl-terminal hydrolase 33
Gene
USP33
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei163 – 1631Nucleophile By similarity
Active sitei643 – 6431Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-type
Add
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. ubiquitin thiolesterase activity Source: UniProtKB
  3. ubiquitin-specific protease activity Source: UniProtKB
  4. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. axon guidance Source: UniProtKB
  2. cell migration Source: UniProtKB
  3. centrosome duplication Source: UniProtKB
  4. endocytosis Source: UniProtKB-KW
  5. protein K48-linked deubiquitination Source: UniProtKB
  6. protein K63-linked deubiquitination Source: UniProtKB
  7. protein deubiquitination Source: UniProtKB
  8. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
  9. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Endocytosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.037.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 33 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 33
Ubiquitin thioesterase 33
Ubiquitin-specific-processing protease 33
Gene namesi
Name:USP33
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 3

Subcellular locationi

Cytoplasmperinuclear region By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
Note: Associates with centrosomes predominantly in S and G2 phases but less in G1 phase By similarity.

GO - Cellular componenti

  1. cell body Source: Ensembl
  2. centrosome Source: UniProtKB
  3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 912912Ubiquitin carboxyl-terminal hydrolase 33
PRO_0000390423Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei408 – 4081Phosphoserine By similarity

Post-translational modificationi

Ubiquitinated via a VHL-dependent pathway for proteasomal degradation By similarity.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Interacts with VHL, leading to its ubiquitination and subsequent degradation. Interacts with ARRB1, ARRB2, ADRB2, DIO2 and ROBO1. Interacts with SELENBP1; in a selenium-dependent manner. Interacts with CCP110 By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027667.

Structurei

3D structure databases

ProteinModelPortaliA6QNM7.
SMRiA6QNM7. Positions 5-99.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini154 – 685532USP
Add
BLAST
Domaini687 – 78094DUSP 1
Add
BLAST
Domaini788 – 891104DUSP 2
Add
BLAST

Domaini

The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue By similarity.

Sequence similaritiesi

Contains 2 DUSP domains.
Contains 1 USP domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00750000117701.
HOGENOMiHOG000286031.
HOVERGENiHBG054196.
InParanoidiA6QNM7.
KOiK11848.
OMAiLPSNEGV.
OrthoDBiEOG7CRTP2.
TreeFamiTF352179.

Family and domain databases

Gene3Di3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF06337. DUSP. 1 hit.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 2 hits.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 2 hits.
PROSITEiPS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QNM7-1 [UniParc]FASTAAdd to Basket

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MSSFRSHCPH LDSVGEITKE DLIQKSHGSC QDCKVRGPNL WACLENRCSY    50
VGCGESQVDH STIHSQETKH YLTVNLTTLR VWCYACSKEV FLDRKLGTQP 100
SLPHVKPLHQ IQENGVQDFK IPSNTTLKTP LVAVFDDLDI EVEEEDELKA 150
RGLTGLKNIG NTCYMNAALQ ALSNCPPLTQ FFLDCGGLAR TDKKPAICKS 200
YLKLMTELWH KSRPGSVVPT TLFQGIKTVN PTFRGYSQQD AQEFLRCLMD 250
LLHEELKEQV MEVEEDPQTI MTEETMEEDK SQSDVDFQSC ESCSSSDKAE 300
NENGSRSFSE DNNETTMLIQ DDENNSEMSK DWQKEKMCNK INKVHSEGEL 350
DKDRDSVSET ADLNNQETVK VQIHSRASEY ITDVHLNDLS TPQILPSNEG 400
VNPRLSASPP KSGNLWPGLP PTHKKVQSAL SPKRKKQHKK YRSVISDIFD 450
GTIISSVQCL TCDRVSVTLE TFQDLSLPIP GKEDLAKLHS SSHPTSIVKA 500
GSCGEAYAPQ GWIAFFMEYV KRFVVSCVPS WFWGPVVTLQ DCLAAFFARD 550
ELKGDNMYSC EKCKKLRNGV KFCKVQKFPE ILCIHLKRFR HELMFSTKIS 600
THVSFPLEGL DLQPFLAKDS PVQIVTYDLL SVICHHGTAS SGHYIAYCRN 650
NLNNLWYEFD DQSVTEVSES TVQNAEAYVL FYRKSSEEAQ KERRRISNLL 700
NIMEPSLLQF YISRQWLNKF KTFAEPGPIS NNDFLCIHGG VPPRKAGYIE 750
DLVLMLPQNI WDNLYSRYGG GPAVNHLYIC HTCQIEAEKI EKRRKTELEI 800
FIRLNRAFQE EDSPATFYCI SMQWFREWES FVKGKDGDPP GPIDNTKIAV 850
TKCGNVILRQ GADSGQISEE TWNFLQSIYG GGPEVILRPP VVHVDPDAVQ 900
AEEKIEVETR SL 912
Length:912
Mass (Da):103,323
Last modified:August 21, 2007 - v1
Checksum:iAF5F91718BA407B4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC148905 mRNA. Translation: AAI48906.1.
RefSeqiNP_001094609.1. NM_001101139.2.
UniGeneiBt.46098.

Genome annotation databases

EnsembliENSBTAT00000027667; ENSBTAP00000027667; ENSBTAG00000020761.
GeneIDi531706.
KEGGibta:531706.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC148905 mRNA. Translation: AAI48906.1 .
RefSeqi NP_001094609.1. NM_001101139.2.
UniGenei Bt.46098.

3D structure databases

ProteinModelPortali A6QNM7.
SMRi A6QNM7. Positions 5-99.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000027667.

Protein family/group databases

MEROPSi C19.037.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000027667 ; ENSBTAP00000027667 ; ENSBTAG00000020761 .
GeneIDi 531706.
KEGGi bta:531706.

Organism-specific databases

CTDi 23032.

Phylogenomic databases

eggNOGi COG5560.
GeneTreei ENSGT00750000117701.
HOGENOMi HOG000286031.
HOVERGENi HBG054196.
InParanoidi A6QNM7.
KOi K11848.
OMAi LPSNEGV.
OrthoDBi EOG7CRTP2.
TreeFami TF352179.

Miscellaneous databases

NextBioi 20875513.

Family and domain databases

Gene3Di 3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProi IPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF06337. DUSP. 1 hit.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
SMARTi SM00695. DUSP. 2 hits.
[Graphical view ]
SUPFAMi SSF143791. SSF143791. 2 hits.
PROSITEi PS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.

Entry informationi

Entry nameiUBP33_BOVIN
AccessioniPrimary (citable) accession number: A6QNM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: August 21, 2007
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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