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A6QNM7

- UBP33_BOVIN

UniProt

A6QNM7 - UBP33_BOVIN

Protein

Ubiquitin carboxyl-terminal hydrolase 33

Gene

USP33

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei163 – 1631NucleophilePROSITE-ProRule annotation
    Active sitei643 – 6431Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: UniProtKB
    2. ubiquitin-specific protease activity Source: UniProtKB
    3. ubiquitin thiolesterase activity Source: UniProtKB
    4. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. axon guidance Source: UniProtKB
    2. cell migration Source: UniProtKB
    3. centrosome duplication Source: UniProtKB
    4. endocytosis Source: UniProtKB-KW
    5. protein deubiquitination Source: UniProtKB
    6. protein K48-linked deubiquitination Source: UniProtKB
    7. protein K63-linked deubiquitination Source: UniProtKB
    8. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
    9. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Endocytosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC19.037.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 33 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 33
    Ubiquitin thioesterase 33
    Ubiquitin-specific-processing protease 33
    Gene namesi
    Name:USP33
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 3

    Subcellular locationi

    Cytoplasmperinuclear region By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
    Note: Associates with centrosomes predominantly in S and G2 phases but less in G1 phase.By similarity

    GO - Cellular componenti

    1. cell body Source: Ensembl
    2. centrosome Source: UniProtKB
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 912912Ubiquitin carboxyl-terminal hydrolase 33PRO_0000390423Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei408 – 4081PhosphoserineBy similarity

    Post-translational modificationi

    Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Interactioni

    Subunit structurei

    Interacts with VHL, leading to its ubiquitination and subsequent degradation. Interacts with ARRB1, ARRB2, ADRB2, DIO2 and ROBO1. Interacts with SELENBP1; in a selenium-dependent manner. Interacts with CCP110 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000027667.

    Structurei

    3D structure databases

    ProteinModelPortaliA6QNM7.
    SMRiA6QNM7. Positions 5-99.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini154 – 685532USPAdd
    BLAST
    Domaini687 – 78094DUSP 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini788 – 891104DUSP 2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue By similarity.By similarity

    Sequence similaritiesi

    Contains 2 DUSP domains.PROSITE-ProRule annotation
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5560.
    GeneTreeiENSGT00750000117701.
    HOGENOMiHOG000286031.
    HOVERGENiHBG054196.
    InParanoidiA6QNM7.
    KOiK11848.
    OMAiLPSNEGV.
    OrthoDBiEOG7CRTP2.
    TreeFamiTF352179.

    Family and domain databases

    Gene3Di3.30.2230.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProiIPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF06337. DUSP. 1 hit.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    SMARTiSM00695. DUSP. 2 hits.
    [Graphical view]
    SUPFAMiSSF143791. SSF143791. 2 hits.
    PROSITEiPS51283. DUSP. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A6QNM7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSFRSHCPH LDSVGEITKE DLIQKSHGSC QDCKVRGPNL WACLENRCSY    50
    VGCGESQVDH STIHSQETKH YLTVNLTTLR VWCYACSKEV FLDRKLGTQP 100
    SLPHVKPLHQ IQENGVQDFK IPSNTTLKTP LVAVFDDLDI EVEEEDELKA 150
    RGLTGLKNIG NTCYMNAALQ ALSNCPPLTQ FFLDCGGLAR TDKKPAICKS 200
    YLKLMTELWH KSRPGSVVPT TLFQGIKTVN PTFRGYSQQD AQEFLRCLMD 250
    LLHEELKEQV MEVEEDPQTI MTEETMEEDK SQSDVDFQSC ESCSSSDKAE 300
    NENGSRSFSE DNNETTMLIQ DDENNSEMSK DWQKEKMCNK INKVHSEGEL 350
    DKDRDSVSET ADLNNQETVK VQIHSRASEY ITDVHLNDLS TPQILPSNEG 400
    VNPRLSASPP KSGNLWPGLP PTHKKVQSAL SPKRKKQHKK YRSVISDIFD 450
    GTIISSVQCL TCDRVSVTLE TFQDLSLPIP GKEDLAKLHS SSHPTSIVKA 500
    GSCGEAYAPQ GWIAFFMEYV KRFVVSCVPS WFWGPVVTLQ DCLAAFFARD 550
    ELKGDNMYSC EKCKKLRNGV KFCKVQKFPE ILCIHLKRFR HELMFSTKIS 600
    THVSFPLEGL DLQPFLAKDS PVQIVTYDLL SVICHHGTAS SGHYIAYCRN 650
    NLNNLWYEFD DQSVTEVSES TVQNAEAYVL FYRKSSEEAQ KERRRISNLL 700
    NIMEPSLLQF YISRQWLNKF KTFAEPGPIS NNDFLCIHGG VPPRKAGYIE 750
    DLVLMLPQNI WDNLYSRYGG GPAVNHLYIC HTCQIEAEKI EKRRKTELEI 800
    FIRLNRAFQE EDSPATFYCI SMQWFREWES FVKGKDGDPP GPIDNTKIAV 850
    TKCGNVILRQ GADSGQISEE TWNFLQSIYG GGPEVILRPP VVHVDPDAVQ 900
    AEEKIEVETR SL 912
    Length:912
    Mass (Da):103,323
    Last modified:August 21, 2007 - v1
    Checksum:iAF5F91718BA407B4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC148905 mRNA. Translation: AAI48906.1.
    RefSeqiNP_001094609.1. NM_001101139.2.
    UniGeneiBt.46098.

    Genome annotation databases

    EnsembliENSBTAT00000027667; ENSBTAP00000027667; ENSBTAG00000020761.
    GeneIDi531706.
    KEGGibta:531706.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC148905 mRNA. Translation: AAI48906.1 .
    RefSeqi NP_001094609.1. NM_001101139.2.
    UniGenei Bt.46098.

    3D structure databases

    ProteinModelPortali A6QNM7.
    SMRi A6QNM7. Positions 5-99.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000027667.

    Protein family/group databases

    MEROPSi C19.037.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000027667 ; ENSBTAP00000027667 ; ENSBTAG00000020761 .
    GeneIDi 531706.
    KEGGi bta:531706.

    Organism-specific databases

    CTDi 23032.

    Phylogenomic databases

    eggNOGi COG5560.
    GeneTreei ENSGT00750000117701.
    HOGENOMi HOG000286031.
    HOVERGENi HBG054196.
    InParanoidi A6QNM7.
    KOi K11848.
    OMAi LPSNEGV.
    OrthoDBi EOG7CRTP2.
    TreeFami TF352179.

    Miscellaneous databases

    NextBioi 20875513.

    Family and domain databases

    Gene3Di 3.30.2230.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProi IPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF06337. DUSP. 1 hit.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    SMARTi SM00695. DUSP. 2 hits.
    [Graphical view ]
    SUPFAMi SSF143791. SSF143791. 2 hits.
    PROSITEi PS51283. DUSP. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Hypothalamus.

    Entry informationi

    Entry nameiUBP33_BOVIN
    AccessioniPrimary (citable) accession number: A6QNM7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3