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A6QNM7

- UBP33_BOVIN

UniProt

A6QNM7 - UBP33_BOVIN

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Protein

Ubiquitin carboxyl-terminal hydrolase 33

Gene

USP33

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei163 – 1631NucleophilePROSITE-ProRule annotation
Active sitei643 – 6431Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. ubiquitin-specific protease activity Source: UniProtKB
  3. ubiquitin thiolesterase activity Source: UniProtKB
  4. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. axon guidance Source: UniProtKB
  2. cell migration Source: UniProtKB
  3. centrosome duplication Source: UniProtKB
  4. endocytosis Source: UniProtKB-KW
  5. protein deubiquitination Source: UniProtKB
  6. protein K48-linked deubiquitination Source: UniProtKB
  7. protein K63-linked deubiquitination Source: UniProtKB
  8. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
  9. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Endocytosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.037.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 33 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 33
Ubiquitin thioesterase 33
Ubiquitin-specific-processing protease 33
Gene namesi
Name:USP33
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 3

Subcellular locationi

Cytoplasmperinuclear region By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
Note: Associates with centrosomes predominantly in S and G2 phases but less in G1 phase.By similarity

GO - Cellular componenti

  1. cell body Source: Ensembl
  2. centrosome Source: UniProtKB
  3. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 912912Ubiquitin carboxyl-terminal hydrolase 33PRO_0000390423Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei408 – 4081PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Interacts with VHL, leading to its ubiquitination and subsequent degradation. Interacts with ARRB1, ARRB2, ADRB2, DIO2 and ROBO1. Interacts with SELENBP1; in a selenium-dependent manner. Interacts with CCP110 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027667.

Structurei

3D structure databases

ProteinModelPortaliA6QNM7.
SMRiA6QNM7. Positions 5-99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini154 – 685532USPAdd
BLAST
Domaini687 – 78094DUSP 1PROSITE-ProRule annotationAdd
BLAST
Domaini788 – 891104DUSP 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue (By similarity).By similarity

Sequence similaritiesi

Contains 2 DUSP domains.PROSITE-ProRule annotation
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00760000119208.
HOGENOMiHOG000286031.
HOVERGENiHBG054196.
InParanoidiA6QNM7.
KOiK11848.
OMAiLPSNEGV.
OrthoDBiEOG7CRTP2.
TreeFamiTF352179.

Family and domain databases

Gene3Di3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF06337. DUSP. 1 hit.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 2 hits.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 2 hits.
PROSITEiPS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QNM7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSFRSHCPH LDSVGEITKE DLIQKSHGSC QDCKVRGPNL WACLENRCSY
60 70 80 90 100
VGCGESQVDH STIHSQETKH YLTVNLTTLR VWCYACSKEV FLDRKLGTQP
110 120 130 140 150
SLPHVKPLHQ IQENGVQDFK IPSNTTLKTP LVAVFDDLDI EVEEEDELKA
160 170 180 190 200
RGLTGLKNIG NTCYMNAALQ ALSNCPPLTQ FFLDCGGLAR TDKKPAICKS
210 220 230 240 250
YLKLMTELWH KSRPGSVVPT TLFQGIKTVN PTFRGYSQQD AQEFLRCLMD
260 270 280 290 300
LLHEELKEQV MEVEEDPQTI MTEETMEEDK SQSDVDFQSC ESCSSSDKAE
310 320 330 340 350
NENGSRSFSE DNNETTMLIQ DDENNSEMSK DWQKEKMCNK INKVHSEGEL
360 370 380 390 400
DKDRDSVSET ADLNNQETVK VQIHSRASEY ITDVHLNDLS TPQILPSNEG
410 420 430 440 450
VNPRLSASPP KSGNLWPGLP PTHKKVQSAL SPKRKKQHKK YRSVISDIFD
460 470 480 490 500
GTIISSVQCL TCDRVSVTLE TFQDLSLPIP GKEDLAKLHS SSHPTSIVKA
510 520 530 540 550
GSCGEAYAPQ GWIAFFMEYV KRFVVSCVPS WFWGPVVTLQ DCLAAFFARD
560 570 580 590 600
ELKGDNMYSC EKCKKLRNGV KFCKVQKFPE ILCIHLKRFR HELMFSTKIS
610 620 630 640 650
THVSFPLEGL DLQPFLAKDS PVQIVTYDLL SVICHHGTAS SGHYIAYCRN
660 670 680 690 700
NLNNLWYEFD DQSVTEVSES TVQNAEAYVL FYRKSSEEAQ KERRRISNLL
710 720 730 740 750
NIMEPSLLQF YISRQWLNKF KTFAEPGPIS NNDFLCIHGG VPPRKAGYIE
760 770 780 790 800
DLVLMLPQNI WDNLYSRYGG GPAVNHLYIC HTCQIEAEKI EKRRKTELEI
810 820 830 840 850
FIRLNRAFQE EDSPATFYCI SMQWFREWES FVKGKDGDPP GPIDNTKIAV
860 870 880 890 900
TKCGNVILRQ GADSGQISEE TWNFLQSIYG GGPEVILRPP VVHVDPDAVQ
910
AEEKIEVETR SL
Length:912
Mass (Da):103,323
Last modified:August 21, 2007 - v1
Checksum:iAF5F91718BA407B4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC148905 mRNA. Translation: AAI48906.1.
RefSeqiNP_001094609.1. NM_001101139.2.
UniGeneiBt.46098.

Genome annotation databases

EnsembliENSBTAT00000027667; ENSBTAP00000027667; ENSBTAG00000020761.
GeneIDi531706.
KEGGibta:531706.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC148905 mRNA. Translation: AAI48906.1 .
RefSeqi NP_001094609.1. NM_001101139.2.
UniGenei Bt.46098.

3D structure databases

ProteinModelPortali A6QNM7.
SMRi A6QNM7. Positions 5-99.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000027667.

Protein family/group databases

MEROPSi C19.037.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000027667 ; ENSBTAP00000027667 ; ENSBTAG00000020761 .
GeneIDi 531706.
KEGGi bta:531706.

Organism-specific databases

CTDi 23032.

Phylogenomic databases

eggNOGi COG5560.
GeneTreei ENSGT00760000119208.
HOGENOMi HOG000286031.
HOVERGENi HBG054196.
InParanoidi A6QNM7.
KOi K11848.
OMAi LPSNEGV.
OrthoDBi EOG7CRTP2.
TreeFami TF352179.

Miscellaneous databases

NextBioi 20875513.

Family and domain databases

Gene3Di 3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProi IPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF06337. DUSP. 1 hit.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
SMARTi SM00695. DUSP. 2 hits.
[Graphical view ]
SUPFAMi SSF143791. SSF143791. 2 hits.
PROSITEi PS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.

Entry informationi

Entry nameiUBP33_BOVIN
AccessioniPrimary (citable) accession number: A6QNM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: August 21, 2007
Last modified: October 29, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3