A6QNK1 (G3ST1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactosylceramide sulfotransferase
Short name=GalCer sulfotransferase
EC=2.8.2.11

Alternative name(s):
3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase
3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase
Cerebroside sulfotransferase

Gene names

Name:

GAL3ST1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the sulfation of membrane glycolipids. Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety. Catalyzes the synthesis of HSO3-3-galactosylceramide (sulfatide), a major lipid component of the myelin sheath and of HSO3-3-monogalactosylalkylacylglycerol (seminolipid), present in spermatocytes. Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro) By similarity.
Catalytic activity	3'-phosphoadenylyl sulfate + a galactosylceramide = adenosine 3',5'-bisphosphate + a galactosylceramidesulfate. 3'-phosphoadenylyl sulfate + monogalactosylalkylacylglycerol = adenosine 3',5'-bisphosphate + monogalactosylalkylacylglycerol sulfate.
Pathway	Lipid metabolism; sphingolipid metabolism.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the galactose-3-O-sulfotransferase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosylceramide sulfotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Galactosylceramide sulfotransferase		PRO_0000315388
Regions
	Topological domain	1 – 14	14	Cytoplasmic Potential
	Transmembrane	15 – 35	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	36 – 423	388	Lumenal Potential
Amino acid modifications
	Glycosylation	66	1	N-linked (GlcNAc...) Potential
	Glycosylation	156	1	N-linked (GlcNAc...) Potential
	Glycosylation	312	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A6QNK1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: C987BABD661282A7	FASTA	423	48,754
	10 20 30 40 50 60 MPLPQKKRWE SMAKGLVLGA LFTSFLLLLY SYAVPPLYTG LASTTPEGAA PCSPAPREPE 70 80 90 100 110 120 APTSANGSAG GCQPRRDIVF MKTHKTASST LLNILFRFGQ KHGLKFAFPN GRNDFDYPAF 130 140 150 160 170 180 FARSLVQDYR PGACFNIICN HMRFHYDEVR GLVAPNATFI TVLRDPARLF ESSFHYFGSV 190 200 210 220 230 240 VPFTWKLSGR DKLAEFLQDP DRYYDARGYN AHYLRNLLFF DLGYDSDLDP SSPQVQEHIL 250 260 270 280 290 300 EVERHFHLVL LQEYFDESLV LLKDLLCWEL EDVLYFKLNA RRASAVPRLS GELYRRATAW 310 320 330 340 350 360 NVLDARLYRH FNASFWRKVE AFGRERMARE VAALRRANER MRRICIDGGR AVDAAAIEDS 370 380 390 400 410 420 AMQPWQPLGA KSILGYNLKK SIGQRHAQLC RRMLTPEIQY LMDLGANLWI TKLWKFIRDF LRW

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC148870 mRNA. Translation: AAI48871.1.
IPI	IPI00867250.
RefSeq	NP_001095442.1. NM_001101972.1.
UniGene	Bt.77813.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6QNK1.
Proteomic databases
PRIDE	A6QNK1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	513295.
KEGG	bta:513295.
Organism-specific databases
CTD	9514.
Phylogenomic databases
eggNOG	maNOG04943.
GeneTree	ENSGT00390000013505.
HOVERGEN	HBG051681.
InParanoid	A6QNK1.
OrthoDB	EOG48D0VH.
Family and domain databases
InterPro	IPR009729. Gal-3-0_sulfotransfrase. [Graphical view]
KO	K01019.
PANTHER	PTHR14647. Gal-3-0_sulfotr. 1 hit.
Pfam	PF06990. Gal-3-0_sulfotr. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3ST1_BOVIN

Accession

Primary (citable) accession number: A6QNK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 21, 2007
Last modified:	November 16, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program