A6QLU1 (GPDM_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate dehydrogenase, mitochondrial
Short name=GPD-M
Short name=GPDH-M
EC=1.1.5.3

Gene names

Name:

GPD2

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.
Cofactor	FAD By similarity.
Enzyme regulation	Calcium-binding enhance the activity of the enzyme By similarity.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (aerobic route): step 1/1.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Repeat Transit peptide
Ligand	Calcium FAD Flavoprotein Metal-binding
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	gluconeogenesis Inferred from electronic annotation. Source: Compara glycerol catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro mitochondrial inner membrane Inferred from electronic annotation. Source: Compara
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 42	42	Mitochondrion By similarity
	Chain	43 – 727	685	Glycerol-3-phosphate dehydrogenase, mitochondrial		PRO_0000355966
Regions
	Domain	623 – 658	36	EF-hand 1
	Domain	659 – 694	36	EF-hand 2
	Nucleotide binding	71 – 99	29	FAD Potential
	Calcium binding	672 – 683	12	Potential
Amino acid modifications
	Modified residue	600	1	Phosphotyrosine By similarity
	Modified residue	601	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6QLU1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: 1F02904CEEA3CC71	FASTA	727	80,763
	10 20 30 40 50 60 MAFQKAVKGT ILVGGGALAT VLGLSHFAHY KRKQVNLAFV EAADCISEPV NREPPSREAQ 70 80 90 100 110 120 ILTLKNTSEF DVLVIGGGAT GSGCALDAVT RGLKTALVER DDFSSGTSSR STKLIHGGVR 130 140 150 160 170 180 YLQKAIMKLD IEQYRMVKEA LHERANLLEI APHLSAPLPI MLPIYKWWQL PYYWVGIKLY 190 200 210 220 230 240 DLVAGSNCLK SSYVLSKSRA LEHFPMLQKD KLVGAIVYYD GQHNDARMNL AIALTAARYG 250 260 270 280 290 300 AATANYMEVM SLLKKTDPQT GKERVSGARC KDVLTGEEFD VRAKCVINAT GPFTDTVRKM 310 320 330 340 350 360 DDKDTTAICQ PSAGVHIVMP GYYSPESMGL LDPATSDGRV IFFLPWQKMT IAGTTDTPTD 370 380 390 400 410 420 VTHHPIPSEE DINFILNEVR NYLSCDVEVR RGDVLAAWSG IRPLVTDPKS ADTKSISRNH 430 440 450 460 470 480 VVDISESGLI TIAGGKWTTY RSMAEDTINA AVKAHNLKAG PSRTVGLFLQ GGKDWSPTLY 490 500 510 520 530 540 IRLVQDYGLE SEVAQHLAAT YGDKAFEVAK MASVTGKRWP IVGVRLVSEF PYIEAEVKYG 550 560 570 580 590 600 IKEYACTAVD MISRRTRLAF LNVQAAEEAL PRIVELMGRE LNWDDSKKEE ELETARKFLY 610 620 630 640 650 660 YEMGYKSRSE QLTDRSEISL LPSDIDRYKK RFHKFDADQK GFITIVDVQR VLESIGVQMD 670 680 690 700 710 720 ENTLHEILNE VDLNKNGQVE LNEFLQLMSA IQKGRVSGSR LAILMKTAEE NLDRRVPIPV DRSCGGL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC148085 mRNA. Translation: AAI48086.1.
IPI	IPI00692457.
RefSeq	NP_001093766.1. NM_001100296.1.
UniGene	Bt.48365.
3D structure databases
ProteinModelPortal	A6QLU1.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000012886.
Proteomic databases
PRIDE	A6QLU1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000012886; ENSBTAP00000012886; ENSBTAG00000009770.
GeneID	504948.
KEGG	bta:504948.
Organism-specific databases
CTD	2820.
Phylogenomic databases
eggNOG	COG0578.
GeneTree	ENSGT00390000001718.
HOGENOM	HOG000004813.
HOVERGEN	HBG005897.
InParanoid	A6QLU1.
KO	K00111.
OMA	DSIRKMD.
OrthoDB	EOG4PC9RN.
Enzyme and pathway databases
UniPathway	UPA00618; UER00674.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR006076. FAD-dep_OxRdtase. IPR000447. G3P_DH_FAD-dep. [Graphical view]
Pfam	PF01266. DAO. 1 hit. PF13499. EF_hand_5. 1 hit. [Graphical view]
PRINTS	PR01001. FADG3PDH.
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. PS00977. FAD_G3PDH_1. 1 hit. PS00978. FAD_G3PDH_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20866901.

Entry information

Entry name

GPDM_BOVIN

Accession

Primary (citable) accession number: A6QLU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	August 21, 2007
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program