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Protein

5-aminolevulinate synthase, nonspecific, mitochondrial

Gene

ALAS1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactori

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from glycine.
Proteins known to be involved in this subpathway in this organism are:
  1. 5-aminolevulinate synthase, nonspecific, mitochondrial (ALAS1), 5-aminolevulinate synthase, erythroid-specific, mitochondrial (ALAS2)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from glycine, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei224SubstrateBy similarity1
Binding sitei341SubstrateBy similarity1
Binding sitei360SubstrateBy similarity1
Binding sitei393Pyridoxal phosphateBy similarity1
Binding sitei421Pyridoxal phosphateBy similarity1
Binding sitei449Pyridoxal phosphateBy similarity1
Active sitei452By similarity1
Binding sitei481Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei482Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei569SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Heme biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-BTA-189451. Heme biosynthesis.
UniPathwayiUPA00251; UER00375.

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, nonspecific, mitochondrial (EC:2.3.1.37)
Short name:
ALAS-H
Alternative name(s):
5-aminolevulinic acid synthase 1
Delta-ALA synthase 1
Delta-aminolevulinate synthase 1
Gene namesi
Name:ALAS1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 22

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 56MitochondrionBy similarityAdd BLAST56
ChainiPRO_000035267657 – 6475-aminolevulinate synthase, nonspecific, mitochondrialAdd BLAST591

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei452N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiA6QLI6.
PRIDEiA6QLI6.

Expressioni

Gene expression databases

BgeeiENSBTAG00000004118.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000005380.

Structurei

3D structure databases

ProteinModelPortaliA6QLI6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1360. Eukaryota.
COG0156. LUCA.
GeneTreeiENSGT00530000063111.
HOGENOMiHOG000221020.
HOVERGENiHBG005954.
InParanoidiA6QLI6.
KOiK00643.
OMAiPQMMSYF.
OrthoDBiEOG091G043U.
TreeFamiTF300724.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6QLI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METVVRRCPF LSRVPQAFLQ KAGKSLLFYA QNCPKMMEIG AKPAPRALST
60 70 80 90 100
SAVLCQQVTE TPPANEKDKA AKAEVQQAPD GSQQAPDGSQ QTADGTQLPS
110 120 130 140 150
GHPSLASSQG TGSKCPFLAA EMSQGGSSVF RKASLALQED VQEMHAVREE
160 170 180 190 200
VAQTSVNPSV INVKTEGGEL NGLLKNFQDI MRKQRPERVS HLLQDNLPKS
210 220 230 240 250
VCTFQYDRFF EKKIDEKKND HSYRVFKTVN RKAQCFPMAD DYSDSLISKK
260 270 280 290 300
QVSVWCSNDY LGMSRHPRVC GAVIDTLKQH GTGAGGTRNI SGTSKFHVDL
310 320 330 340 350
EQELADLHGK DAALLFSSCF VANDSTLFTL AKMMPGCEIY SDAGNHASMI
360 370 380 390 400
QGIRNSGVPK YIFRHNDVSH LRELLQRSDP AVPKIVAFET VHSMDGAVCP
410 420 430 440 450
LEELCDVAHE FGAITFVDEV HAVGLYGLQG GGIGDRDGVM PKMDIISGTL
460 470 480 490 500
GKAIGCVGGY IASTSSLIDT VRSYAAGFIF TTSLPPMLLA GALESVRILR
510 520 530 540 550
STEGRTLRRQ HQRNVKLMRQ MLMDAGLPVV HCPSHIIPVR VADAAKNTEV
560 570 580 590 600
CDELMTRHNI YVQAINYPTV PRGEELLRIA PTPHHTPQMM SYFVDNLLAT
610 620 630 640
WKRVGLELKP HSSAECNFCR RPLHFEMMSE REKSYFSGMS KLVSAQA
Length:647
Mass (Da):71,062
Last modified:August 21, 2007 - v1
Checksum:iDF0A4622352C9401
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC147978 mRNA. Translation: AAI47979.1.
RefSeqiNP_001094624.1. NM_001101154.1.
UniGeneiBt.15857.

Genome annotation databases

EnsembliENSBTAT00000005380; ENSBTAP00000005380; ENSBTAG00000004118.
GeneIDi534286.
KEGGibta:534286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC147978 mRNA. Translation: AAI47979.1.
RefSeqiNP_001094624.1. NM_001101154.1.
UniGeneiBt.15857.

3D structure databases

ProteinModelPortaliA6QLI6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000005380.

Proteomic databases

PaxDbiA6QLI6.
PRIDEiA6QLI6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000005380; ENSBTAP00000005380; ENSBTAG00000004118.
GeneIDi534286.
KEGGibta:534286.

Organism-specific databases

CTDi211.

Phylogenomic databases

eggNOGiKOG1360. Eukaryota.
COG0156. LUCA.
GeneTreeiENSGT00530000063111.
HOGENOMiHOG000221020.
HOVERGENiHBG005954.
InParanoidiA6QLI6.
KOiK00643.
OMAiPQMMSYF.
OrthoDBiEOG091G043U.
TreeFamiTF300724.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.
ReactomeiR-BTA-189451. Heme biosynthesis.

Gene expression databases

BgeeiENSBTAG00000004118.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEM1_BOVIN
AccessioniPrimary (citable) accession number: A6QLI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: August 21, 2007
Last modified: September 7, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.