Reviewed, UniProtKB/Swiss-Prot A6QLA4 (AMPM1_BOVIN)

Names and origin

Protein names

Recommended name:
    Methionine aminopeptidase 1
      Short name=MetAP 1
      Short name=MAP 1
    EC=3.4.11.18
Alternative name(s):
    Peptidase M 1

Gene names

Name:

METAP1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity. Binds 1 sodium ion per subunit. The sodium ion has a structural role By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	Methionine aminopeptidase 1		PRO_0000323734
Sites
	Metal binding	220	1	Cobalt 1 By similarity
	Metal binding	231	1	Cobalt 1 By similarity
	Metal binding	231	1	Cobalt 2 By similarity
	Metal binding	294	1	Cobalt 2 By similarity
	Metal binding	327	1	Cobalt 2 By similarity
	Metal binding	358	1	Cobalt 1 By similarity
	Metal binding	358	1	Cobalt 2 By similarity
	Binding site	203	1	Substrate By similarity
	Binding site	301	1	Substrate By similarity
Amino acid modifications
	Modified residue	253	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6QLA4-1 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: A8B81D71B11C04C2	FASTA	386	43,183
	10 20 30 40 50 60 MAAVETRVCE TDGCSSEAKL QCPTCIKLGI QGSYFCSQEC FKGSWATHKL LHKKAKDEKA 70 80 90 100 110 120 KREVSSWTVE GDINTDPWAG YRYTGKLRPH YPLMPTRPVP SYIQRPDYAD HPLGMSESEQ 130 140 150 160 170 180 ALKGTSQIKL LSSEDIEGMR LVCRLAREVL DIAAGLIKPG VTTEEIDHAV HLACIARNCY 190 200 210 220 230 240 PSPLNYYNFP KSCCTSVNEV ICHGIPDRRP LQEGDIVNVD ITLYRNGYHG DLNETFFVGD 250 260 270 280 290 300 VDEGARKLVQ TTYECLMQAI DAVKPGVRYR ELGNIIQKHA QANGFSVVRS YCGHGIHKLF 310 320 330 340 350 360 HTAPNVPHYS KNKAVGVMKA GHVFTIEPMI CEGGWQDETW PDGWTAVTRD GKRSAQFEHT 370 380 LLVTDTGCEI LTRRLDSAQP HFMSQF

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Heart ventricle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC147894 mRNA. Translation: AAI47895.1.
IPI	IPI00700958.
RefSeq	NP_001095507.1.
UniGene	Bt.41964
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000021303; ENSBTAP00000021303; ENSBTAG00000016004; Bos taurus. [Genome view] ENSBTAT00000048306; ENSBTAP00000045369; ENSBTAG00000016004; Bos taurus. [Genome view]
GeneID	516540.
KEGG	bta:516540.
Organism-specific databases
CTD	516540.
Enzyme and pathway databases
BRENDA	3.4.11.18. 251.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHER	PTHR10804:SF13. Pept_M24A_MAP1. 1 hit. PTHR10804. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
TIGRFAMs	TIGR00500. met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM1_BOVIN

Accession

Primary (citable) accession number: A6QLA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	August 21, 2007
Last modified:	September 1, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)