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Protein

L-lactate dehydrogenase 2

Gene

ldh2

Organism
Staphylococcus aureus (strain Newman)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to S.aureus growth during nitrosative stress in both aerobically and anaerobically cultured cells, despite playing a secondary role in this resistance mechanism.1 Publication

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase 2 (ldh2), L-lactate dehydrogenase 1 (ldh1)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei91SubstrateBy similarity1
Binding sitei123NAD or substrateBy similarity1
Binding sitei154SubstrateBy similarity1
Active sitei178Proton acceptorBy similarity1
Binding sitei231SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 42NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processStress response
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase 2 (EC:1.1.1.27)
Short name:
L-LDH 2
Gene namesi
Name:ldh2
Ordered Locus Names:NWMN_2499
OrganismiStaphylococcus aureus (strain Newman)
Taxonomic identifieri426430 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000006386 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003438431 – 319L-lactate dehydrogenase 2Add BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei222PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA6QK89.
SMRiA6QK89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

HOGENOMiHOG000213793.
KOiK00016.
OMAiDQWKNIH.

Family and domain databases

Gene3Di3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiView protein in InterPro
IPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR036291. NAD(P)-bd_dom_sf.
PfamiView protein in Pfam
PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiView protein in PROSITE
PS00064. L_LDH. 1 hit.

Sequencei

Sequence statusi: Complete.

A6QK89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTFGKKVVL IGDGSVGSSY AFAMVTQGVA DEFVIIDIAK DKVKADVQDL
60 70 80 90 100
NHGTVHSPSP VDVKAGEYED CKDADLVVIT AGAPQKPGET RLQLVEKNTK
110 120 130 140 150
IMKSIVKSVM DSGFDGYFLI AANPVDILTR FVKEYTGLPA ERVIGSGTVL
160 170 180 190 200
DSARLQYLIS QELGVAPSSV DASIIGEHGD TELAVWSQAN VAGISVYDTL
210 220 230 240 250
KEQTGSEAKA EEIYVNTRDA AYEIIQAKGS TYYGIALALM RISKAILNNE
260 270 280 290 300
NNVLNVSIQL DGQYGGHKGV YLGVPTLVNQ HGAVKIYEMP LSAEEQALFD
310
KSVKTLEDTF DSIKYLLED
Length:319
Mass (Da):34,420
Last modified:August 21, 2007 - v1
Checksum:i7779343BCA8006C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009351 Genomic DNA. Translation: BAF68771.1.
RefSeqiWP_000846637.1. NC_009641.1.

Genome annotation databases

EnsemblBacteriaiBAF68771; BAF68771; NWMN_2499.
GeneIDi31215364.
KEGGisae:NWMN_2499.

Similar proteinsi

Entry informationi

Entry nameiLDH2_STAAE
AccessioniPrimary (citable) accession number: A6QK89
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: August 21, 2007
Last modified: October 25, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families