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Protein

Thiamine-phosphate synthase

Gene

thiE

Organism
Staphylococcus aureus (strain Newman)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).UniRule annotation

Catalytic activityi

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751HMP-PPUniRule annotation
Metal bindingi76 – 761MagnesiumUniRule annotation
Metal bindingi95 – 951MagnesiumUniRule annotation
Binding sitei113 – 1131HMP-PPUniRule annotation
Binding sitei142 – 1421HMP-PPUniRule annotation
Binding sitei171 – 1711THZ-P; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. thiamine-phosphate diphosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-HAMAP
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSAUR426430:GIXC-2059-MONOMER.
UniPathwayiUPA00060; UER00141.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine-phosphate synthaseUniRule annotation (EC:2.5.1.3UniRule annotation)
Short name:
TP synthaseUniRule annotation
Short name:
TPSUniRule annotation
Alternative name(s):
Thiamine-phosphate pyrophosphorylaseUniRule annotation
Short name:
TMP pyrophosphorylaseUniRule annotation
Short name:
TMP-PPaseUniRule annotation
Gene namesi
Name:thiEUniRule annotation
Ordered Locus Names:NWMN_1995
OrganismiStaphylococcus aureus (strain Newman)
Taxonomic identifieri426430 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000006386: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213Thiamine-phosphate synthasePRO_1000071284Add
BLAST

Proteomic databases

PRIDEiA6QIT5.

Interactioni

Protein-protein interaction databases

STRINGi426430.NWMN_1995.

Structurei

3D structure databases

ProteinModelPortaliA6QIT5.
SMRiA6QIT5. Positions 4-209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni40 – 445HMP-PP bindingUniRule annotation
Regioni139 – 1413THZ-P bindingUniRule annotation
Regioni191 – 1922THZ-P bindingUniRule annotation

Sequence similaritiesi

Belongs to the thiamine-phosphate synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0352.
HOGENOMiHOG000155781.
KOiK00788.
OMAiVSAICHA.
OrthoDBiEOG6W19NW.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00097. TMP_synthase.
InterProiIPR013785. Aldolase_TIM.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF02581. TMP-TENI. 1 hit.
[Graphical view]
SUPFAMiSSF51391. SSF51391. 1 hit.
TIGRFAMsiTIGR00693. thiE. 1 hit.

Sequencei

Sequence statusi: Complete.

A6QIT5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFNQSYLNVY FICGTSDVPS HRTIHEVLEA ALKAGITLFQ FREKGESALK
60 70 80 90 100
GNDKLVLAKE LQHLCHQYDV PFIVNDDVSL AKEINADGIH VGQDDAKVKE
110 120 130 140 150
IAQYFTDKII GLSISDLDEY AKSDLTHVDY IGVGPIYPTP SKHDAHIPVG
160 170 180 190 200
PEMIATFKEM NPQLPIVAIG GINTNNVAPI VEAGANGISV ISAISKSENI
210
EKTVNRFKDF FNN
Length:213
Mass (Da):23,399
Last modified:August 21, 2007 - v1
Checksum:i8FEFB39D6EF82F94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009351 Genomic DNA. Translation: BAF68267.1.
RefSeqiYP_001333029.1. NC_009641.1.

Genome annotation databases

EnsemblBacteriaiBAF68267; BAF68267; NWMN_1995.
GeneIDi5332232.
KEGGisae:NWMN_1995.
PATRICi19587794. VBIStaAur133992_2182.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009351 Genomic DNA. Translation: BAF68267.1.
RefSeqiYP_001333029.1. NC_009641.1.

3D structure databases

ProteinModelPortaliA6QIT5.
SMRiA6QIT5. Positions 4-209.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi426430.NWMN_1995.

Proteomic databases

PRIDEiA6QIT5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF68267; BAF68267; NWMN_1995.
GeneIDi5332232.
KEGGisae:NWMN_1995.
PATRICi19587794. VBIStaAur133992_2182.

Phylogenomic databases

eggNOGiCOG0352.
HOGENOMiHOG000155781.
KOiK00788.
OMAiVSAICHA.
OrthoDBiEOG6W19NW.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00141.
BioCyciSAUR426430:GIXC-2059-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00097. TMP_synthase.
InterProiIPR013785. Aldolase_TIM.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF02581. TMP-TENI. 1 hit.
[Graphical view]
SUPFAMiSSF51391. SSF51391. 1 hit.
TIGRFAMsiTIGR00693. thiE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands."
    Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K.
    J. Bacteriol. 190:300-310(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Newman.

Entry informationi

Entry nameiTHIE_STAAE
AccessioniPrimary (citable) accession number: A6QIT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 21, 2007
Last modified: January 7, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.