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Protein

Thiamine-phosphate synthase

Gene

thiE

Organism
Staphylococcus aureus (strain Newman)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).UniRule annotation

Catalytic activityi

4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO2.UniRule annotation
4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2.UniRule annotation
4-amino-2-methyl-5-diphosphomethylpyrimidine + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamine-phosphate synthase (thiE)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75HMP-PPUniRule annotation1
Metal bindingi76MagnesiumUniRule annotation1
Metal bindingi95MagnesiumUniRule annotation1
Binding sitei113HMP-PPUniRule annotation1
Binding sitei142HMP-PPUniRule annotation1
Binding sitei171THZ-P; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00141

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine-phosphate synthaseUniRule annotation (EC:2.5.1.3UniRule annotation)
Short name:
TP synthaseUniRule annotation
Short name:
TPSUniRule annotation
Alternative name(s):
Thiamine-phosphate pyrophosphorylaseUniRule annotation
Short name:
TMP pyrophosphorylaseUniRule annotation
Short name:
TMP-PPaseUniRule annotation
Gene namesi
Name:thiEUniRule annotation
Ordered Locus Names:NWMN_1995
OrganismiStaphylococcus aureus (strain Newman)
Taxonomic identifieri426430 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000006386 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000712841 – 213Thiamine-phosphate synthaseAdd BLAST213

Structurei

3D structure databases

ProteinModelPortaliA6QIT5
SMRiA6QIT5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 44HMP-PP bindingUniRule annotation5
Regioni139 – 141THZ-P bindingUniRule annotation3
Regioni191 – 192THZ-P bindingUniRule annotation2

Sequence similaritiesi

Belongs to the thiamine-phosphate synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000155781
KOiK00788
OMAiITAFQFR

Family and domain databases

CDDicd00564 TMP_TenI, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00097 TMP_synthase, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR036206 ThiamineP_synth_sf
IPR022998 ThiamineP_synth_TenI
IPR034291 TMP_synthase
PfamiView protein in Pfam
PF02581 TMP-TENI, 1 hit
SUPFAMiSSF51391 SSF51391, 1 hit
TIGRFAMsiTIGR00693 thiE, 1 hit

Sequencei

Sequence statusi: Complete.

A6QIT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNQSYLNVY FICGTSDVPS HRTIHEVLEA ALKAGITLFQ FREKGESALK
60 70 80 90 100
GNDKLVLAKE LQHLCHQYDV PFIVNDDVSL AKEINADGIH VGQDDAKVKE
110 120 130 140 150
IAQYFTDKII GLSISDLDEY AKSDLTHVDY IGVGPIYPTP SKHDAHIPVG
160 170 180 190 200
PEMIATFKEM NPQLPIVAIG GINTNNVAPI VEAGANGISV ISAISKSENI
210
EKTVNRFKDF FNN
Length:213
Mass (Da):23,399
Last modified:August 21, 2007 - v1
Checksum:i8FEFB39D6EF82F94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009351 Genomic DNA Translation: BAF68267.1
RefSeqiWP_000483153.1, NZ_CP023390.1

Genome annotation databases

EnsemblBacteriaiBAF68267; BAF68267; NWMN_1995
KEGGisae:NWMN_1995

Similar proteinsi

Entry informationi

Entry nameiTHIE_STAAE
AccessioniPrimary (citable) accession number: A6QIT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 21, 2007
Last modified: March 28, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health