A6QIE0 (NADE_STAAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	NWMN_1850

Organism

Staphylococcus aureus (strain Newman) [Complete proteome] [HAMAP]

Taxonomic identifier

426430 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077618
Regions
	Nucleotide binding	47 – 54	8	ATP By similarity
Sites
	Active site	49	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6QIE0 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: BA4A032FFEFFD4A2	FASTA	273	30,697
	10 20 30 40 50 60 MSKLQDVIVQ EMKVKKRIDS AEEIMELKQF IKNYVQSHSF IKSLVLGISG GQDSTLVGKL 70 80 90 100 110 120 VQMSVNELRE EGIDCTFIAV KLPYGVQKDA DEVEQALRFI EPDEIVTVNI KPAVDQSVQS 130 140 150 160 170 180 LKEAGIVLTD FQKGNEKARE RMKVQFSIAS NRQGIVVGTD HSAENITGFY TKYGDGAADI 190 200 210 220 230 240 APIFGLNKRQ GRQLLAYLGA PKELYEKTPT ADLEDDKPQL PDEDALGVTY EAIDNYLEGK 250 260 270 PVTPEEQKVI ENHYIRNAHK RELAYTRYTW PKS

References

[1]

"Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands." Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K. J. Bacteriol. 190:300-310(2008) [PubMed: 17951380] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Newman.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009351 Genomic DNA. Translation: BAF68122.1.
RefSeq	YP_001332884.1. NC_009641.1.
3D structure databases
ProteinModelPortal	A6QIE0.
SMR	A6QIE0. Positions 4-268.
ModBase	Search...
Protein-protein interaction databases
STRING	A6QIE0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000031221; EBSTAP00000030091; EBSTAG00000031219.
GeneID	5331121.
GenomeReviews	Gene locus NWMN_1850 in contig AP009351_GR.
KEGG	sae:NWMN_1850.
PATRIC	19587446. VBIStaAur133992_2013.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
GeneTree	EBGT00050000025036.
HOGENOM	HBG351567.
OMA	IAQYEIA.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	SAUR426430:NWMN_1850-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_STAAE

Accession

Primary (citable) accession number: A6QIE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program