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Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Staphylococcus aureus (strain Newman)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36TyrosineUniRule annotation1
Binding sitei170TyrosineUniRule annotation1
Binding sitei174TyrosineUniRule annotation1
Binding sitei234ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.1 3352

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligaseUniRule annotation (EC:6.1.1.1UniRule annotation)
Alternative name(s):
Tyrosyl-tRNA synthetaseUniRule annotation
Short name:
TyrRSUniRule annotation
Gene namesi
Name:tyrSUniRule annotation
Ordered Locus Names:NWMN_1622
OrganismiStaphylococcus aureus (strain Newman)
Taxonomic identifieri426430 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000006386 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000886301 – 420Tyrosine--tRNA ligaseAdd BLAST420

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

Secondary structure

1420
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 11Combined sources8
Beta strandi16 – 20Combined sources5
Helixi21 – 30Combined sources10
Beta strandi34 – 39Combined sources6
Beta strandi42 – 45Combined sources4
Helixi48 – 62Combined sources15
Beta strandi66 – 71Combined sources6
Helixi75 – 77Combined sources3
Helixi93 – 110Combined sources18
Beta strandi114 – 118Combined sources5
Beta strandi120 – 123Combined sources4
Helixi125 – 128Combined sources4
Helixi133 – 139Combined sources7
Helixi141 – 143Combined sources3
Helixi146 – 150Combined sources5
Helixi153 – 155Combined sources3
Helixi156 – 159Combined sources4
Turni160 – 162Combined sources3
Helixi165 – 185Combined sources21
Beta strandi187 – 193Combined sources7
Helixi194 – 196Combined sources3
Helixi197 – 211Combined sources15
Beta strandi217 – 221Combined sources5
Beta strandi227 – 229Combined sources3
Beta strandi236 – 239Combined sources4
Beta strandi241 – 243Combined sources3
Turni244 – 246Combined sources3
Helixi249 – 257Combined sources9
Helixi261 – 271Combined sources11
Helixi276 – 288Combined sources13
Helixi290 – 292Combined sources3
Helixi294 – 308Combined sources15
Helixi310 – 323Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JIIX-ray3.20A1-420[»]
1JIJX-ray3.20A1-420[»]
1JIKX-ray2.80A1-420[»]
1JILX-ray2.20A1-420[»]
ProteinModelPortaliA6QHR2
SMRiA6QHR2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA6QHR2

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini353 – 420S4 RNA-bindingUniRule annotationAdd BLAST68

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 50"HIGH" region10
Motifi231 – 235"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000242790
KOiK01866
OMAiVNYMMAK

Family and domain databases

CDDicd00165 S4, 1 hit
Gene3Di3.10.290.10, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_02006 Tyr_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002942 S4_RNA-bd
IPR036986 S4_RNA-bd_sf
IPR002307 Tyr-tRNA-ligase
IPR024088 Tyr-tRNA-ligase_bac-type
IPR024107 Tyr-tRNA-ligase_bac_1
PANTHERiPTHR11766 PTHR11766, 1 hit
PfamiView protein in Pfam
PF01479 S4, 1 hit
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01040 TRNASYNTHTYR
SMARTiView protein in SMART
SM00363 S4, 1 hit
TIGRFAMsiTIGR00234 tyrS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
PS50889 S4, 1 hit

Sequencei

Sequence statusi: Complete.

A6QHR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNVLIEDLK WRGLIYQQTD EQGIEDLLNK EQVTLYCGAD PTADSLHIGH
60 70 80 90 100
LLPFLTLRRF QEHGHRPIVL IGGGTGMIGD PSGKSEERVL QTEEQVDKNI
110 120 130 140 150
EGISKQMHNI FEFGTDHGAV LVNNRDWLGQ ISLISFLRDY GKHVGVNYML
160 170 180 190 200
GKDSIQSRLE HGISYTEFTY TILQAIDFGH LNRELNCKIQ VGGSDQWGNI
210 220 230 240 250
TSGIELMRRM YGQTDAYGLT IPLVTKSDGK KFGKSESGAV WLDAEKTSPY
260 270 280 290 300
EFYQFWINQS DEDVIKFLKY FTFLGKEEID RLEQSKNEAP HLREAQKTLA
310 320 330 340 350
EEVTKFIHGE DALNDAIRIS QALFSGDLKS LSAKELKDGF KDVPQVTLSN
360 370 380 390 400
DTTNIVEVLI ETGISPSKRQ AREDVNNGAI YINGERQQDV NYALAPEDKI
410 420
DGEFTIIRRG KKKYFMVNYQ
Length:420
Mass (Da):47,598
Last modified:August 21, 2007 - v1
Checksum:iB6916EA4BE4B21B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009351 Genomic DNA Translation: BAF67894.1
RefSeqiWP_000186029.1, NZ_CP023390.1

Genome annotation databases

EnsemblBacteriaiBAF67894; BAF67894; NWMN_1622
KEGGisae:NWMN_1622

Similar proteinsi

Entry informationi

Entry nameiSYY_STAAE
AccessioniPrimary (citable) accession number: A6QHR2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 21, 2007
Last modified: March 28, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

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