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Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Staphylococcus aureus (strain Newman)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361TyrosineUniRule annotation
Binding sitei170 – 1701TyrosineUniRule annotation
Binding sitei174 – 1741TyrosineUniRule annotation
Binding sitei234 – 2341ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciSAUR426430:GIXC-1645-MONOMER.
BRENDAi6.1.1.1. 3352.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligaseUniRule annotation (EC:6.1.1.1UniRule annotation)
Alternative name(s):
Tyrosyl-tRNA synthetaseUniRule annotation
Short name:
TyrRSUniRule annotation
Gene namesi
Name:tyrSUniRule annotation
Ordered Locus Names:NWMN_1622
OrganismiStaphylococcus aureus (strain Newman)
Taxonomic identifieri426430 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000006386 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 420420Tyrosine--tRNA ligasePRO_1000088630Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Chemistry

BindingDBiA6QHR2.

Structurei

Secondary structure

1
420
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 118Combined sources
Beta strandi16 – 205Combined sources
Helixi21 – 3010Combined sources
Beta strandi34 – 396Combined sources
Beta strandi42 – 454Combined sources
Helixi48 – 6215Combined sources
Beta strandi66 – 716Combined sources
Helixi75 – 773Combined sources
Helixi93 – 11018Combined sources
Beta strandi114 – 1185Combined sources
Beta strandi120 – 1234Combined sources
Helixi125 – 1284Combined sources
Helixi133 – 1397Combined sources
Helixi141 – 1433Combined sources
Helixi146 – 1505Combined sources
Helixi153 – 1553Combined sources
Helixi156 – 1594Combined sources
Turni160 – 1623Combined sources
Helixi165 – 18521Combined sources
Beta strandi187 – 1937Combined sources
Helixi194 – 1963Combined sources
Helixi197 – 21115Combined sources
Beta strandi217 – 2215Combined sources
Beta strandi227 – 2293Combined sources
Beta strandi236 – 2394Combined sources
Beta strandi241 – 2433Combined sources
Turni244 – 2463Combined sources
Helixi249 – 2579Combined sources
Helixi261 – 27111Combined sources
Helixi276 – 28813Combined sources
Helixi290 – 2923Combined sources
Helixi294 – 30815Combined sources
Helixi310 – 32314Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JIIX-ray3.20A1-420[»]
1JIJX-ray3.20A1-420[»]
1JIKX-ray2.80A1-420[»]
1JILX-ray2.20A1-420[»]
ProteinModelPortaliA6QHR2.
SMRiA6QHR2. Positions 2-420.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA6QHR2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini353 – 42068S4 RNA-bindingUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5010"HIGH" region
Motifi231 – 2355"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily.UniRule annotation
Contains 1 S4 RNA-binding domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000242790.
KOiK01866.
OMAiGKHFPVN.
OrthoDBiEOG6B09VR.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02006. Tyr_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QHR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNVLIEDLK WRGLIYQQTD EQGIEDLLNK EQVTLYCGAD PTADSLHIGH
60 70 80 90 100
LLPFLTLRRF QEHGHRPIVL IGGGTGMIGD PSGKSEERVL QTEEQVDKNI
110 120 130 140 150
EGISKQMHNI FEFGTDHGAV LVNNRDWLGQ ISLISFLRDY GKHVGVNYML
160 170 180 190 200
GKDSIQSRLE HGISYTEFTY TILQAIDFGH LNRELNCKIQ VGGSDQWGNI
210 220 230 240 250
TSGIELMRRM YGQTDAYGLT IPLVTKSDGK KFGKSESGAV WLDAEKTSPY
260 270 280 290 300
EFYQFWINQS DEDVIKFLKY FTFLGKEEID RLEQSKNEAP HLREAQKTLA
310 320 330 340 350
EEVTKFIHGE DALNDAIRIS QALFSGDLKS LSAKELKDGF KDVPQVTLSN
360 370 380 390 400
DTTNIVEVLI ETGISPSKRQ AREDVNNGAI YINGERQQDV NYALAPEDKI
410 420
DGEFTIIRRG KKKYFMVNYQ
Length:420
Mass (Da):47,598
Last modified:August 21, 2007 - v1
Checksum:iB6916EA4BE4B21B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009351 Genomic DNA. Translation: BAF67894.1.
RefSeqiWP_000186029.1. NC_009641.1.

Genome annotation databases

EnsemblBacteriaiBAF67894; BAF67894; NWMN_1622.
KEGGisae:NWMN_1622.
PATRICi19586853. VBIStaAur133992_1755.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009351 Genomic DNA. Translation: BAF67894.1.
RefSeqiWP_000186029.1. NC_009641.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JIIX-ray3.20A1-420[»]
1JIJX-ray3.20A1-420[»]
1JIKX-ray2.80A1-420[»]
1JILX-ray2.20A1-420[»]
ProteinModelPortaliA6QHR2.
SMRiA6QHR2. Positions 2-420.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiA6QHR2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF67894; BAF67894; NWMN_1622.
KEGGisae:NWMN_1622.
PATRICi19586853. VBIStaAur133992_1755.

Phylogenomic databases

HOGENOMiHOG000242790.
KOiK01866.
OMAiGKHFPVN.
OrthoDBiEOG6B09VR.

Enzyme and pathway databases

BioCyciSAUR426430:GIXC-1645-MONOMER.
BRENDAi6.1.1.1. 3352.

Miscellaneous databases

EvolutionaryTraceiA6QHR2.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02006. Tyr_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands."
    Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K.
    J. Bacteriol. 190:300-310(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Newman.

Entry informationi

Entry nameiSYY_STAAE
AccessioniPrimary (citable) accession number: A6QHR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 21, 2007
Last modified: March 16, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.