A6QHR2 (SYY_STAAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	NWMN_1622

Organism

Staphylococcus aureus (strain Newman) [Complete proteome] [HAMAP]

Taxonomic identifier

426430 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP-Rule MF_02006
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP-Rule MF_02006
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 420

420

Tyrosine--tRNA ligase HAMAP-Rule MF_02006

PRO_1000088630

Regions

Domain

353 – 420

68

S4 RNA-binding

Motif

41 – 50

10

"HIGH" region HAMAP-Rule MF_02006

Motif

231 – 235

5

"KMSKS" region HAMAP-Rule MF_02006

Sites

Binding site

36

1

Tyrosine By similarity

Binding site

170

1

Tyrosine By similarity

Binding site

174

1

Tyrosine By similarity

Binding site

234

1

ATP By similarity

Secondary structure

1

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420

Helix Strand Turn

Details...

Helix

4 – 11

8

Beta strand

16 – 20

5

Helix

21 – 30

10

Beta strand

34 – 39

6

Beta strand

42 – 45

4

Helix

48 – 62

15

Beta strand

66 – 71

6

Helix

75 – 77

3

Helix

93 – 110

18

Beta strand

114 – 118

5

Beta strand

120 – 123

4

Helix

125 – 128

4

Helix

133 – 139

7

Helix

141 – 143

3

Helix

146 – 150

5

Helix

153 – 155

3

Helix

156 – 159

4

Turn

160 – 162

3

Helix

165 – 185

21

Beta strand

187 – 193

7

Helix

194 – 196

3

Helix

197 – 211

15

Beta strand

217 – 221

5

Beta strand

227 – 229

3

Beta strand

236 – 239

4

Beta strand

241 – 243

3

Turn

244 – 246

3

Helix

249 – 257

9

Helix

261 – 271

11

Helix

276 – 288

13

Helix

290 – 292

3

Helix

294 – 308

15

Helix

310 – 323

14

Sequences

	Sequence		Length	Mass (Da)
	A6QHR2 [UniParc]. Last modified August 21, 2007. Version 1. Checksum: B6916EA4BE4B21B2	FASTA	420	47,598
	10 20 30 40 50 60 MTNVLIEDLK WRGLIYQQTD EQGIEDLLNK EQVTLYCGAD PTADSLHIGH LLPFLTLRRF 70 80 90 100 110 120 QEHGHRPIVL IGGGTGMIGD PSGKSEERVL QTEEQVDKNI EGISKQMHNI FEFGTDHGAV 130 140 150 160 170 180 LVNNRDWLGQ ISLISFLRDY GKHVGVNYML GKDSIQSRLE HGISYTEFTY TILQAIDFGH 190 200 210 220 230 240 LNRELNCKIQ VGGSDQWGNI TSGIELMRRM YGQTDAYGLT IPLVTKSDGK KFGKSESGAV 250 260 270 280 290 300 WLDAEKTSPY EFYQFWINQS DEDVIKFLKY FTFLGKEEID RLEQSKNEAP HLREAQKTLA 310 320 330 340 350 360 EEVTKFIHGE DALNDAIRIS QALFSGDLKS LSAKELKDGF KDVPQVTLSN DTTNIVEVLI 370 380 390 400 410 420 ETGISPSKRQ AREDVNNGAI YINGERQQDV NYALAPEDKI DGEFTIIRRG KKKYFMVNYQ

References

[1]	"Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands." Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K. J. Bacteriol. 190:300-310(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Newman.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AP009351 Genomic DNA. Translation: BAF67894.1.

RefSeq

YP_001332656.1. NC_009641.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JII	X-ray	3.20	A	1-420	[»]
1JIJ	X-ray	3.20	A	1-420	[»]
1JIK	X-ray	2.80	A	1-420	[»]
1JIL	X-ray	2.20	A	1-420	[»]

ProteinModelPortal

A6QHR2.

SMR

A6QHR2. Positions 2-419.

ModBase

Search...

Protein-protein interaction databases

STRING

426430.NWMN_1622.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAF67894; BAF67894; NWMN_1622.

GeneID

5330953.

KEGG

sae:NWMN_1622.

PATRIC

19586853. VBIStaAur133992_1755.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0162.

HOGENOM

HOG000242790.

KO

K01866.

OMA

GKKKHVL.

ProtClustDB

PRK05912.

Enzyme and pathway databases

BioCyc

SAUR426430:GIXC-1623-MONOMER.

Family and domain databases

Gene3D

3.10.290.10. 1 hit.
3.40.50.620. 1 hit.

HAMAP

MF_02006. Tyr_tRNA_synth_type1.

InterPro

IPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]

PANTHER

PTHR11766. PTHR11766. 1 hit.

Pfam

PF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]

PRINTS

PR01040. TRNASYNTHTYR.

SMART

SM00363. S4. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00234. tyrS. 1 hit.

PROSITE

PS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

A6QHR2.

EvolutionaryTrace

A6QHR2.

Entry information

Entry name

SYY_STAAE

Accession

Primary (citable) accession number: A6QHR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 21, 2007
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program