Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Staphylococcus aureus (strain Newman)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36TyrosineUniRule annotation1
Binding sitei170TyrosineUniRule annotation1
Binding sitei174TyrosineUniRule annotation1
Binding sitei234ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BRENDAi6.1.1.1. 3352.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligaseUniRule annotation (EC:6.1.1.1UniRule annotation)
Alternative name(s):
Tyrosyl-tRNA synthetaseUniRule annotation
Short name:
TyrRSUniRule annotation
Gene namesi
Name:tyrSUniRule annotation
Ordered Locus Names:NWMN_1622
OrganismiStaphylococcus aureus (strain Newman)
Taxonomic identifieri426430 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000006386 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000886301 – 420Tyrosine--tRNA ligaseAdd BLAST420

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

Secondary structure

1420
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 11Combined sources8
Beta strandi16 – 20Combined sources5
Helixi21 – 30Combined sources10
Beta strandi34 – 39Combined sources6
Beta strandi42 – 45Combined sources4
Helixi48 – 62Combined sources15
Beta strandi66 – 71Combined sources6
Helixi75 – 77Combined sources3
Helixi93 – 110Combined sources18
Beta strandi114 – 118Combined sources5
Beta strandi120 – 123Combined sources4
Helixi125 – 128Combined sources4
Helixi133 – 139Combined sources7
Helixi141 – 143Combined sources3
Helixi146 – 150Combined sources5
Helixi153 – 155Combined sources3
Helixi156 – 159Combined sources4
Turni160 – 162Combined sources3
Helixi165 – 185Combined sources21
Beta strandi187 – 193Combined sources7
Helixi194 – 196Combined sources3
Helixi197 – 211Combined sources15
Beta strandi217 – 221Combined sources5
Beta strandi227 – 229Combined sources3
Beta strandi236 – 239Combined sources4
Beta strandi241 – 243Combined sources3
Turni244 – 246Combined sources3
Helixi249 – 257Combined sources9
Helixi261 – 271Combined sources11
Helixi276 – 288Combined sources13
Helixi290 – 292Combined sources3
Helixi294 – 308Combined sources15
Helixi310 – 323Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JIIX-ray3.20A1-420[»]
1JIJX-ray3.20A1-420[»]
1JIKX-ray2.80A1-420[»]
1JILX-ray2.20A1-420[»]
ProteinModelPortaliA6QHR2.
SMRiA6QHR2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA6QHR2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini353 – 420S4 RNA-bindingUniRule annotationAdd BLAST68

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 50"HIGH" region10
Motifi231 – 235"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily.UniRule annotation
Contains 1 S4 RNA-binding domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000242790.
KOiK01866.
OMAiGKHFPVN.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02006. Tyr_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6QHR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNVLIEDLK WRGLIYQQTD EQGIEDLLNK EQVTLYCGAD PTADSLHIGH
60 70 80 90 100
LLPFLTLRRF QEHGHRPIVL IGGGTGMIGD PSGKSEERVL QTEEQVDKNI
110 120 130 140 150
EGISKQMHNI FEFGTDHGAV LVNNRDWLGQ ISLISFLRDY GKHVGVNYML
160 170 180 190 200
GKDSIQSRLE HGISYTEFTY TILQAIDFGH LNRELNCKIQ VGGSDQWGNI
210 220 230 240 250
TSGIELMRRM YGQTDAYGLT IPLVTKSDGK KFGKSESGAV WLDAEKTSPY
260 270 280 290 300
EFYQFWINQS DEDVIKFLKY FTFLGKEEID RLEQSKNEAP HLREAQKTLA
310 320 330 340 350
EEVTKFIHGE DALNDAIRIS QALFSGDLKS LSAKELKDGF KDVPQVTLSN
360 370 380 390 400
DTTNIVEVLI ETGISPSKRQ AREDVNNGAI YINGERQQDV NYALAPEDKI
410 420
DGEFTIIRRG KKKYFMVNYQ
Length:420
Mass (Da):47,598
Last modified:August 21, 2007 - v1
Checksum:iB6916EA4BE4B21B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009351 Genomic DNA. Translation: BAF67894.1.
RefSeqiWP_000186029.1. NC_009641.1.

Genome annotation databases

EnsemblBacteriaiBAF67894; BAF67894; NWMN_1622.
GeneIDi28380681.
KEGGisae:NWMN_1622.
PATRICi19586853. VBIStaAur133992_1755.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009351 Genomic DNA. Translation: BAF67894.1.
RefSeqiWP_000186029.1. NC_009641.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JIIX-ray3.20A1-420[»]
1JIJX-ray3.20A1-420[»]
1JIKX-ray2.80A1-420[»]
1JILX-ray2.20A1-420[»]
ProteinModelPortaliA6QHR2.
SMRiA6QHR2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF67894; BAF67894; NWMN_1622.
GeneIDi28380681.
KEGGisae:NWMN_1622.
PATRICi19586853. VBIStaAur133992_1755.

Phylogenomic databases

HOGENOMiHOG000242790.
KOiK01866.
OMAiGKHFPVN.

Enzyme and pathway databases

BRENDAi6.1.1.1. 3352.

Miscellaneous databases

EvolutionaryTraceiA6QHR2.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02006. Tyr_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYY_STAAE
AccessioniPrimary (citable) accession number: A6QHR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 21, 2007
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.