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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Staphylococcus aureus (strain Newman)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotation
  • thiamine diphosphateUniRule annotationNote: Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi: 1,4-dihydroxy-2-naphthoate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD)
  3. Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH)
  4. o-succinylbenzoate synthase (menC)
  5. 2-succinylbenzoate--CoA ligase (menE)
  6. 1,4-dihydroxy-2-naphthoyl-CoA synthase (memB)
  7. no protein annotated in this organism
This subpathway is part of the pathway 1,4-dihydroxy-2-naphthoate biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate, the pathway 1,4-dihydroxy-2-naphthoate biosynthesis and in Quinol/quinone metabolism.

Pathwayi: menaquinone biosynthesis

This protein is involved in the pathway menaquinone biosynthesis, which is part of Quinol/quinone metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processMenaquinone biosynthesis
LigandMagnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

UniPathwayiUPA00079
UPA01057; UER00164

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenDUniRule annotation
Gene namesi
Name:menDUniRule annotation
Synonyms:memD
Ordered Locus Names:NWMN_0913
OrganismiStaphylococcus aureus (strain Newman)
Taxonomic identifieri426430 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000006386 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003418601 – 5572-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseAdd BLAST557

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA6QFQ3
SMRiA6QFQ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218359
KOiK02551
OMAiIFRILPG

Family and domain databases

HAMAPiMF_01659 MenD, 1 hit
InterProiView protein in InterPro
IPR004433 MenaQ_synth_MenD
IPR032264 MenD_middle
IPR029061 THDP-binding
IPR012001 Thiamin_PyroP_enz_TPP-bd_dom
IPR011766 TPP_enzyme-bd_C
PANTHERiPTHR42916 PTHR42916, 1 hit
PfamiView protein in Pfam
PF02775 TPP_enzyme_C, 1 hit
PF16582 TPP_enzyme_M_2, 1 hit
PF02776 TPP_enzyme_N, 1 hit
PIRSFiPIRSF004983 MenD, 1 hit
SUPFAMiSSF52518 SSF52518, 2 hits
TIGRFAMsiTIGR00173 menD, 1 hit

Sequencei

Sequence statusi: Complete.

A6QFQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNHKAALTK QVFTFASELY AYGVREVVIS PGSRSTPLAL AFEAHPNIKT
60 70 80 90 100
WIHPDERSAA FFAVGLIKGS ERPVAILCTS GTAAANYTPA IAESQISRIP
110 120 130 140 150
LIVLTSDRPH ELRSVGAPQA INQVNMFNNY VSYEFDMPIA DDSKETIDAI
160 170 180 190 200
YYQMQIASQY LYGPHKGPIH FNLPFRDPLT PDLNATELLT SEMKILPHYQ
210 220 230 240 250
KSIDASALRH ILNKKKGLII VGDMQHQEVD QILTYSTIYD LPILADPLSH
260 270 280 290 300
LRKFDHPNVI CTYDLLFRSG LDLNVDFVIR VGKPVISKKL NQWLKKTDAF
310 320 330 340 350
QILVQNNDKI DVFPIAPDIS YEISANDFFR SLMEDTTVNR VSWLEKWQCL
360 370 380 390 400
EKKGRKEIKC YLEQATDESA FVGELIKKTS EKDALFISNS MPIRDVDNLL
410 420 430 440 450
LNKNIDVYAN RGANGIDGIV STALGMAVHK RITLLIGDLS FYHDMNGLLM
460 470 480 490 500
SKLNNIQMNI VLLNNDGGGI FSYLPQKESA TDYFERLFGT PTGLDFEYTA
510 520 530 540 550
KLYQFDFKRF NSVSEFKNAT LLSETSTIYE LITNREDNFK QHQILYQKLS

EMIHDTL
Length:557
Mass (Da):63,078
Last modified:August 21, 2007 - v1
Checksum:iC99072666F0769EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009351 Genomic DNA Translation: BAF67185.1
RefSeqiWP_000526687.1, NZ_CP023390.1

Genome annotation databases

EnsemblBacteriaiBAF67185; BAF67185; NWMN_0913
KEGGisae:NWMN_0913

Similar proteinsi

Entry informationi

Entry nameiMEND_STAAE
AccessioniPrimary (citable) accession number: A6QFQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: August 21, 2007
Last modified: March 28, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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