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A6QFD6

- LIPA_STAAE

UniProt

A6QFD6 - LIPA_STAAE

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Protein

Lipoyl synthase

Gene
lipA, NWMN_0796
Organism
Staphylococcus aureus (strain Newman)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi41 – 411Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi46 – 461Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi52 – 521Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi68 – 681Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi72 – 721Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi75 – 751Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSAUR426430:GIXC-816-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:NWMN_0796
OrganismiStaphylococcus aureus (strain Newman)
Taxonomic identifieri426430 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000006386: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Lipoyl synthaseUniRule annotationPRO_1000071730Add
BLAST

Proteomic databases

PRIDEiA6QFD6.

Interactioni

Protein-protein interaction databases

STRINGi426430.NWMN_0796.

Structurei

3D structure databases

ProteinModelPortaliA6QFD6.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiRKVDCDI.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A6QFD6-1 [UniParc]FASTAAdd to Basket

« Hide

MATKNEEILR KPDWLKIKLN TNENYTGLKK MMREKNLNTV CEEAKCPNIH    50
ECWGARRTAT FMILGAVCTR ACRFCAVKTG LPNELDLNEP ERVAESVELM 100
NLKHVVITAV ARDDLRDAGS NVYAETVRKV RERNPFTTIE ILPSDMGGDY 150
DALETLMASR PDILNHNIET VRRLTPRVRA RATYDRTLEF LRRSKELQPD 200
IPTKSSIMVG LGETIEEIYE TMDDLRANDV DILTIGQYLQ PSRKHLKVQK 250
YYTPLEFGKL RKVAMDKGFK HCQAGPLVRS SYHADEQVNE AAKEKQRQGE 300
AQLNS 305
Length:305
Mass (Da):34,885
Last modified:August 21, 2007 - v1
Checksum:i0911CD221177702B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009351 Genomic DNA. Translation: BAF67068.1.
RefSeqiYP_001331830.1. NC_009641.1.

Genome annotation databases

EnsemblBacteriaiBAF67068; BAF67068; NWMN_0796.
GeneIDi5330470.
KEGGisae:NWMN_0796.
PATRICi19585073. VBIStaAur133992_0865.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009351 Genomic DNA. Translation: BAF67068.1 .
RefSeqi YP_001331830.1. NC_009641.1.

3D structure databases

ProteinModelPortali A6QFD6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 426430.NWMN_0796.

Proteomic databases

PRIDEi A6QFD6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAF67068 ; BAF67068 ; NWMN_0796 .
GeneIDi 5330470.
KEGGi sae:NWMN_0796.
PATRICi 19585073. VBIStaAur133992_0865.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi RKVDCDI.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

BioCyci SAUR426430:GIXC-816-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands."
    Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K.
    J. Bacteriol. 190:300-310(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Newman.

Entry informationi

Entry nameiLIPA_STAAE
AccessioniPrimary (citable) accession number: A6QFD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 21, 2007
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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